Enzyme Inhibitory and Antioxidant Activity of Combination of Two Edible Mushrooms of Ganoderma lucidum and Pleurotus ostreatus doi.org/10.26538/tjnpr/v2i7.3
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Abstract
Edible mushrooms such as Ganoderma lucidum and Pleurotus ostreatus are of great significance in traditional medicine. This study evaluated the in vitro antioxidant and α-amylase inhibition potentials of the hydro-methanol extracts of the fruiting bodies of Ganoderma lucidum (GL), Pleurotus ostreatus (PO) and their combination (GL+PO). The extracts were obtained by hydromethanol (1:40) extraction. The extracts showed a dose-dependent antioxidant and α-amylase inhibitory activities. The combined extracts (GL+PO) exhibited higher antioxidant and α-amylase inhibitory activities than that shown by the individual extract. The combination of GL and PO exhibited maximum (98.65%) antioxidant activity at the highest concentration. GL extract showed the highest amount of total flavonoid (6.58 mg quercetin Eq/g) and total phenolic content (13.67mg catechol Eq/g) and strong DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging (24.58µg/mL), superoxide scavenging (652.39 µg/mL), and hydroxyl radical scavenging (555.43
µg/mL) activities while PO exhibited 26.36 µg/mL, 775.65 µg/mL, and 769.23 µg/mL, correspondingly. The combination of GL and PO showed the highest free radical scavenging capabilities (15.01 µg/mL, 495.59 µg/mL, and 312.85 µg/mL for DPPH radical scavenging,
superoxide scavenging and hydroxyl radical scavenging activities, respectively). Methanol extracts of mushroom, GL, PO, and GL+PO showed a dose-dependent enzyme inhibitory potential against α-amylase activity with IC50 of 386.04 µg/mL for GL, 391.74 µg/mL for PO, and 363.07 µg/mL for GL+PO, while that of acarbose was 269.15 µg/mL. The results indicate that the combination of Ganoderma lucidum and Pleurotus ostreatus extracts has significantly higher antioxidant and alfa-amylase inhibitory activities compared to the single individual extract.
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