Evaluation of the Antiglycation Effect of Branched Chain Amino Acids and Phytochemical Compounds on RAW 264.7 Cell Line and their Synergistic Effect on Colorectal Cancer Cell Line Panel

doi.org/10.26538/tjnpr/v5i1.11

Authors

  • Moath Alqaraleh Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan
  • Violet Kasabri Department of Pharmacy, Faculty of Pharmacy. The University of Jordan, Amman, Jordan
  • Ahmad Aljaafreh Department of Medical Analysis, Mutah University, Mutah, Karak, 61710, Jordan
  • Ibrahim Al-Majali Department of Medical Analysis, Mutah University, Mutah, Karak, 61710, Jordan
  • Nihad Al-Othman Division of Anatomy, Biochemistry and, Genetics. Faculty of Medicine and Health Sciences, An-Najah National University, Nablus
  • Khaled Khleifat Department of Biology, Mutah University, Karak, Mutah, 61710 Jordan
  • Sara Khaleel Faculty of Pharmacy, Zarqa University, P.O. Box 132222, Zarqa, 13132, Jordan
  • Nafe M. Al‐Tawarah Department of Medical Analysis, Mutah University, Mutah, Karak, 61710, Jordan
  • Haitham Qaralleh Department of Medical Analysis, Mutah University, Mutah, Karak, 61710, Jordan
  • Mohammad Al-majali Department of Biological Sciences, Faculty of Science. The University of Jordan, Amman, Jordan
  • Israa Yousef Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan
  • Arwa Al-Khatib Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan
  • Alia S. Khwaldeh Department of Medical Laboratory Sciences, Faculty of Pharmacy, Jadara University, Irbid, Jordan

Keywords:

Flavonoids, BCAAs, Antiglycation, Cytotoxicity, Co-incubation

Abstract

Flavonoids and branched chain amino acids (BCAAs) have been recognized as attractive agents for research due to their role in diabetes and cancer. The present study investigates the antiglycation effect of BCAAs and flavonoid compounds and their cytotoxic effect on different colorectal cancer cell lines individually and in combination. This study was conducted by using RAW 264.7 cell line (murine monocyte-macrophage cell and Methylglyoxal (MGO) for inducing the glycation effect. The Sulforhodamine B (SRB) assay was used to study the cytotoxicity of BCAAs and flavonoids compounds using colorectal cell lines SW480, SW620 and CACO2. Flavonoids showed significant antiglycation effect compared to Aminoguanidine (AMG), while none of the BCAAs compounds displayed antiglycation effect. All flavonoids and branched chain amino acids showed less degree of cytotoxicity against SW620, CACO2 and SW480 as compared to cisplatin. The synergetic effects was only shown against SW620 and CACO2 cell lines by using the combination of Apigenin and BCAAs while antagonistic effect appeared when combination of Quercetin and Hesperidin were used against SW480, SW620 and CACO2 cell lines. Flavonoids such as Quercetin, Apigenin and Hesperidin could be used as a remedy for diabetes due to their surpassing effect upon antiglycation action of AMG. 

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Published

2021-01-01

How to Cite

Alqaraleh, M., Kasabri, V., Aljaafreh, A., Al-Majali, I., Al-Othman, N., Khleifat, K., … Khwaldeh, A. S. (2021). Evaluation of the Antiglycation Effect of Branched Chain Amino Acids and Phytochemical Compounds on RAW 264.7 Cell Line and their Synergistic Effect on Colorectal Cancer Cell Line Panel: doi.org/10.26538/tjnpr/v5i1.11. Tropical Journal of Natural Product Research (TJNPR), 5(1), 88–93. Retrieved from https://tjnpr.org/index.php/home/article/view/827

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