Antibacterial Activity of a Triterpenoid from n-Butanol Extract of the Root Bark of Ficus sycomorus (Linn) doi.org/10.26538/tjnpr/v2i10.3
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Abstract
Microorganisms have developed and are still developing resistance to most antibacterial agents in use. Thus, there is a need to search for new drugs that are of organic origin from medicinal plants, because they serve as a primary source of new medicine and lead compounds for the development of new antibacterial agents. The aim of this study was to isolate, characterise and investigate the antibacterial activity of lupeol from the root bark of Ficus sycomorus. Phytochemical investigation of n-butanol extract from the root bark of Ficus sycomorus showed the occurrence of steroids, triterpenes, flavonoids, alkaloids, and tannins following microwave-assisted extraction. A triterpenoid compound, lupeol, was isolated from the root bark of the plant. The isolated compound was screened for antibacterial activity against Escherichia coli, Samonella typhi, Bacillus subtilis and Staphylococcus aureus using agar well diffusion method. The isolated compound exhibited antibacterial activity against some of the tested microorganisms; B. subtilis and E. coli. This showed that the compound may serve as a potential source of antibacterial agent
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Bwalya AG. Evaluation of the in vitro biological activities and phytochemical profiling of eight Ficus species collected in Zambia (Doctoral dissertation, UCL (University College London).
Ogbuewu IP, Okechukwu KC, Emenalom OO, Okoli IC. Diversity of plants used in animal reproduction in Enugu State Nigeria. Glob. J Anim Sci Res. 2015; 3:234-238.
Zumbes HJ, Mawak JD, Ekpiwre DV, Babalola OB, Gokir JD, Dabo AD. Antibacterial Activities of Leaf and Stem Bark Extracts of Ficus sycomorus on Clinical Isolates of Salmonella Species and Its In vivo Toxicity Against Swiss Albino Mice. Ind J Appl Res. 2015; 5(10):527-530.
Abubakar US, Danmalam UH, Musa KY, Banni Z, Yahaya I, Abba A, Sani A. Phytochemical and Antimicrobial Screening of Methanol Root Bark Extract of Ficus sycomorus Linn.(Moraceae). Nig J Pharm Sci. 2015; 14(2):1-7.
Paré JJ, Bélanger JM, Stafford SS. Microwave-assisted process (MAP™): a new tool for the analytical laboratory. TrAC Trend Anal Chem. 1994; 13(4):176 - 184.
Patil PS and Shettigar R. An advancement of analytical techniques in herbal research. J Adv Sci Res. 2010; 1(1):08 -14.
Harborne AJ. Phytochemical methods a guide to modern techniques of plant analysis. Springer science and business media.1998: 5 - 7.
El-Sayed MM, Mahmoud MA, El-Nahas HA, El-Toumy SA, El-Wakil EA, Ghareeb MA. Bio-guided isolation and structure elucidation of antioxidant compounds from the leaves of Ficus sycomorus. Pharmacologyonline. 2010; 3:317 - 332.
Sandabe UK, Onyeyili PA, Chibuzo GA. Phytochemical screening and effect of aqueous extract of Ficus sycomorusL. (Moraceae) stembark on muscular activity in laboratory animals. J Ethnopharmacol. 2006; 104(1 - 2):283-285.
Mudi SY, Muhammad A, Musa J, Datti Y. Phytochemical Screening and Antimicrobial Activity of Leaves and Fruits Extract of Ficus sycomorus. ChemSearch J. 2015; 6(1):62 -67.
Muktar B, Bello IA, Sallau MS. Isolation, characterization and antimicrobial study of lupeol acetate from the root bark of Fig - Mulberry Sycamore (Ficus sycomorus LINN). J Appl Sci Environ Manag. 2018; 22(7):1129 - 1133.
Evans WC. Trease and evans. WB Saunders Harcourt Publishers Ltd. 2002; 292:357 – 375 p.
Silva GL, Lee IS, Kinghorn AD. Special problems with the extraction of plants. In Natural Products Isolation 1998 (pp. 343 - 363). Humana press.
Sofowora A. Medicinal plants and traditional medicine in Africa. John Wiley and sons LTD; 1982.
National Committee for Clinical Laboratory Standards (United States). Performance standards for antimicrobial disk susceptibility tests. National Committee for Clinical Laboratory Standards; 2002.
Vollekova A, Košťálová D, Sochorova R. Isoquinoline alkaloids from Mahonia aquifolium stem bark are active against Malassezia spp. Folia microbiologica. 2001; 46(2):107.
Jamal AK, Yaacob WA, Din LB. A chemical study on Phyllanthus reticulatus. J Phy Sci. 2008; 19(2):45 - 50.
Silverstein RM, Webster FX, Kiemle DJ, Bryce DL. Spectrometric identification of organic compounds. John wiley and sons; 2014 Sep 29.
Sutomo S, Rianto S, Setyowati EP. Isolation and identification of active compound of n - hexane fraction from Kasturi (Mangifera casturi Konsterm) against antioxidant and immunomodulatory activity. J Biol Sci. 2013; 13(7):596 - 604.
Suryati S, Nurdin H, Dachriyanus D, Lajis MN. Structure elucidation of antibacterial compound from Ficus deltoidea Jack leaves. Indon J Chem. 2011; 11(1):67 - 70.
Saleem M. Lupeol, a novel anti-inflammatory and anticancer dietary triterpene. Cancer letters. 2009; 285(2):109 -115.
Wächter GA, Valcic SU, Flagg ML, Franzblau SG, Montenegro G, Suarez EN, Timmermann BN. Antitubercular activity of pentacyclic triterpenoids from plants of Argentina and Chile. Phytomed. 1999; 6(5):341 -345.
Ray CG and Ryan KJ. Enteroviruses. Sherris Medical Microbiology, 4th Edition. Ed. Ryan KJ, Ray CG. The McGraw - Hill Companies. 2004:531 - 541.
Atlas RM. Handbook of microbiological media. CRC press; 2010 Mar 17.