Phytochemical Screening, Antioxidant and Antibacterial Activities of Ethanol Extract and Fractions of Aleurites moluccana (L.) Willd. Leaves

doi.org/10.26538/tjnpr/v4i11.9

Authors

  • Nia Kristiningrum Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Jember, Jl. Kalimantan I/2, Jember, Indonesia
  • Eka A. Amaliyah Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Jember, Jl. Kalimantan I/2, Jember, Indonesia
  • Dwi K. Pratoko Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Jember, Jl. Kalimantan I/2, Jember, Indonesia

Keywords:

Candlenut, Phytochemical, Antibacterial, Antioxidant, Aleurites moluccana

Abstract

Aleurites moluccana (L.) Willd. (Candlenut) possesses great health benefits and is one of the most widely used plants in Indonesia. The activities of its leaf extract as antioxidant and antibacterial agent is yet to be investigated. Therefore, the present study was aimed at
determining the antioxidant and antibacterial activities of ethanol extract and fractions of candlenut leaves. Samples were extracted using 96% ethanol and multilevel fractionation was done using water, hexane, and ethyl acetate. The crude ethanol leaf extract and the fractions were phytochemically screened to determine bioactive constituents. Antioxidant activity of extract and fractions was determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl reagent) scavenging method, while antibacterial activity was tested against Staphylococcus aureus and Escherichia coli by employing the disc diffusion method. The results of the phytochemical screening revealed the presence of two or more of alkaloid, flavonoid, saponin, triterpenoid, steroid, tannin and polyphenol in each of the ethanol leaf extract and fractions. Antioxidant activity (IC50) test indicated that the ethanol extract, hexane, ethyl acetate and water fractions of candlenut leaves were 141.26, 469.45, 65.68 and 47.91 µg/mL, respectively. The test solutions showed antibacterial activity against both S. aureus and E. coli in the order of water fraction > hexane fraction > ethanol extract > ethyl acetate fraction. The findings from the research suggest the potential use of water fraction of ethanol A. moluccana leaf extract in controlling resistant bacteria. 

References

World Health Organization. World Health Statistics 2014; Italy; 2014. 89-90 p.

Marvibaigi M, Amini N, Supriyanto E, Jamil S, Adibah F, Majid A, Khangholi S. Total phenolic content, antioxidant and antibacterial properties of scurrula ferruginea extracts. J Technol. 2014; 70(5):65-72.

Quintao NLM, Meyre-Silva C, Silva GF, Antonialli CS, Rocha LW, Lucinda-Silva RM, Malheiros A, De Souza MM, Filho VC, Bresolin TMB. Aleurites moluccana (L.) Willd. Leaves: Mechanical Antinociceptive Properties of a Standardized Dried Extract and Its Chemical Markers. EvidBased Compl Altern Med. 2011; 2011:1-10.

Duke JA. Handbook of Medicinal Herbs. Florida: CRC Press USA 1991.

Locher CP, Burch MT, Mower HF, Berestecky J, Davis H, Van Poel B, Lasure A, Vanden Berghe DA, Vlientinck AJ. Anti-microbial activity and anti-complement activity of extracts obtained from selected Hawaiian medicinal plants. J Ethnopharmacol 1995; 49(1):23-32.

Wahyu LNS. Test of inhibitory activity of Ethyl Acetate Extract from Candlenut bark (Aleurites Moluccana L. Willd) on Hela Cancer Cells. Makasar: Alaudin State Islamic University Press 2017.

Mukhriani, Ismail A, Haeria, Syakri S, Fadiyah N. Identification of Antibacterial Groups of Polar and NonPolar Fractions of Candlenut Skins Aleurites Moluccana L. Willd) Using the Contact Bioautography Method. JF FIK UINAM. 2018; 6(1):46-54.

Meyre-Silva C, Mora TC, Biavatti MW, Santos AR, DalMagro J, Yunes RA, Cechinel-Filho V. Preliminary phytochemical and pharmacological studies of Aleurites moluccana leaves [L.] Willd. Phytomed. 1998; 5(2):109-113.

Niazi J, Gupta V, Chakarborty P, Kumar P. AntiInflammatory and Anti-pyretic Activity of Aleuritis moluccana Leaves. Asian J Pharm Clin Res. 2010; 3(1):35- 37.

Pedrosa RC, Meyre-Silva C, Cechinel-Filho V, Benassi JC, Oliveira LFS, Zancanaro V, Dal Magro J, Yunes RA. Hypolipidaemic Activity of Methanol Extract of Aleurites moluccana. Phytother Res. 2002; 16(8):765-768.

Harborne JB. Phytochemical Methods: A Guide to Modern Technique Plant Analysis, Chapman and Hall Publication, London, UK. 1998. 3 p.

Quintao NLM, Rocha LW, Silva GF, Reichert S, Claudino VD, Lucinda-Silva RM, Malheiros A, De Souza MM, Filho VC, Bresolin TMB, Machado MS, Wagner TM, MeyreSilva C. Contribution of α,β-Amyrenone to the AntiInflammatory and Antihypersensitivity E fects of Aleurites moluccana (L.) Willd.. Biomed Res Int. 2014; 2014:1-11.

Meyre-Silva C, Yunes RA, Santos ARS, Dal Magro J, DelleMonache F, Cechinel-Filho V. Isolation of a C glycoside flavonoid with antinociceptive action from Aleurites moluccana leaves. Planta Med. 1999; 65(3):293-294.

Ghosal M and Mandal P. Phytochemical Screening and Antioxidant Activities of Two Selected ‘BIHI’ Fruits Used as Vegetables in Darjeeling Himalaya. Int J Pharm Pharm Sci. 2012; 4(2):567-574.

Mounyr B, Moulay S, Saad KI. Methods for in vitro evaluating antimicrobial activity: A Review. J Pharm Anal. 2016; 6:71-79.

Timothy KH. Basic Microbiology, Prints I. Salatiga: Satya Wacana Christian University 1982.

Katzung BG, Masters SB, Anthony JT. Basic And Clinical Pharmacology. Issue 11. San Francisco: Mc Graw Hill 2009.

Karou D, Savadogo A, Canini A, Yameogo S, Montesano C, Simpore J, Colizzi V, Traore AS. Antibacterial Activity of Alkaloids from Sida acuta. Afr J Biotechnol. 2005; 4(12):1452-1457.

Hendra R, Ahmad S, Sukari A, Shukor MY, Oskoueian E. Flavonoid Analyses and Antimicrobial Activity of Various Parts of Phaleria macrocarpa (Scheff.) Boerl Fruit. Int J Mol Sci. 2011; 12(6):3422-3431.

Madduluri S, Rao KB, Sitaram B. In Vitro Evaluation of Antibacterial Activity of Five Indigenous Plants Extracts Against Five Bacteria Pathogens of Humans. Int J Pharm Pharm Sci. 2013; 5(4):679-684.

Sudira IW, Merdana IM, Wibawa IPAH. Uji Daya Hambat Ekstrak Daun Kedondong (lannea grandis engl) terhadap Pertumbuhan Bakteri Erwinia carotovora. Bull Vet Uday. 2011; 3(1):45-50.

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Published

2020-11-01

How to Cite

Kristiningrum, N., Amaliyah, E. A., & Pratoko, D. K. (2020). Phytochemical Screening, Antioxidant and Antibacterial Activities of Ethanol Extract and Fractions of Aleurites moluccana (L.) Willd. Leaves: doi.org/10.26538/tjnpr/v4i11.9. Tropical Journal of Natural Product Research (TJNPR), 4(11), 895–898. Retrieved from https://tjnpr.org/index.php/home/article/view/955