Combination of High-Performance Liquid Chromatography (HPLC) Methods for Isolation of Ampelopsin C, a Trimeric Oligostilbene
doi.org/10.26538/tjnpr/v6i4.12
Keywords:
Dipterocarpaceae, Dipterocarpus Semivestitus, Oligostilbenes, Ampelopsin C, ChromatographyAbstract
Ampelopsin C is a trimeric oligostilbene found in a limited number of plant families including Dipterocarpaceae. It shows anti-inflammatory effects in experimental models of psoriasis, induces apoptosis in HepG2 human hepatoma cell line, inhibits human glioma through inducing apoptosis and autophagy and other biological properties. The present work oversees a method for isolation of ampelopsin C from the methanol extract of Dipterocarpus semivestitus leaves. Different chromatographic techniques were employed, including an ultra-high performance liquid chromatography (UHPLC) analysis for method development, a preparative high performance liquid chromatography (prep-HPLC) for isolation and a recycling high performance liquid chromatography (r-HPLC) for purification of the compound. The isolated compound was identified and characterized using nuclear magnetic resonance (NMR). The spectroscopic data confirmed the structure of ampelopsin C.
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