Potential Inhibitory Effects on Human Platelet Aggregation and blood Coagulation of the Aerial Part of Portulaca oleracea L http://www.doi.org/10.26538/tjnpr/v8i1.42
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Abstract
Portulaca oleracea L. is a vegetable plant traditionally used to treat several conditions. A wide range of bioactivities of P. oleracea were reported. However, its potential in the treatment and prevention of heart diseases has rarely been documented. This study reports, for the first time, the inhibitory effect on human platelet aggregation and blood coagulation of the aerial part of P. oleracea L. The dried aerial part of P. oleracea was macerated with 80% ethanol, suspended in the water and then successively fractionated with n-hexane, ethyl acetate. Blood samples were collected from healthy volunteers. The antiplatelet effects of extracts were evaluated using the turbidimetric method. The anticoagulant activity was assessed by measuring prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time. The results showed that the aerial part of P. oleracea had potent inhibitory effects on human platelet aggregation and blood coagulation. The ethyl acetate fraction showed the strongest antiplatelet effects (p < 0.05) regardless of agonists used. At 2 mg/mL, percentage inhibition of platelet aggregation of the ethyl acetate fraction was up to 99.5%. Moreover, both ethanol extract and ethyl acetate fraction exhibited anticoagulant activities by prolonging both APTT and PT (p < 0.05), while the n-hexane fraction prolonged APTT (p < 0.05), indicating possible effects on both intrinsic and extrinsic factors involved in the coagulation pathway of aerial PO. This vegetable plant could be a valuable natural source to develop supplementary products or to investigate novel antithrombotic agents for the treatment and prevention of heart diseases.
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