Hemostatic and Wound Healing Effects of Gracilaria verrucosa Extract Gel in Albino Rats

doi.org/10.26538/tjnpr/v4i11.12

Authors

  • Rachmi F. Hakim Faculty of Dentistry, Syiah Kuala University, Jl. Tgk. Hasan Krueng Kalee, Kopelma Darussalam, Kec Syiah Kuala, Banda Aceh City, Aceh 23111, Indonesia
  • Fakhrurrazi Fakhrurrazi Faculty of Dentistry, Syiah Kuala University, Jl. Tgk. Hasan Krueng Kalee, Kopelma Darussalam, Kec Syiah Kuala, Banda Aceh City, Aceh 23111, Indonesia
  • Sri Rezeki Faculty of Dentistry, Syiah Kuala University, Jl. Tgk. Hasan Krueng Kalee, Kopelma Darussalam, Kec Syiah Kuala, Banda Aceh City, Aceh 23111, Indonesia
  • Liza M. Sari Faculty of Dentistry, Syiah Kuala University, Jl. Tgk. Hasan Krueng Kalee, Kopelma Darussalam, Kec Syiah Kuala, Banda Aceh City, Aceh 23111, Indonesia
  • Zuhra Marfirah Faculty of Dentistry, Syiah Kuala University, Jl. Tgk. Hasan Krueng Kalee, Kopelma Darussalam, Kec Syiah Kuala, Banda Aceh City, Aceh 23111, Indonesia

Keywords:

Wound healing, Hemostasis, Gracilaria verrucosa, Antioxidant, Albino rats

Abstract

Wound healing in a hostile environment such as oral cavity requires more time. Gracilaria verrucosa, a species of the red algae has been reported to have hemostatic, wound healing, antibacterial and antioxidant potentials. The present study was designed to investigate the effect of G. verrucosa extract gel on oral incised wound healing in rats. G. verrucosa extract and gel were prepared. Phytochemical screening and antioxidant testing of the extract were carried out. Incision wound of 5 mm long in mandibula labial gingiva with a depth reaching the alveolar bone was made on ten albino rats and then divided into 2 groups. G. verrucosa extract gel
(treatment) was applied topically twice a day for 14 days to the wound in the first group, while polyvinylpyrrolidone-sodium hyaluronate gel (control) was applied to the second group. The wounds were observed every day for 14 days. Bleeding time and wound healing potential of test gels were evaluated as well as histological examination of the gingival tissue of the experimental
rats. The results showed that bleeding time for the treatment group was 70 ± 10 s, while the control group was 112.00 ± 8.37 s. The phytochemical screening indicated the presence of flavonoids, alkaloids, phenols, tannins, and saponins in Gracilaria Verrucosa. G. verrucosa extract gel significantly shortened the bleeding time and promoted faster wound healing process, which showed a better fibrillation toward incised oral wound healing in albino rats. Our findings indicated that G. verrucosa extract gel had both hemostatic and wound healing properties. 

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Published

2020-11-01

How to Cite

Hakim, R. F., Fakhrurrazi, F., Rezeki, S., Sari, L. M., & Marfirah, Z. (2020). Hemostatic and Wound Healing Effects of Gracilaria verrucosa Extract Gel in Albino Rats: doi.org/10.26538/tjnpr/v4i11.12. Tropical Journal of Natural Product Research (TJNPR), 4(11), 912–917. Retrieved from https://tjnpr.org/index.php/home/article/view/952