Counteracting Paracetamol-Induced Hepatotoxicity with Black Shallot Extract: An Animal Model Investigation


  • Tran T.P. Nhung Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam
  • Le P.T. Quoc Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam


Hepatoprotective effects, Hepatotoxicity, Paracetamol, Liver injury, Allium ascalonicum L.


Black shallot is a novel product of shallots (Allium ascalonicum L.), a traditional plant used worldwide for both culinary and folk medicinal purposes through controlled temperature and humidity fermentation. Paracetamol, a commonly used pain reliever and fever reducer, is a leading cause of poisoning in children and can result in liver failure. This study investigates the hepatoprotective effects of black shallot against acute paracetamol-related toxicity. Swiss albino mice were divided into six treatments and administered normal saline (10 mL/kg), N-acetylcysteine (50 mg/kg), and black shallot extract (EAAS) at doses of 200, 300, and 400 mg/kg for seven days, followed by paracetamol (PCM, 3000 mg/kg). Liver toxicity assessments were conducted 48 hours after PCM administration. The total phenolic and flavonoid contents of EAAS were found to be 22.74 mg/100g and 38.3 mg/100g, respectively. EAAS significantly reduced (p < 0.05) WBC counts, serum bilirubin levels (0.44 ± 0.02 µmol/L), and liver enzyme activities (AST, ALP, and ALT), as well as MDA levels (1.87 ± 0.07 nmol/mg protein) mediated by PCM. Similarly, EAAS significantly increased (p < 0.05) the activities of CAT (13.73 ± 1.07 mM/min/g tissue), GSH (4.01 ± 0.04 nM/mg tissue), and SOD (22.39 ± 4.27 mM/min/mg tissue) in PCM-intoxicated mice. Morphological and histological changes related to PCM-induced liver toxicity were also improved by EAAS. Overall, the oxidative and histological evaluations suggest that black shallot extract may have a preventive effect against acute paracetamol-induced liver damage.


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How to Cite

Nhung, T. T., & Quoc, L. P. (2024). Counteracting Paracetamol-Induced Hepatotoxicity with Black Shallot Extract: An Animal Model Investigation: Tropical Journal of Natural Product Research (TJNPR), 8(1), 5875–5880. Retrieved from