Chemical Profiling of Acacia auriculiformis (A. Cunn.) ex Benth. Leaves Extract and its Impact on Gene Expression and Apoptotic DNA Damage in CCl4-Induced Hepatotoxicity in Male Rats doi.org/10.26538/tjnpr/v4i11.16
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Abstract
Acacia auriculiformis (A. Cunn.) ex Benth. is an ornamental evergreen tree with diverse medicinal importance. It was subjected to different chromatographic techniques. Eighteen known phenolics were identified from the leaves using different spectroscopic methods. Among them, twelve compounds were isolated and characterized as: three phenolic acids, one flavone, four flavonols, three isoflavones, and one flavanone. From the LC-ESI-MS profiling technique additional six flavonoids were tentatively identified. HPLC-UV chromatographic quantitation for quercetin 3-O-glucoside, eriodicotyl 7-O-glucoside, genistein and biochanin A was determined as 3.475, 2.406, 6.658 and 5.997 mg/g defatted extract, respectively. The extract genoprotective effect was assessed in 80 adult male rats (10 rats/group) against CCl4-induced genotoxicity in liver tissues (1 mg/kg bw, twice per week), in three doses (35, 70 and 140 mg/kg
bw/d, four weeks), using real-time PCR, comet assay, DNA fragmentation and flow cytometry apoptosis assay. In treated rats, the plant extract significantly down-regulated the hepatic mRNA expression levels of cytochrome P450 (CYP 450) and heat shock protein 70 (Hsp 70) genes and attenuated DNA damage rates to 19.4, 16.2 and 13.8% for the three doses, respectively compared to that of the untreated (23.8%). It also reduced apoptotic DNA fragmentation and apoptotic rates by 26.2, 19.4 and 15.2%, respectively compared to CCl4 treated rats (37.1%). These findings indicated the potential effect of A. auriculiformis leaves to retain DNA structural integrity and its genomic expression against common industrial pollutant such as CCl4 in a dosedependent manner.
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