Effects of Ethanol Leaf Extract of Lawsonia inermis Linn. on Carbon Tetrachloride- Induced Liver Injury In Adult Wistar Rats
doi.org/10.26538/tjnpr/v3i8.1
Keywords:
Lawsonia inermis, Wistar rats, , Carbon tetrachloride, phytochemicals, steatosisAbstract
Lawsonia inermis L. (Henna) has been reported to possess hepatoprotective, antimicrobial, antioxidant, antifertility, antiulcer, anticancer and immunostimulatory properties. The effects of ethanol leaf extract of Lawsonia inermis L. on carbon tetrachloride-induced liver injuries were investigated. Phytochemical screening of extract was conducted using established methods. Forty-five (45) adult Wistar rats weighing between 160g – 185g were randomly assigned into nine (9) groups of five (5) animals each. Groups 1-9 received 1mL of distilled water, 0.5mL/kg of olive oil, 250mg/kg of L. inermis, 0.5mL/kg of CCl4 diluted with olive oil at ratio (1:1), 0.5mL/kg of CCl4 diluted with olive oil at ratio (1:1) and standard drug Silymarin at dose 100mg/kg, 0.5mL/kg of CCl4 diluted with olive oil at ratio (1:1) and was then left to recover, 0.5mL/kg of CCl4 diluted with olive oil at ratio (1:1) and was then treated with 500mg/kg of L. inermis, 250mg/kg of L. inermis before receiving 0.5mL/kg of CCl4 diluted with olive oil at ratio (1:1) and 500mg/kg of L. inermis before receiving 0.5mL/kg of CCl4 diluted with olive oil at ratio (1:1) respectively. Administration of ethanol leaf extract of L. inermis not only protected the integrity of plasma membrane of hepatocytes, but also increased the regenerative and reparative capacity of the liver, as shown by the significant reductions in the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and total bilirubin levels. The study demonstrated that the ethanol leaf extract of Lawsonia inermis is able to protect and reverse carbon tetrachloride-induced liver injury in Wistar rats.
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