Comparative HPLC-PDA-MS/MS Tentative Identification of Polyphenolics from the Leaf Extracts of Three Selected Tradescantia species and their In-Vivo Hepatoprotective Activity doi.org/10.26538/tjnpr/v4i11.15
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Abstract
Tradescantia pallida (Rose) D.R. Hunt, T. zebrina Heynh ex. Bosse and T. spathacea Sw. (family Commelinaceae), are three succulent plants widely used in traditional medicine for different purposes. In this study, the phenolic-based phytoconstituents in the leaf ethanol extracts of T. pallida, T. zebrina and T. spathacea were profiled using HPLC-PDA-MS/MS. A total of thirtythree (33) polyphenolic compounds were tentatively identified in all the three plants and were predominantly: flavonoids, phenolic acids, anthocyanins and their glycosides. The total phenolic and total flavonoid contents were estimated colorimetrically as 85.11 ± 0.61, 62.17 ± 1.62 and 35.35 ± 0.14 µg gallic acid equivalent/mg of each dried extract and 29.52 ± 0.12, 11.54 ± 0.01 and 26.87 ± 0.04 µg quercetin equivalent/mg of each dried extract of T. pallida, T. zebrina and T. spathacea, respectively. Oral treatment of rats injected with carbon tetrachloride using the ethanol leaf extracts of the three plants for more than 10 days exhibited significant hepatoprotective activity when compared to silymarin. The crude ethanol extract of T. pallida, having the highest total phenolic and total flavonoid contents displayed the most promising hepatoprotective effect of all the three plants at 200 mg/kg by significantly reducing the serum levels of aspartate aminotransferase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) to 43.9 ± 1.6, 46.8 ± 1.7 and 8.1 ± 0.6, respectively.
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