Phytochemical Analysis and Chemical Characterization of Extracts and Blended Mixture of Palm Oil Leaf

doi.org/10.26538/tjnpr/v3i9.2

Authors

  • Patience Onakurhefe Department of Biochemistry, Faculty of Science, Delta State University, Abraka, Nigeria.
  • Fidelis I. Achuba Department of Biochemistry, Faculty of Science, Delta State University, Abraka, Nigeria.
  • Betty O. George Department of Biochemistry, Faculty of Science, Delta State University, Abraka, Nigeria

Keywords:

GCMS,, Oil palm,, Phytochemicals,, Solvents.

Abstract

The importance of herbs and plant- related substances in the management of disease cannot be underestimated. The aim of this study was to determine the phytochemicals in oil palm leaf using various extracting solvents: water, methanol, ethanol, acetone and petroleum ether. Phytochemical analysis was carried out by standard procedure, while chemical composition was determined using GC-MS. The results showed that petroleum ether extract had the highest yield and was closely followed by methanol extract while the least yield was water extract. Phytochemicals analysis of crude oil palm leaves revealed the presence of saponins, steroid, flavonoid, reducing sugar, tannin and alkaloids. From the quantitative phytochemical analysis, reducing sugar had the highest percentage content and this was followed by tannin, alkaloids, cardiac glycoside and flavonoid. Saponin and steroid had the least percentage content. The results also showed that petroleum ether was the best extracting solvent compared to the other solvents used. The GC-MS identified a good number of phytochemicals in oil palm leaf. Thus, we suggest the possibility of utilizing oil palm leaf as a source of herbal remedy.

References

Liu RH. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr. 2003; 78:517S–520S.

Probst YC, Guan VX, Kent K. Dietary phytochemical intake from foods and health outcomes: A systematic review protocol and preliminary scoping. BMJ Open. 2017; 7:e013337.

Elujoba AA, Odeleye, OM, Ogunyemi CM. Traditional medicine development for medical and dental primary health care delivery system in Africa. Afr J Trad Med. 2005; 2(1):46-61.

Aderogba MA, Akinkunmi EO, Mabusela WT. Antioxidant and antimicrobial activities of flavonoid glycosides from Dennettia tripetala G. Baker leaf extract. Nig J Nat Prod Med.2011; 15:49-52.

Lee KW, Bode AM, Dong Z. Molecular targets of phytochemicals for cancer prevention. Nat Rev Cancer. 2011; 11:211.

Chiwuzie J, UKoli F, Okojie O, Isah E, Eriator I. Traditional medicine has come to stay. Trad Med 1987; 8: 240-244.

Chelikani P, Fita I, Loewen P. Diversity of structures and properties among catalases. Cell. Mol. Life Sci. 2004; 61(2):192–208.

Upadhyay S and Dixit M. Role of polyphenols and other phytochemicals on molecular signaling. Oxid Med Cell Longev. 2015; 2015:1-15.

González-Vallinas M, González-Castejón M, Rodríguez- Casado A, Ramírez de Molina A. Dietary phytochemicals in cancer prevention and therapy: A complementary approach with promising perspectives. Nutr Rev. 2013; 71:585–599.

WHO. WHO monographs on selected medicinal plants. 1999; 1:1-295.

Yin NS, Abdullah S, Phin CK. phytochemical constituents from leaves of Elaeis guineensis and their antioxidant and antimicrobial activities. Int J Pharm Pharm Sci. 2013;5:137- 140

Rout SP, Choudary KA, Kar DM, Das L and Jain A. Plants in traditional medicinal system - future source of new drugs. Int J Pharm Pharm Sci. 2009; 1:1-23.

Dauda Z, Hattaa MZM, Awang H. Oil palm Leaf and corn stalk – Mechanical properties and surface characterization. Procedia - Social Behav Sci. 2015; 195: 2047 – 2050.

Abdullah R. An examination of sources of instability in export earnings of Malaysia palm oil. Oil Palm Ind Econ J. 2011; 11(2):1-7.

Sasidharan S, Logeswaran S, Latha, LY. Wound healing activity of Elaeis guineensis leaf extract ointment. Int J Mol Sci. 2012; 13(1):336-347.

Rajoo A, Sasidharan S, Jothy SL, Ramanathan S,Mansor SM. Ultrastructural study of Elaeis guineensis (oil palm) leaf and antimicrobial activity of its methanol extract against Staphylococcus aureus. Trop J Pharm Res. 2013; 12:419-423.

Chonga KH, Zurainia Z, Sasidharanb S, Devib PVK, Lathac LY, Ramanathand S. Antimicrobial activity of Elaeis guineensis Leaf. Pharmacol online. 2008; 3:379-386.

Soundararajan V, Zuraini Z, Yeng C, Lachimanan YL, Jagat RK, Sreenivasan S. The antimicrobial efficacy of Elaeis guineensis: Characterization, in vitro and in vivo studies. Mol. 2012; 17:4860-4877.

Mohamed S. Oil palm leaf: A new functional food ingredient for health and disease prevention. J Food Proc Technol. 2014; 5:300.

Abdul-Razak M. The effects of Oil Palm Fronds (Elaeis guineensis) extract on Lipid Profile and antioxidant enzyme activity in Sprague dawley rats. 2009; MSc Thesis, Universiti Putra Malaysia

Sofowora A. Medicinal plants and traditional medicine in Africa, Spectrum Books Ltd, Ibadan, Nigeria. 1993. 289- 290 p.

Trease GE and Evans WC. Pharmacognosy. 11th Ed, Bailliere Tindall Ltd, London. 1989. 60-75 p.

Harborne JB. Phytochemical Methods, London, Chapman and Hall, Ltd. 1973. 49-188 p.

Dhivya R and Manimegalai K. Preliminary phytochemical screening and GC-MS profiling of ethanolic flower extract of Calotropis gigantean Linn. (Apocynaceae). J Pharmacog Phytochem. 2013; 2(3):28-32.

Felhi S, Daoud A, Hajlaoui H, Mnafgui K, Gharsallah N, Kadri A. Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium elaterium seeds and peels fruits. Food Sci Technol.2017; 37(3):483-492.

Truong D, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC. Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti- inflammatory activities of Severinia buxifolia. J Food Qual. 2019, Article ID 8178294, 9 pages, 2019.

Kannamba B, Winnie TD, Surekha M, Lavanya B. Effect of extraction methods and solvent on phytochemical composition of medicinal plant extracts. Der Pharma Chemica. 2017; 9(9):152-156.

Thouri A, Chahdoura H, Arem AE, Hichri AOH, Hassin RB, Achour L. Effect of solvents extraction on phytochemical components and biological activities of Tunisian date seeds (var. Korkobbi and Arechti). BMC Compl Altern Med. 2017; 17:248.

Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: extraction, isolation, and identification of bioactive compounds from plant extracts. Plants 2017; 6:42.

Mahmood MA, Doughari JH, Chanji FJ. In vitro antibacterial activities of crude extract of Nauclo alantifolia and Daniella oliveri. Sci Res Essays. 2008; 3(3):102-105.

Febrina D, Febriyanti R, Zam SI, Handoko J, Fatah A, Juliantoni J. Antibacterial activity testing and ethanol extract characterization of oil palm fronds (Elaeis guineensis Jacq). Pak J Nutr. 2018; 17:427-433.

Abiola MA, Sarah OO, Osasenaga MI, Okikiola HD, Omotayo MO. Phytochemical, minerals and free radical scavenging profiles of Phoenix dactilyfera L. seed extract. J Taibah Univ Med Sci. 2016; 11(1):1-6.

Moukette BM, Pieme CA, Njimou JR, Nya Biapa, CP, Marco B, Ngogang JY. In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica. Biol Res. 2015; 48:15.

Silva S, Gomes L, Leitão F, Coelho V, Boas L. Phenolic compounds and antioxidant activity of Olea europaea L. fruits and leaves. Food Sci Technol Int. 2006; 12:385-396.

Kumar M, Gupta V, Kumari P, Reddy C, Jha B. Assessment of nutrient composition and antioxidant potential of Caulerpaceae seaweeds. J Food Comp Anal. 2011; 24:270-278.

Ngo TV, Scarlett CJ, Bowyer MC, Ngo PD, Vuong QV. Impact of different extraction solvents on bioactive compounds and antioxidant capacity from the root of Salacia chinensis L.J Food Qual. 2017;Article ID 9305047, 8 pages, https://doi.org/10.1155/2017/9305047.

Dhawan D and Gupta J. Comparison of Different solvents for phytochemical extraction potential from Datura metel plant leaves. Int J Biol Chem. 2017; 11:17-22.

Spink CH and Wyckoff JC. The apparent hydration numbers of alcohols in aqueous solution. J Phys Chem. 1972; 76:1660–1666.

Snehlata K, Sheel R, Kumar B .Evaluation of phytochemicals in polar and non-polar solvent extracts of leaves of Aegle marmelos (L) . IOSR J Biotechnol Biochem. 2018; 4(5):31-38.

Widyawati PS, Budianta TDW, Kusuma FA, Wijaya EL .Difference of Solvent Polarity to phytochemical content and antioxidant activity of Pluchea indicia Less leaves extracts Int J Pharmacog Phytochem Res. 2014-15; 6(4); 850-855.

Nawaz H, Aslam M, Muntaha ST. Effect of solvent polarity and extraction method on phytochemical composition and antioxidant potential of corn silk. Free Rad Antioxid. 2019; 9(1):5-11.

Aziz NA, Halim UN, Abdullah NS. Phytochemical screening and in vitro antibacterial activity of Elaeis guineensis leaves extracts against human pathogenic bacteria. Malay J Anal Sci. 2015; 19(4):775 – 780.

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Published

2019-09-01

How to Cite

Onakurhefe, P., I. Achuba, F., & O. George, B. (2019). Phytochemical Analysis and Chemical Characterization of Extracts and Blended Mixture of Palm Oil Leaf: doi.org/10.26538/tjnpr/v3i9.2. Tropical Journal of Natural Product Research (TJNPR), 3(9), 282–297. Retrieved from https://tjnpr.org/index.php/home/article/view/999