Cytotoxicity of Butyrospermol and Sitosterone from the Stem Bark of Syzygium aqueum doi.org/10.26538/tjnpr/v4i11.`10
Main Article Content
Abstract
Syzygium aqueum is a public large species of Syzygium genus in Myrtaceae family and it is native to Indonesia and Malaysia. Various parts of S. aqueum have been widely known to be used for traditional medicine, therefore this plant is documented as a medicinal plant. The isolation of some phytochemical compounds from the stem bark of Syzygium aqueum and their cytotoxicity were determined. The extraction and separation of phytochemical compounds in the stem bark of S. aqueum was carried out using standard methods. The structures of isolated compounds were determined by UV, FTIR and NMR. The analysis of all spectra obtained and comparison with literature data led to the conclusion that the isolated compounds were steroids,namely butyrospermol and sitosterone. The cytotoxicity of the isolated compounds was then evaluated against HeLa and T47D cell lines by the MTT method and A549 cell lines by the XTT method.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
References
Barliana MI, Diantini A, Subarnas A, Abdulah R, Izumi T. Inhibition of phosphorylated c - Jun NH (2)-terminal kinase by 2’,4’-dihydroxy-6-methoxy-3,5-dimethylchalcone isolated from Eugenia aquea Burm f. leaves in Jurkat T– cells. Pharmacogn Mag. 2017; 13(Suppl 3):S573-S577.
Kooti W, Servatyari K, Behzadifar M, Asadi-Samani M, Sadeghi F, Nouri B, Marzouni HZ. Effective medicinal plant in cancer treatment, Part 2: Review Study. J. Evid Based Complementary Altern Med. 2017; 22(4):982-995
Greenwell M and Rahman PKSM, Medicinal plants: their use in anticancer treatment. Int J Pharm Sci. 2015; 6(10):4103-4112.
Nahata A. Anticancer agents: a review of relevant information on important herbal drugs. Int J Clin Pharmacol Toxicol. 2017; 6(2):250-255.
Roy A, Attre T, Bharadvaja N. Anticancer agent from medicinal plants: a review. In: Tiezzi A, Karpinski TM (Eds.). New aspects in medicinla plants and pharmacognosy, Poznan, Poland. JBBooks; 2017. 54-73 p.
Hemavathy A, Shanthi P, Sowndharya C, Thiripura SS, Priyadharshni K. Extraction and isolation of bioactive compounds from a therapeutic medicinal plant - Wrightia tinctoria (Roxb.) R. Br Int J Pharmacogn Phytochem Res. 2019; 11(3):199-204.
Tyagi N, Sharma GN, Shrivastava B, Saxena P, Kumar N. Medicinal plants: used in Anti-cancer treatment. Int J Res Dev Pharm Life Sci. 2017; 6(5):2732-2739.
Memon AH, Ismail Z, Al-Suede FSR, Aisha FAF, Hamil MSR, Saeed MAA, Laghari M, Majid AMSA. Isolation, characterization, crystal structure elucidation of two flavanones and simultaneous RP-HPLC determination of five major compounds from Syzygium campanulatum Korth. Molecules. 2015; 20:14212-14233.
Nigam V, Nigam R, Singh A. Distribution and medicinal properties of Syzygium species. Curr Res Pharm Sci. 2012; 02:73-80.
Abera B, Adane L, Mamo F. Phytochemical investigation the root extract of Syzygium guineense and isolation of 2,3,23- trihydroxymethyl oleanate. J Pharmacogn Phytochem. 2018; 7(2):3104-3111.
Mudiana D. Syzygium diversity in Gunung Baung, East Java, Indonesia. Biodversitas. 2016; 17(2):733-740.
Sobeh M, Mahmoud MF, Petruk G, Rezq S, Ashour ML, Youssef FS, El-Shazly AM, Monti DM, Abdel-Naim AB, Wink M. Syzygium aqueum: A Polyphenol-rich leaf Extract exhibits antioxidant, hepatoprotective, pain-killing and antiinflammatory activities in animal models. Front Pharmacol. 2018; 9:1-14.
Manaharana T, Chakravarthi S, Radhakrishnan AK, Palanisamy UD. In vivo toxicity evaluation of a standardized extract of Syzygium aqueum leaf. Toxicol Rep. 2014; 1:718-725.
Palanisamy UD, Ling LT, Manaharan T, Sivapalan V, Subramaniam T, Helme MH, Masilamani T. Standardized extract of Syzygium aqueum: a safe cosmetic ingredient. Int J Cosmet Sci. 2011; 33:269–275.
Manaharan T, Appleton D, Cheng HMC, Palanisamy UD. Flavonoids isolated from Syzygium aqueum leaf extract as potential antihyperglycaemic agents. Food Chem. 2012; 132(4):1802–1807.
Nonaka G, Aiko Y, Aritake K, Nishioka I. Samarangenins A and B, Novel proanthocyanidins with doubly bonded structures, from Syzygium samarangense and S. aqueum. Chem Pharm Bull. 1992; 40(10):2671-2673.
Subarnas A, Diantini A, Abdulah R, Auhrotun A, Hadisaputri YE, Puspitasari IM, Yamazaki C, Kuwano H, Koyama H. Apoptosis induced in MCF-7 human breast cancer cells by 2',4'-dihydroxy-6-methoxy-3,5- dimethylchalcone isolated from Eugenia aquea Burm f. leaves. Oncol Lett. 2015; 9:2303-2306.
Rabeta MS, Chan S, Neda GD, Lam KL, Ong MT. Anticancer effect of underutilized fruits. Int Food Res J. 2013; 20(2):551-556.
Tunjung WAS and Sayekti PR. Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp. [version 2; peer review: 2 approved with reservations]. F1000Research. 2019; 8(168):1-15.
Mutiah R, Listiyana A, Suryadinata A, Annisa R, Hakim A, Anggraini W, Susilowati R. Activity of inhibit the cell cycle and induct apoptosis in HeLa cancer cell with combination of Sabrang onion (Eleutherine palmifolia (L.) Merr) and Starfruit Mistletoe (Macrosolen cochinchinensis (Lour.) Tiegh). J Appl Pharm. 2018; 8(10):122-128.
Amaani R and Dwira S. Phytochemical content an in vitro toxicity of Glycine soja ethanol extract on the A549 lung cancer line cell. IOP Conf. Series: J Phy: Conf Series. 2018; 1073(032042):1-9.
Nair RR, Bhaskar D, Jabbar KK, Amresh N, Sathyamurthy B. Invitro studies on the effect of singiber officinale methanol extract in lung cancer A549 cell lines. World J Pharm Res. 2017; 6(15):1097-1114.
Shettar AK, Madagi SB, Vedamurthy AB. Phytochemical screening and in-vitro antioxidant and antiproliferative activity of aqueous leaf extract of ximtenia americana against non-small cell lung cancer. Cancer Surg. 2018; 3(1):1-8.
Zarea S, Ghaedi M, Miri R, Heiling S, Asadollahi M, Baldwin IT, Jassbi AR. Phytochemical Investigation on Euphorbia macrostegia (Persian wood spurge), Iran J Pharm Res. 2015; 14(1):243-249.
Wu J, Li M, Xiao Z, Zhou Y. Butyrospermol fatty acid esters from the fruit of a Chinese mangrove Xylocarpus granatum. Z. Naturforsch., C, J Biosci. 2006; 61(11):1447- 1449.
Liang XU, Xiao-han WANG, Rui-ya LUO, Shi-qiong LU, Ze-jian GUO, Ming-an WANG, Yang LIU, Li-gang ZHOU. Secondary metabolites of rice sheath blight pathogen Rhizoctonia solani Kühn and their biological activities. J Integr Agric. 2015; 14(1):80–87.
Sriwiriyajan S, Ninpesh T, Sukpondmav Y, Nasomyon T, Graidist P. Cytotoxicity screening of plants of genus Piper in breast cancer cell lines. Trop J Pharm Res. 2014; 13(6):921-928.
Fikroh RA, Matsjeh S, Anwar C. Synthesis and anticancer activity of (E)-2’-hydroxy-2-bromo-4,5-dimethoxychalcone against breast cancer (MCF-7) cell line. Molekul. 2020; 15(1):34-39.
Aslantürk OS. In vitro cytotoxicity and cell viability assays: disadvantages in vitro cytotoxicity and viability assays: principles, advantages and disadvantages, chapter 1; 2018. 4 p.