Protective Roles of Annona senegalensis Pers. Extract in N-DiethylnitrosamineInduced Hepatocellular Carcinoma in Male Wistar Rats


  • Omolara F. Yakubu Department of Biochemistry, College of Science and Technology, Covenant University, Canaan Land, Ota PMB 1023, Ogun State, Nigeria
  • Damilohun S. Metibemu Department of Biochemistry, Adekunle Ajasin University, Akungba-Akoko. Ondo State, Nigeria
  • Gbolahan O. Adesina Department of Biochemistry, College of Science and Technology, Covenant University, Canaan Land, Ota PMB 1023, Ogun State, Nigeria


Gene expression, Hepatocellular carcinoma, Annona senegalensis, N-diethylnitrosamine


Hepatic cancer is a common and leading cancer around the world. Preventive measures have been emphasized in view of its prognosis and limited treatment. This study evaluates the protective roles of Annona senegalensis in N-diethylnitrosamine (DEN) – a model of
hepatocarcinoma in male Wistar rats. Rats were intra-peritoneally induced weekly with 100 mg/kg of DEN for 3 weeks and treated with Annona senegalensis extract using a cannula for 21 days. Biochemical assays; aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lipid peroxidation were carried out. Reverse transcription-polymerase chain reactions for the expression of pro-inflammatory RNA, interleukin 6 (IL-6), were equally determined. Annona senegalensis extract (100 mg/kg and 200 mg/kg body weight) significantly (p < 0.05) reduced the activities of ALT and AST in DEN-induced hepatocarcinoma. Lipid peroxidation associated
with the DEN model of the hepatocarcinoma was reversed, and the expression of the proinflammatory, IL-6 mRNA was also down-regulated with Annona senegalensis extract. Histopathological analysis revealed that the treatment (Annona senegalensis extract) restored the liver architecture in DEN model of hepatocarcinoma. Restoration of the liver enzymes, downregulation of the pro-inflammatory, IL-6 mRNA, and restoration of the liver architecture in the DEN model of hepatocarcinoma corroborated the protective potentials of Annona senegalensis extract against hepatocarcinoma. 


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How to Cite

Yakubu, O. F., Metibemu, D. S., & Adesina, G. O. (2020). Protective Roles of Annona senegalensis Pers. Extract in N-DiethylnitrosamineInduced Hepatocellular Carcinoma in Male Wistar Rats: Tropical Journal of Natural Product Research (TJNPR), 4(11), 981–984. Retrieved from

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