Effect of pH Variation of Ethanol Solvent in Purple Sweet Potato (Ipomoea batatas L.) Extract on Hepatoprotective Activity of White Rats (Rattus novergicus)
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Abstract
In particular, PM 2.5 (air pollution) passes through the alveoli into the blood circulation and can reach the liver for catabolism, leading to liver damage. The hepatoprotective agents may be purple sweet potato tubers (Ipomoea batatas L.) with bioactive anthocyanin compounds. Anthocyanins are unstable at pH values ≥7. This study aimed to determine the effect of pH variations of the ethanol solvent (without acidification, pH 2.5 and pH 1.5) in the extraction of purple sweet potato tubers on hepatoprotective activity. For the study, the experimental animals were divided into six different groups, including a standard control group, a negative control group with administered carboxy methyl cellulose (CMC) 1% /kg body weight, a positive control group with 100 mg/kg body weight of Silymarin (SMR), purple sweet potato extract (PSPE) 400 mg/kg body weight group without acidification and two groups with pH 1.5 and pH 2.5 with 400 mg/kg bw of PSPE. Paracetamol (PCM) was administered on the seventh day (except for the standard group) with 3000 mg/kg body weight one hour after the last oral treatment. PSPE has hepatoprotective activity and affects acidic pH in biochemical tests, total protein, albumin, liver index and histology. Results revealed that treatment with pH 1.5 and 400 mg/kg bw of PSPE significantly restored the liver function markers to their average values, including serum enzyme levels, and increased total protein and albumin levels is an indication of the ability to restore synthesis function in paracetamol-induced liver damage slowly.
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