Biogenic Synthesis and Primary Characterization of Silver Nanoparticles Using Aqueous Extract of Unripe Banana Peel; In Vitro Assessment on Antioxidant and Antibacterial Properties doi.org/10.26538/tjnpr/v4i11.25
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Abstract
The incidence of many life-threatening diseases has been linked to oxidative stress and infectious organisms. The present work investigated the synthesis of silver nanoparticles (AgNPs) using unripe banana peel aqueous extract (UBPE) and assayed for its antioxidant property and antibacterial activity. AgNPs were synthesized from 1 mM AgNO3 solution through the aqueous extract of unripe banana peel. The primary characterization of synthesized silver nanoparticles was done using UV-Visible spectroscopy. The antioxidant and antibacterial properties of the synthesized AgNPs were carried out using standard methods of analysis. The
colourless reaction mixture of the AgNO3 solution and UBPE turned yellowish brown and displayed UV-Visible spectra of 421 nm which is characteristic of silver nanoparticles. The antioxidant assay revealed that the synthesized AgNPs have a total phenol content of 70.64
±0.27 mg GAE/g of extract and showed maximum scavenging activity against DPPH when compared to the standard with IC50 of 59.93 μg/mL. The present study also revealed that the AgNPs prepared from UBPE had an equal zone of inhibition as the standard antibacterial drug used against E.coli but showed a greater zone of inhibition of 37 mm than the standard drug with a zone of inhibition of 31 mm against S. aureus. AgNPs synthesized from UBPE could therefore be utilized in the management of life-threatening diseases caused by oxidative stress and harmful pathogens.
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References
Gabriele P, Natasha I, Mariapaola C, Giovanni P, Federica M, Vincenzo A, Francesco S, Domenica A, Alessandra B. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev. 2017; 2017:1-13.
Krishna D, Chikezie, OM, Yi L. The role of oxidative stress in cancer. Nov ApprCancer Stud. 2020;4(2):350-355.
Lisa MG and James MH. Microbial origins of chronic diseases. Annu Rev Pub Health. 2014; 35:65-82.
Ilaria L, Gennaro R, Francesco C, Giulia B, Luisa A, David D, Gaetano G, Gianluca T, Francesco C, Domenico B, Pasquale A. Oxidative stress, aging, and diseases.ClinInterv Aging. 2018; 13:757–772.
Brian PC. Staphylococcus aureus chronic and relapsing Infections: evidence of a role for persister cells: an Investigation of persister cells, their formation and their role in S. aureusdisease. Bioassays 2014; 36(10):991-996.
Mohammad K.Population structure of gut Escherichia coli and its role in development of extra-intestinal infections. Iran J Microbiol.2010; 2(2):59-72.
Ma W, Jing L, Valladares A, Mehta SL, Wang Z, Andy LP, Bang JJ. Silver nanoparticle exposure induced mitochondrial stress, caspase-3 activation and cell death: amelioration by sodium selemite. Int J Biol Sci. 2015; 11(8):860-867.
Kilcoyne A, Mukesh G, Mahmood U.Prostate cancer Imaging and therapy: potential Role of nanoparticles. J Nucl Med. 2016; 57:105S-110S.
Khan I, Saeed K, Khan I. Nanoparticles: Properties, applications and toxicities. Arab J Chem. 2017;2017:1-24 .
Beyene HD, Werkneh AA, Bezabh HK, Ambaye TG. Synthesis paradigm and applications of silver nanoparticles a review. Sust Mater Technol.2017; 13:18-23.
Fedlheim DL and Foss CA. Metal Nanoparticles. Synthesis, Characterization, and Applications (1st edition). Taylor & Francis group: Books.Google.Com; 2001. 352 p.
Sivakumar SR, Sridhar V, Abdul K. Antioxidant, antimicrobial and sewage treatment of synthesized silver nanoparticles from leaf extract of Hygrophilaauriculata (Schumsch) Heine. IAJPS. 2017; 4(8):2350-2361.
Guilger-Casagrande M and De Lima R. Synthesis of silver nanoparticles mediated by fungi: a review. Front BioengBiotechnol. 2019; 7:287.
Reddy GA, Joy JM, Mitra T, Shabnam S, Shilpa T. Nano silver – areview. Int J Adv Pharm. 2012; 1(2):9-15.
Rajkumar KS, Arun S, Balaji P, Dinesh BM, Thirumurugan R. In vitro antioxidant, antipathogenicity and cytotoxicity effect of Silver Nanoparticles fabricated by Onion (Allium Cepa L.) Peel Extract. Bionanosci.2019; 2019:1-14.
Mohanpuria P, Rana NK, Yadav SK. Biosynthesis of nanoparticles: technological concepts and future Applications. J Nano Res. 2008; 10:507-517.
Kuppusamy P, Ichwan SJ, Al-Zikri PNH, Suriyah WH, Soundharrajan I, Govindan N, Maniam, GP, Yuso MM. In Vitro Anticancer Activity of Au, Ag nanoparticles synthesized using CommelinanudifloraL.aqueous extract against HCT-116 Colon Cancer Cells. Biol Trace Elem Res.2017; 173:297-305.
Saxena A, Tripathi RM, Zafar F, Singh P. Green Synthesis of Silver Nanoparticles using aqueous solution of FicusBenghalensis leaf extract and characterization of their antibacterial activity. Mater Lett.2012; 67:91-94.
Prabhu S andPoulose EK. Silver Nanoparticles: Mechanism of antimicrobial action, synthesis, medical applications, and toxicity Effects. Int Nano Lett. 2012; 2:32.
Margarita IS and Victoria IV. Synthesis of Silver Nanoparticles using orange peel extract prepared by plasmochemical extraction method and degradation of methylene blue under solar irradiation. Adv Mater Sci Eng. 2019; 2019:1-8.
Borase HP, Salunke BK, Salunkhe RB. “Plant extract: a promising biomatrix for ecofriendly, controlled synthesis of Silver Nanoparticles.” Appl Biochem Biotech. 2014; 173(1):1–29.
Kokila T, Ramesh P, Geetha D. A biogenic approach for Green Synthesis of Silver Nanoparticles using peel extract of Citrus Sinensis and Its application. Int J Chem Tech Res.2015; 7(2):804-813.
Kirubha R andAlagumuthu G. Investigation of antibacterial properties of silver nanoparticles using Aerva lanata extract. Am J Pharm Res.2015; 2(3):668-675.
Abhay T andRupa S. Antimicrobial activities of Silver nanoparticles synthesized from peel of fruits and vegetables. JBIRD.2015;1:29-34.
Nooshin A, Gholamreza A, Zahra JA. Green synthesis of silver nanoparticles using Avena sativa L. extract. Nanomed Res J. 2017; 2(1):57-63.
Singleton VL and Rossi, JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid Reagents,” Am J EnolViticult.1965;16:144-158.
Gloria AO, Anthony JA, Emmanuel OA, Samuel WO. Characterization, antibacterial and antioxidant properties of Silver Nanoparticles Synthesized from aqueous extracts of Allium Sativum, Zingiber officinale, and Capsicum frutescens. Pharmacogn Mag. 2017; 13(50):201-208.
Olugbemi TI.Green Synthesis of Silver Nanoparticles from aqueous extract of unripe pawpaw peel and its antimicrobial and antioxidant Property. Fepi-Jopas.2019;1(1):203-209.
Anand KK, Ragini S, Payal S, VirendraBY, Gopal N. Antioxidant and Antibacterial Activity of Silver Nanoparticles Synthesized By Cestrum nocturnum. J Ayurveda Integr Med.2020; 11(1):37-44.
Mahmoudi R, Aghaei S, Salehpour Z, Mousavizadeh A, Khoramrooz SS, Taheripour SM, Bardania H. Antibacterial and antioxidant properties of phyto‐synthesized silver nanoparticles using Lavandula stoechas extract. J Organomet Chem. 2019; E5394:1-9.
Das G, Patra JK, Debnath T, Ansari A, Shin HS. Investigation of antioxidant, antibacterial, antidiabetic, and cytotoxicity potential of Silver Nanoparticles synthesized using the outer peel extract of Anana scomosus (L.). PLoS ONE 2019; 14(8):E0220950.
Zubair AR, Mohammad FH, Nurunnabi M, Shahriar SM, A.J Ahammad S, Shim Y.Y , Martin R, Jae YC. Green chemistry synthesis of Silver Nanoparticles and their Potential anticancer effects. Cancers 2020; 12:855.
Safaepour M, Shahverdi AR, Shahverdi HR, Khorramizadeh MR, Gohari AR. Green synthesis of small silver nanoparticles using Geranoil and its cytotoxicity against Fibrosarcoma Wehi-164. Avicenna J Med Biotechnol. 2009; 1:111-115.
Olugbemi TI. Biosynthesis of Silver Nanoparticles from aqueous extract of unripe plantain peel and its antibacterial assay: A novel biological approach. Int J Pharm Sci Health 2019; 9(5):9-16.
Krithiga N, Rajalakshmi A, Jayachitra A.Green synthesis of Silver Nanoparticles using Leaf Extracts of Clitoriaternatea and Solanumnigrum and study of its antibacterial effect against common nosocomial pathogens. J Nanosci. 2015; 2015:1-8.
Banerjee P, Mantosh S, Aniruddha M, Papita D. Leaf extract mediated green synthesis of Silver Nanoparticles from widely available Indian Plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour Bioproc. 2014; 1(3):1-10.
Hyllested J, Espina PM, Hagen N, Mogensen KB, Kneipp K. Green preparation and spectroscopic characterization of plasmonic Silver Nanoparticles using fruits as reducing Agents. Beilstein J Nanotechnol. 2015; 6:293-299.
Balashanmugam P and Kalaichelvan PT. Biosynthesis characterization of Silver Nanoparticles using Cassia roxburghii DC. Aqueous Extract, and coated on Cotton Cloth for effective antibacterial activity. Int J Nanomed. 2015; 10:87-97.
Aungkana O, Shiv S, Long-Feng W, Rungsinee S, Jong W. One-Step Preparation of Banana Powder/Silver Nanoparticles Composite Films. J Food Sci Technol.2017;54(2):497-506.
Susan KT, Jesteena J, Ragunathan R. Eco-Friendly synthesis of Silver Nanoparticle using Banana (Musa acuminate Colla) Peel, its phytochemical, antimicrobial and anticancer activity. Int J Rec Sci Res. 2017; 8(10):21098- 21106.
Kokila T, Ramesh PS, Geetha D. Biosynthesis of silver nanoparticles from Cavendish banana peel extract and its antibacterial and free radical scavenging assay: a novel biol approach.ApplNanosci.2015; 5:911-920.