Alpha Amylase Inhibitory and Antioxidant Activities of Leaf Extract and Fractions of Sorindea warneckei Engl. (Anacardiaceae) doi.org/10.26538/tjnpr/v3i2.7

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Published: Feb 1, 2019
Keywords:
Sorindea warneckei,, α-amylase inhibition,, antioxidant,, phenols,, flavonoids,, HPLC analysis.

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Sunday. A. Adesegun
Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria.
Motunrayo V. Badejo
Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria.
Samuel O. Odebunmi
Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria.
Peter D. Ojobo
Central Research Laboratory, College of Medicine, University of Lagos, Nigeria.
Herbert B. Coker
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, Nigeria.

Abstract

Postprandial hyperglycemia is characteristic of type 2 diabetes. The development of alternative medicines containing secondary metabolites with ability to delay or prevent glucose absorption will be a great therapeutic approach in the management of diabetes. The aim of the present study was to evaluate the in-vitro α-amylase inhibitory and antioxidant activities of the leaf extract and
fractions of Sorindea warneckei. The antidiabetic activity of the extract and its fractions were examined by α-amylase bioassay. The antioxidant activity was measured using 2, 2-diphenyl-1- picryhydrazyl (DPPH) radical scavenging and reducing activities. The HPLC analysis, total phenol and total flavonoid contents were also determined. The extract demonstrated substantial concentration-dependent α-amylase inhibitory (IC50 = 400 µg/mL) and DPPH radical scavenging (IC50 = 40 µg/mL) activities. The ethyl acetate fraction showed the highest activity among the fractions with highest phenolic and flavonoid contents. Statistical analysis showed that total phenolic and flavonoid contents had significant (p < 0.05) effect on α-amylase inhibitory and antioxidant activity. HPLC analysis revealed the presence of quercetin, rutin and gallic acid in the active ethyl acetate fraction. This study shows that ethanol leaf extract of Sorindea warneckei exhibits significant α-amylase inhibitory and antioxidant activities which localize mainly in the ethyl acetate fraction. The plant is a veritable source of α-amylase inhibitors.

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How to Cite
A. Adesegun, S., V. Badejo, M., O. Odebunmi, S., D. Ojobo, P., & B. Coker, H. (2019). Alpha Amylase Inhibitory and Antioxidant Activities of Leaf Extract and Fractions of Sorindea warneckei Engl. (Anacardiaceae): doi.org/10.26538/tjnpr/v3i2.7. Tropical Journal of Natural Product Research (TJNPR), 3(2), 58-63. https://tjnpr.org/index.php/home/article/view/881
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Vol. 3 No. 2 (2019): Tropical Journal of Natural Product Research
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

A. Adesegun, S., V. Badejo, M., O. Odebunmi, S., D. Ojobo, P., & B. Coker, H. (2019). Alpha Amylase Inhibitory and Antioxidant Activities of Leaf Extract and Fractions of Sorindea warneckei Engl. (Anacardiaceae): doi.org/10.26538/tjnpr/v3i2.7. Tropical Journal of Natural Product Research (TJNPR), 3(2), 58-63. https://tjnpr.org/index.php/home/article/view/881

References

Bhutkar MA and Bhise SB. Antioxidative effect Tamarindus indica of in alloxan induced diabetic rats. Int J Res Pharm Biomed Sci. 2011; 2:1006-1009.

Go HK, Rahman MM, Kim GB, Na CS, Song CH, Kim JS, Kim SJ, Kang HS. Antidiabetic effects of yam (Discorea batatas) and its constituent allantoin in a rat model of streptozotocin-induced diabetes. Nutr. 2015; 7:8532-8544.

Olokoba AB, Obateru OA, Olokoba LB. Type 2 Diabetes Mellitus: A Review of Current. Oman Med J. 2012; 27(4):269–273.

Karthic K, Kirthiram KS, Sadasivam S, Thayumanavan B, PalvannanT. Identification of α -amylase inhibitors from Syzygium cumini Linn seeds. Ind J Exp Biol. 2008, 46:677-680.

Prashanth D, Padmaja R, Samiulla DS. Effects of certain plant extracts on alpha amylase activity. Fitoterapia 2001; 72:179-181.

Chiasson JL, Josse RG, Gomis R, Haneield M, Karasik A, Laakso M, Stop-NIDDM Trial Research Group. Acarbose for prevention of type 2 diabetes mellitus: the Stop-NIDDM randomized trial. The Lancet 2002; 359:2072-2077.

Bhalodi MS, Shukla S, Saluja AK. In vitro antioxidant activity of the flowers of Ipomea aquatic Forsk. Pharmacogn Mag. 2008; 4:220-226.

Tahrani AA, Piya MK, Kennedy AK, Banett AH. Glycaemic control in type-2 diabetes: targets and new therapies. Pharmacol Ther. 2010; 125:328-331.

Bhandari MR, Anurakkun NJ, Hong G, Kawabata J. Alpha glucosidase and alpha amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergemia ciliata Haw). Food Chem. 2008; 106:247-252.

Wadkar KA, Magdum CS, Patil SS,Naikwade NS. Antidiabetic potential and Indian medicinal plants. J HerbMed Technol. 2008; 2:45-50.

Burkill HM. The useful plants of West Tropical Africa. (2nded., Vol. 1). United Kingdom: Royal Botanical Gardens, Kew, Richmond; 1985. 91 p.

Olawunmi AT, Oluwalana SA, Oyekale KO. Consumers’ preference and phytochemical properties of selected chewing sticks in South-West Nigeria. Acta Satech. 2004; 5:35-41.

Taiwo O, Xu HX, Lee SF. Antibacterial activities of extracts from Nigerian chewing sticks. Phytother Res. 1999; 13:675-679.

Jaiswal YS, Tatke PA, Gabhe SY, Vaidyab AB. Antidiabetic activity of extracts of Anacardium occidentaleLinn. leaves on n-streptozotocin diabetic rats. J Trad Compl Med. 2017; 7(4):421–427.

Roberts LJ, Oates JA, Linton MF, Fazio S, Meador BP, Gross MD, Shyr Y, Morrow JD. The relationship between dose of vitamin E and suppression of oxidative stress in humans. Free Rad Biol Med. 2007; 10:388-393.

Wiernsperger NF. Oxidative stress as a therapeutic target in diabetes: revisiting controversy. Diabet Metab. 2003, 29:579-585.

Sofowora H. Screening Plants for bioactive agents. In Medicinal plants and traditional medicine in Africa. (2nd ed.) Sunshine House, Ibadan, Nigeria: Spectrum Books Ltd; 1993. 134-156p.

Trease GE and Evans WC. A textbook of Pharmacognosy. (13th edition). London: Bailliere Tindall; 1989; 345-365 p.

Kwon YI, Apostolidis E, Shetty K. Evaluation of pepper (Capsicum annum) for management of diabetes and hypertension. J Food Biochem. 2007; 31:370-385.

Adesegun SA, Fajana AI, Orabueze CI, Coker HAB. Evaluation of Antioxidant Properties of Phaulopsis fascisepala C.B.Cl. (Acanthaceae). Evid Based Compl Altern Med. 2009; 6(2):227–231.

Fejes S, Blazovics A, Lugasi A, Lemberkovics E, Petri G, Kery A. In vitro antioxidant activity of Anthricus cerefolium L. (Hoffm) extracts. J Ethnopharmacol. 2000; 69:259-265.

Zou Y, Lu Y, Wei D. Antioxidant activity of a flavonoid rich extract of Hypericum perforatum L. in vitro. J Agric Food Chem. 2004; 52:5032-5039.

Kumar TS, Shanmugam S, Palvannan T, Kumar VMB. Evaluation of antioxidant properties of Elaeocarpus ganitrus Roxb. Leaves. Iran J Pharm Res. 2008; 2:211-215.

Franco OL, Rgden JD, Melo FR, Gosside SMF. Plant αamylase inhibitors and their interaction with insect alphaamylases. Eur J Biochem. 2002; 269:397-412.

Lobo V, Patil A, Phatak A Chandra N. Free radicals, antioxidants and functional food impart on human health. Pharmacogn Rev. 2110; 4:118-126.

Zozulinska D, Wierusz-Wysocka. Type-2 diabetes mellitus as inflammatory disease. Diabet Res Clin Prac. 2006; 74:S12-S16.

Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001; 414:813-820.

Nunes PX, Silva SF, Guedes RJ, Almeida S. Biological oxidations and antioxidants of natural products, phytochemicals as nutraceuticals-Global approaches to their role in nutrition and health. 2012; 1-22 p.

Waheed I, Ahmed M, Syed NH, Ashraf R. Investigation of phytochemical and antioxidant properties of methanol extract and fractions of Ballota linbata (Lamiaceae). Ind J Pharm Sci. 2014; 76:251-256.

Misra S, Pandeya KB, Tiwari Ak, Ali AZ, Saradamani T, Agawane SB, Madhusudana K. Antihyperglycemic, alphaglucosidase inhibitory and DPPH radical scavenging activity of 5-bromosalicyladehyde and Schiff bases. Med Chem Res. 2010; 3:1-7.

Floris AL, Peter LL, Peinier PA, Bov HL, Guy BR, Chris W. Glucosidase inhibitors for patients with type 2 diabetes. Diabet Care 2005; 28:154-163.

Alterio AA, Fava DAF, Navarro F. Interaction of the daily ingestion of green tea (Camellia sinesis) in the cellular metabolism and adipose cell promoting emagredimento. Rev Brs Obes Nut Emag. 2007; 1:27-37.

Ghorbani A. Mecanisms of antidiabetic effects of flavonoid rutin. Biomed Pharmacother. 2017; 96:305-312.

Huang TH, Peng C, Kota BP, Li CQ, Yamahara J, Roulfogalis BD. Antidiabetic action of Punica granatum flower extract: activation of PPAR gamma and identification of an active component. Toxicol Appl Pharmacol, 2005; 207:160-169.

Yang DK and Kang HS. Antidiabetic effect of cotreatment with quercetin and resveratrol in streptozotocin-induced diabetic rats. Biomol Ther. 2018; 26:130-138