Phytochemical Analysis and Antibacterial Activity of Trema orientalis (Ulmaceae) Stem Bark Extracts on Respiratory Tract Bacteria doi.org/10.26538/tjnpr/v2i12.4
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Abstract
Respiratory tract infection causing micro-organisms are becoming increasingly resistant to common antibiotics. This negative health trend has rekindled interest in the search for plant constituents as sources of antibacterial agents that can be used for the treatment of respiratory tract infectious diseases. The objective of this study is to carry out phytochemical analysis and antibacterial activity of Trema orientalis stem bark extracts on respiratory tract bacteria. Phytochemical screening and thin layer chromatography (TLC) were done on T. orientalis stem bark extracts obtained by successive extraction with n-hexane, chloroform, ethyl acetate, methanol
and water. Also, the antibacterial activity of the extracts were carried out using clinical isolate of Pseudomonas fluorescens, Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, and Proteus mirabilis. The most active chloroform extract was further screened by TLC bioautography using the test microorganisms. The phytochemical screening of the stem bark extracts revealed the presence of flavonoids, steroids, terpenoids, saponins and tannins. The chloroform extract inhibited the growth of all the test organisms especially E. coli and K. pneumonia. The minimum inhibitory concentration (MIC) for E. coli and K. pneumonia were 0.4 mg/mL and 1.6 mg/mL respectively; while the minimum bactericidal concentration (MBC) was 0.8 mg/mL for E. coli and 12.8 mg/mL for K. pneumonia. This study has justified the traditional use of this plant for the treatment of respiratory tract infections
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Tuo K, Beourou S, Toure AO, Ouattara K, Meite S, Ako AAB, Yao SS, Koffi D, Coulibay B, Coulibaly A, Djaman AJ. Antioxidant activities and estimation of the phenols and flavonoids content in the extracts of medicinal plants used to treat malaria in Ivory Coast. Int J Curr Microbiol Appl Sci. 2015; 4 (1):862-874.
Mahesh B and Satish S. Antimicrobial Activity of Some Important Medicinal Plant against Plant and Human Pathogens. World J
Agric Sci. 2008; 4(S):839-843.
Arora DS and Kaur GJ. Antibacterial activity of some Indian Medicinal plants. J Nat Med. 2007; 61(3):313-317.
Anon K. Ethanobotany in the search for new drugs. Ciba Foundation Syposium, John Wiley and Sons: New York; 1994. 188 p.
Rastogi RP and Mehrotra BN. Compendium of Indian Medicinal Plants Vol. 2. New Delhi, India: Central Drug Research Institute, Lucknow Publications and Information Directorate; 1993. 169–170 p.
Adinortey MB, Galyuon IK, Asamoah NO. Trema orientalis Linn. Blume: A potential for prospecting for drugs for various uses. Pharmacogn Rev. 2013; 7(13):67–72.
Orwa C, Mutua A, Kindt R, Jamnadass R, Simons A. “Agroforestry database: A tree reference and selection guide version 4.0,” Url: http://www.Worldagroforestry.org/af/treedb/ 2009; (Accessed on 15 February 2015)
Watt JM and Breyer-Brandwijk MG. The Medicinal and Poisonous Plants of Southern and Eastern Africa; (2nd ed.). London: Livingstone; 1962. 982 –987 p.
Iwu M. Handbook of African Medicinal Plants. (1st Ed) CRC Press, Boca Raton: Florida; 1993. 251–252 p.
World Health Organisation (WHO) African region – Communicable disease prevention, control and eradication. 2007
https://www.who.int/countries/eth/areas/cds/en/
WHO, “Country Health System Fact Sheet: Nigeria,” WHO, Geneva, Switzerland, 2006, http://www.afro.who.int/home/countries/fact_sheets/nigeria.pdf.
Harborne JB. Phytochemical methods - a guide to modern techniques of plant analysis. 2nd ed. London: Chapman and Hall; 1984. 4–16 p.
Clinical and Laboratory Standards Institute (CLSI) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard (9th Ed.) 2012; 32 (2): 13 – 19.
Choma IM and Jesionek W. TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants. Chromatography 2015; 2:225-238.
Chowdhury AA and Islam MS. Antibacterial activity of Trema orientalis. Dhaka Univ. J Pharm Sci. 2004; 3(1 - 2):115–117.
Jayashree R, Albert LS, Minaram N, Mahuya S. Antibacterial Efficacy of Bark extracts of an Ethnomedicinal plant Trema orientalis Blume. Curr Trends Biotechnol Pharm. 2012; 6 (4):464-471.
Fabry W, Okemo PO, Ansorg R. Antibacterial activity of East African medicinal plants. J Ethnopharmacol. 1998; 60:79-84.
Haas W, Pillar CM, Hesje CK, Sanfilipo CM, Morris TW. Bactericidal activity of Besifloxacin against Staphylococci, Streptococcus pneumoniae and Haemophilus influenza. JAntimicrob Chemother. 2010; 65(7):1441-1447.
Choma IM and Grzelak EM. Bioautography detection in thin-layer chromatography. J Chromatography A: 2011; 1218:2684 – 2691.