Aaptos suberitoides Extract Inhibits Cell Migration in Triple Negative Breast Cancer and Decreases NF-κB and MMP-9

doi.org/10.26538/tjnpr/v4i11.13

Authors

  • Hani Andriani Department of Anatomical Pathology, Faculty of Medicine, University Padjadjaran, Bandung, Indonesia
  • Hermin A. Usman Department of Anatomical Pathology, Faculty of Medicine, University Padjadjaran, Bandung, Indonesia
  • Birgitta M. Dewayani Department of Anatomical Pathology, Faculty of Medicine, University Padjadjaran, Bandung, Indonesia
  • Bethy S. Hernowo Department of Anatomical Pathology, Faculty of Medicine, University Padjadjaran, Bandung, Indonesia
  • Muhammad H. Bashari Department of Basic Medical Sciences and Center for Oncology and Stem Cell Studies, Faculty of Medicine, University Padjadjaran, Bandung, Indonesia

Keywords:

F-κB, MMP-9, Migration cells, Aaptos suberitoides

Abstract

Aaptos suberitoides contain aaptamine, which is known to have anticancer properties. This
study aims to analyze the effect of Aaptos suberitoides extract on cell migration, and expression
of NF-κB and MMP-9 in Triple-Negative Breast Cancer (TNBC); MDA-MB-231 cell lines.
Cells were treated with different concentration of extract (0, 1, 2, and 5 ppm) for 24 hours. Cell
Migration was assessed by wound healing/scratch assay. NF-κB and MMP-9 expression were
assessed by immunohistochemistry assay. The results demonstrated that the administration of
Aaptos suberitoides (sea sponge) extract on MDA-MB 231 TNBC cell line caused decrease in
the percentage of the migration area, and reduction of NF-κB and MMP-9 expression,
particularly at highest extract concentration tested; 5 ppm.

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Published

2020-11-01

How to Cite

Andriani, H., Usman, H. A., Dewayani, B. M., Hernowo, B. S., & Bashari, M. H. (2020). Aaptos suberitoides Extract Inhibits Cell Migration in Triple Negative Breast Cancer and Decreases NF-κB and MMP-9: doi.org/10.26538/tjnpr/v4i11.13. Tropical Journal of Natural Product Research (TJNPR), 4(11), 918–921. Retrieved from https://tjnpr.org/index.php/home/article/view/953