Screening of Probiotic Characteristics of Lactic Acid Bacteria Isolated from Some Fermented Nigerian Food Products
doi.org/10.26538/tjnpr/v4i12.22
Keywords:
Antibiotic sensitivity, Bile salt-tolerant, Acid tolerant, ProbioticsAbstract
Microorganisms that exert health benefits to the host when consumed in the right proportion are termed Probiotics. Certain lactic acid bacteria, especially Lactobacillus spp. have been considered to be probiotics with proven health benefits and have been used widely in human health management. In this study, in vitro and in vivo evaluation of probiotics characteristics of Lactic acid bacteria (LAB) isolated from traditional Nigerian fermented food products (Ogi and Kunu-zaki) was carried out. Ethical approval was obtained from Covenant Health Research Ethics Committee (CHREC). Ogi and Kunu-zaki were plated on de Mann Rogosa (MRS) agar, and Lactobacillus spp. were isolated and identified by morphology and biochemical characteristics. The probiotic characteristics such as acid and bile salt tolerance, antibiotic susceptibility and haemolytic activity were performed. A total of 15 lactic acid bacteria was isolated. Only 4 (26.7%) of the isolates have high tolerance at pH values 2, 4, and 6.5 for 6 h and hence were reported. The four acid-tolerant isolates showed higher survival rates to 0.1 and 0.3% bile salt for 3 h. All bile and acid tolerant isolates showed different sensitivity to various antibiotics used. Two out of the four probiotics were found to survive the gastrointestinal tract of male albino Wistar rats when they were given a daily dose of 1x109 cfu/mL for two weeks. The four probable Lactobacillus spp. were found to possess characteristics of probiotics and could have the ability to confer health benefits.
References
Hasan MN, Sultan MZ, Mar-E-Um M. Significance of fermented food in nutrition and food science. J Sci Res. 2014; 6:373-386.
O’Bryan C, Crandall P, Ricke S, Ndahetuye J. Lactic acid bacteria (LAB) as antimicrobials in food products: analytical methods and applications. Handbook of Nat Antimicrobial for Food Safety and Quality. 2015; 137-151.
Oranusi SU, Atolagbe OM, Olopade BK. Probiotics in the management of diseases: a review. Int J Curr Res. 2014; 2(8):138-158.
Ohenhen R and Ikenebomeh M. Shelf Stability and Enzyme Activity Studies of Ogi: A Corn Meal Fermented Product. J Amer Sci. 2007; 3:38-42.
Aderiye J, Laleye S, Odeyemi A. Hypolipidemic potential of potential of Lactobacillus and Streptococcus sp from some Nigeria fermented foods. Res J Microbiol. 2007; 2:538-544.
David O and Famurewa O. Prophylactic and bio-therapeutic benefits of `ogi': A lactic acid fermented food. Biol. 2010;2:72-77.
Olukoya D, Ebigwei S, Olasupo N, Ogunjimi A. Production of DogiK: an improved Ogi (Nigerian fermented weaning food) with potentials for use in diarrhoea control. J Trop Pediatr. 1994; 40:108-113.
Odugbemi T, Odujinrin OM, Akitoye CO, Oyerinde JP, Esumeh FI. Study on the pH of ogi, Nigerian fermented weaning food, and its effect on enteropathogenic Escherichia coli, Salmonella typhi and Salmonella paratyphi. J Trop Med Hyg. 1999; 94:219-223.
Ajanaku KO, Ademosun OT, Siyanbola TO, Akinsiku AA, Ajanaku CO, Nwinyi OC. Improving nutritive value of maize-ogi as weaning food using wheat offal addition. Curr Res Nutr Food Sci. 2017; 5(3):206-213.
Gaffa T, Jideani I, Nkama I. Nutritional composition of different types of kunu produced in Bauchi and Gombe states of Nigeria. Int J Food Prop. 2002; 5:351-357.
Chou L and Weimer B. Isolation and characterization of acid- and bile-tolerant isolates from strains of Lactobacillus acidophilus. J Dairy Sci. 1999; 82:23-31.
Chen P, Zhang Q, Dang H, Liu X, Tian F, Zhao J, Chen Y, Zhang H, Chen W. Screening for potential new probiotic based on probiotic properties and ????-glucosidase inhibitory activity. Food Cont. 2014; 35(1):65-72.
Zhang B, Wang Y, Tan Z, Li Z, Jiao Z, Huang Q. Screening of probiotic activities of lactobacilli strains isolated from traditional Tibetan Qula, a raw yak milk cheese. Asian Austral. J anim. 2016; 29:1490-1499.
Mulaw G, Tessema T, Muleta D, Tesfaye A. In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some raditionally Fermented Ethiopian Food Products. Int J Microbiol. 2019; 2019(11):7179514.
Yadav R, Puniya A, Shukla P. Probiotic properties of Lactobacillus plantarum RYPR1 from an indigenous fermented beverage Raabadi. Front Microbiol. 2016; 7:1683.
Shehata MG, El Sohaimy SA, El-Sahn, MA, Youssef MM. Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity. Annals of Agric Sci. 2016; 61(1):65-75.
Olufemi FO, Chijioke OM, Popoola ST, Oluwafemi OF. In vitro study of potential probiotic lactic acid Bacteria isolated from the gut of chickens in Abeokuta, Nigeria. Alex. J Vet Sci. 2018; 58(1):73-84.
Guo X, Kim J, Nam H, Park S, Kim J. Screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties. Anaerobe. 2010; 16:321-326.
Akalu N, Assefa F, Dessalegn A. In vitro evaluation of lactic acid bacteria isolated from traditional fermented Shamita and Kocho for their desirable characteristics as probiotics. Afr J Biotechnol. 2017; 16:594-606.
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