Isolation and Screening of Escherichia coli O157:H7 Bacteriophage


  • Sandra-Emmanuella C. Okafor Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria
  • Daniel Olugbenro Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria
  • Olayemi O. Akinnola Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria
  • Angela O. Eni Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria


Phage therapy, Foodborne disease, Escherichia coli O157:H7, Bacteriophage


The severity of foodborne disease outbreaks caused by Escherichia coli O157:H7 has led to the search for alternative management methods, particularly phage therapy which is currently being revisited. The aim of this study was to isolate and screen lytic E. coli O157:H7 bacteriophages from sewage, for possible use in phage therapy. Sewage sample was collected from the Covenant University sewage treatment plant and enriched with reference E. coli O157:H7, ATCC 10536 for phage isolation. One lytic E. coli O157:H7 bacteriophage was successfully isolated. The isolated bacteriophage was screened using spot assay, to determine its susceptibility to seven previously isolated E. coli isolates. The seven E. coli isolates used in the study were pathotyped using the E. coli O157 latex test kit. The result obtained in this study showed that none of the other E. coli isolates was susceptible to the isolated phage and that none
of the tested isolates belonged to the O157:H7 serogroup. This suggests that the bacteriophage isolated in this study may be specific for E. coli O157:H7. The observed specificity is a useful trait for its use as a potential therapeutic agent for the management of E. coli O157:H7 by phage therapy. Specificity is particularly important given that several harmless E. coli strains that play important roles in maintaining normal intestinal homeostasis, are part of the normal intestinal flora. 


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How to Cite

Okafor, S.-E. C., Olugbenro, D., Akinnola, O. O., & Eni, A. O. (2020). Isolation and Screening of Escherichia coli O157:H7 Bacteriophage: Tropical Journal of Natural Product Research (TJNPR), 4(11), 880–883. Retrieved from