Screening and Optimization of Culture Conditions for Local Production of Tannase from Fungal Species doi.org/10.26538/tjnpr/v4i12.25
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Abstract
Tannase is produced extensively from microorganisms such as fungi, bacteria and yeast as they are diverse naturally. The aim of this study was to isolate, screen and select local microbial strain for the production of tannase as well as optimize culture conditions for the hyper producers. Fifteen fungal species were isolated from decomposed tannin rich materials (soil under Almond leaves undergoing natural deterioration) and its surrounding soil. They were screened with mineral medium compounded with tannic acid. Selected isolates were used for tannase production under solid state fermentation and varied the fermentation conditions. All strains showed various degree of tannic acid utilization as carbon source for enzyme (tannase) production. Three isolates were identified to be potential hyper producers. The fungal species; Aspergillus niger, Penicillum sp. and Aspergillus flavipes yielded the highest enzyme production after incubation. Optimum temperature and pH for tannase production was recorded as 28-45°C and 5.5 respectively. The isolates were good tannase producer with high activities of the enzyme, especially Aspergillus niger, Penicillum sp. and Aspergillus flavipes which gave a high yield of tannase.
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Kumar M, Singh A, Beniwal V, Salar RK. Improved production of tannase by Klebsiella pneumonia using Indian gooseberry leaves under submerged fermentation using Taguchi approach. Appl Microbiol Biotechnol. 2016; 6(46):1–11.
Sharma S, Bhat TK, Dawra RK. A spectrophotometric method for assay of tannase using rhodamine. Anal Biochem. 2000; 279:85-89.
Aguilar CN, Rodriguez R, Gutierrez-Sanchez G, Augur C, Favela-Torres E, Prado-Barragan LA, Ramirez-Coronel A, Contreras-Esquivel JC. Microbial tannases: advances and perspectives. Appl Microbiol Biotechnol. 2007; 76:47–59.
Olutiola PO, Famurewa O, Sonntag HG. An introduction to general microbiology: A Practical Approach. 1st edition. Heidelberg, Germany; 1991. 155 p.
Barnett HL and Hunter BB. Illustrated genera of imperfect fungi. Mineapolis: Burgress Publishing Company, Minneapolis; 1972.
Pinto GA, Leite SG, Terzi SC, Couri S. Selection of tannase producing Aspergillus niger strains. Braz J Microbiol. 2001; 32:24-26.
Yunita AS, Made A, Hasim D. Fractionation and characterization of Tannin Acyl Hydrolase from Aspergillus niger. J Biol Sci. 2009; 16(3):95-99.
Al-Mraai ST, Al-Fekaiki DF, Al-Manhel AJ. Purification and characterization of tannase from the local isolate of Aspergillus niger. J Appl Biol Biotechnol. 2019; 7(01):029-034.
Katyal P and Kaur A. Tannase Production by Fungal Isolates from Tannery Effluent. Chem Sci Rev Lett. 2017; 6(24):2273-2280.
Ajayi AA, Adeniji OF, Egunjobi A, Atolagbe OM, Onibokun EA, Anosike SO, Oniha MI. Isolation and screening of fungal isolates from bambara (vigna subterranea) nuts for tannase production. Afr J Clin Exp Microbiol. 2017; 18(3):167-173.
Aracri FM, Cavalcanti RM, Guimaraes LH. Extracellular Tannase from Aspergillus ochraceus: Influence of the Culture Conditions on Biofilm Formation, Enzyme Production and Application. J Microbiol Biotechnol. 2019; 29(11):1749-1759.
Abdal AK, Al-jubori SS, Muslim SN. Screening, extraction and purification for tannase produced from Iraqi Klebsiella pneumonia isolates and molecular detection of tanA gene. Eurasia J Biosci. 2020; 14:259-263.
Ayesha M, Rida A, Shagufta S, Abrar UlH. Optimized production of tannase and gallic acid from fruit seeds by solid state fermentation. Trop J Pharm Res. 2019; 18(5):911-918.