DNA Fingerprinting of Albizia chevalieri (Harms) Mimosoideae Using Ribulose-1, 5- Biphosphate Carboxylase (Rbcl) Gene Sequence
doi.org/10.26538/tjnpr/v3i8.4
Keywords:
Albizia chevalieri, Mimosoideae,, rbcL,, Phylogenetic,, Identification.Abstract
Albizia chevalieri (Harms) Mimosoideae is a tree or shrub of 5-12 m tall that is use in treatment of diabetes mellitus, cancer and has hypoglycaemic effect. Medicinal plant needs to be differentiated in case of those that are substituted or adulterated with other species or varieties that are morphologically or phytochemically indistinguishable. In this study, ribulose-1, 5- biphosphate carboxylase (rbcL) gene sequence profile of A. chevalieri were determined. Our study showed that this specie is phylogenetically related with 98-99% sequence similarities hit with multiple plant species (Albizia amara, Albizia bracteata, Albizia lucidior etc) group on the basis of rbcL gene sequences. The studied specimen and related taxa inferred from partial rbcL gene sequence demonstrated a distinct lineage; therefore, could distinguish the specie as A. chevalieri. It also showed closest similarity that demonstrated the highest level of sequence similarity with A. amara. Therefore, the determined rbcL gene sequence profile from A. chevalieri will improve the identification process with morphology based taxonomic methods.
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