Green Synthesis of Silver Nanoparticles using Aqueous Leaf Extract of Ocimum basilicum and Investigation of their Potential Antibacterial Activity
doi.org/10.26538/tjnpr/v5i1.12
Abstract
Bacterial resistance to antibiotics has emerged as a common medical problem that requires
urgent attention. Thus, investigation on seeking for a better alternative is a paramount issue.
Recently, silver nanoparticles (SNPs) have shown promising results as bactericidal agent against
both gram positive and gram negative bacteria without cytotoxicity. The objectives of this
study were to synthesize SNPs using Ocimum basilicum leaf extract, characterize the
nanoparticles and investigate their antibacterial activity. SNPs were prepared by mixing AgNO3
precursor solution with the leaf extract of O. basilicum. The biologically synthesized SNPs were
characterized using UV-Visible spectral, transmission electron microscopic and fourier
transform infrared spectroscopic analyses. Bactericidal efficiency of the biologically synthesized
SNPs was examined against certain bacteria known for their resistance against conventional
antibiotics. The results indicated that the synthesized SNPs were crystalline, spherical in nature
and their size ranged between 18.7-26.2 nm. Also, the antibacterial sensitivity testing showed a
great inhibitory effect of SNPs against the test bacterial pathogens which included Proteus
vulgaris, Escherichia coli, Klebsiella pneumonia and Bacillus sp. Our finding suggest that
biologically synthesized SNPs using aqueous leaf extract of O. bascilicum has a promising
antimicrobial activity against some pathogenic bacteria with proven resistance to conventional
antibiotics.
Keywords: Silver nanoparticles, Ocimum basilicum, Extract, Bacterial resistance.
References
Siddiqi KS and Husen A. Recent advances in plantmediated engineered gold nanoparticles and their application in biological system. J Trac Elem Med Biol.2017; 40:10-23.
Prasannaraj G and Venkatachalam P. Green engineering of biomolecule-coated metallic silver nanoparticles and their
potential cytotoxic activity against cancer cell lines. Adv Nat Sci Nanosci Nanotechnol. 2017; 8(2):025001.
Cai R, Du Y, Yang D, Jia G, Zhu B, Chen B and Yang L. Free-standing 2D nanorafts by assembly of 1D nanorods for biomolecule sensing. Nanoscale 2019; 11(25):12169-12176.
Reddy NR, Bhargav U, Kumari MM, Cheralathan KK, Shankar MV, Reddy KR, Saleh TA, Aminabhavi TM. Highly efficient solar light-driven photocatalytic hydrogen production over Cu/FCNT stitania quantum dots-based heterostructures, J Environ Manage. 2020;15(254):1097617.
Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS. Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids and Surfaces B: Biointerfaces. 2009; 73(2):332-338.
Zheng H, and Wang L. Banana peel carbon that containing functional groups applied to the selective adsorption of Au
(III) from waste printed circuit boards. Soft Nanosci Letters. 2013; 3:29-36.
Mittal J, Batra A, Singh A, Sharma MM. Advances in Natural Sciences. Nanosci Nanotechnol. 2014; 5:043002.
Ghaedi M, Yousefinejad M, Safarpoor M, Khafri HZ, Purkait MK. Rosmarinus officinalis leaf extract mediated green synthesis of silver nanoparticles and investigation of its antimicrobial properties. J Indust Engr Chem. 2015; 31:167-172.
Sadeghi B and Gholamhoseinpoor F. A study on the stability and green synthesis of silver nanoparticles using Ziziphora tenuior (Zt) extract at room temperature. Spectrochimica Acta Part A: Mol Biomol Spectrosc. 2015; 134:310-315.
Gomathi M, Rajkumar P, Prakasam A, Ravichandran K. Green synthesis of silver nanoparticles using Datura stramonium leaf extract and assessment of their antibacterial activity. Res-Efficient Tech 2017; 3:280-284.
Judith VJ, Jayaprakash N, Kombaiah K, Kaviyarasu K,John KL, Jothi R, Hamad A, Al Lohedan, Ali VM, Maaza M. Bioreduction potentials of dried root of Zingiber officinale for a simple green synthesis of silver nanoparticles: Antibacterial studies. J Photochemi Photobiol Biol. 2017; 177:62-68.
Krishnadhas L, Santhi R, Annapurani S. Green synthesis of silver nanoparticles from the leaf extract of Volkameria
inermis. Inter J Pharm Clin Res. 2017; 9(8):610-616.
Sivaranjani KM and Meenakshisundaram M. Biological synthesis of silver nanoparticles using Ocimum basillicum
leaf extract and their antimicrobial activity. Int Res J Pharm. 2013; 4:225-229.
Mittal AK, Chisti Y, Banerjee UC. Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv 2013; 31(2):346-356.
Forbes BA, Sahm DF, Weissfeld AS. Baily and Scott's Diagnostic Microbiology. Mosby (Elsevier). USA. 2007; 12:171-178 p.
Cockerill FR, Wikler MA, Alder J, Dudley MN, Eliopoulos GM, Ferraro MJ, Jean B, Michael N, George M, Powell M.Performance standards for antimicrobial susceptibility testing: twenty-second informational supplement. Clin Lab
Standards Institute. 2012; 32(3):M100-S22.
Varaldo PE. Antimicrobial resistance and susceptibility testing: an evergreen topic. J Antimicrob Chemother. 2002;
(1):1-4.
Nagati V, Rama K, Rajkiran B, Jahnavi A, Manisha RD, Pratap R, and Manthur P. Green synthesis of plant-mediated
silver nanoparticles using Withania somnifera leaf extract and evaluation of their antimicrobial activity. Int J Adv Res. 2013; 1(9):307-313.
Ahmed AA, Hamzah HM, Maaroof MN. Analysing formation of silver nanoparticles from the filamentous fungus Fusarium oxysporum and their antimicrobial activity. Turk J Biol. 2018; 42:54-62.
Wang Q, Larese-Casanova P, Webster TJ. Inhibition of various gram-positive and gram-negative bacteria growth
on selenium nanoparticle coated paper towels. Int J Nanomed. 2015; 10:2885-2894.
Rajesha S, Dharanishanthib V, VinothKannac A. Antibacterial mechanism of biogenic silver nanoparticles of
Lactobacillus acidophilus. J Exp Nanosci. 2015; 10(15):1143-1152.
Malapermal V, Botha I, Krishna SBN, Mbatha JN. Enhancing antidiabetic and antimicrobial performance of Ocimum basilicum, and Ocimum sanctum (L.) using silver nanoparticles. Saudi J Biolog Sci. 2017; 24(6):1294-1305.
Das M, and Smita SS. Biosynthesis of silver nanoparticles using bark extracts of Butea monosperma (Lam.) Taub. and
study of their antimicrobial activity. Appl Nanosci. 2018; 8(5):1059-1067.
Femi-Adepoju AG, Dada AO, Otun KO, Adepoju AO, Fatoba OP. Green synthesis of silver nanoparticles using terrestrial fern (Gleichenia Pectinata [Willd.] C. Presl.): Characterization and antimicrobial studies. Heliyon 2019; 5(4):e01543.
Dada AO, Adekola FA, Adelani-Akande T, Oluwasesan BM. Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary
antibacterial testing. Heliyon 2019; 5(10):e02517.
Elumalai D, Sathiyaraj M, Vimalkumar E, Kaleena PK, Hemavathi M, Venkatesh, P. Bio fabricated of silver nanoparticles using Ocimum basilicum and its efficacy of antimicrobial and antioxidant activity. Asian J Green Chem. 2019; 3:103-124.
Pirtarighat S, Ghannadnia M, and Baghshahi S. Biosynthesis of silver nanoparticles using Ocimum basilicum cultured under controlled conditions for bactericidal application. Mater Sci Engr. 2019; 98:250-255.
Dalir SJ, Djahaniani H, Nabati F, Hekmati M. Characterization and the evaluation of antimicrobial activities of silver nanoparticles biosynthesized from Carya illinoinensis leaf extract. Heliyon 2020; 6(3):e03624.
Dada AO, Inyinbor AA, Idu EI, Bello OM, Oluyori AP, Adelani-Akande TA, Okunola AA, Dada O. Effect of operational parameters, characterization and antibacterial studies of green synthesis of silver nanoparticles using Tithonia diversifolia. Peer J. 2018; 30(6):e5865.
Bagherzade G, Tavakoli MM, Namaei MH. Green synthesis of silver nanoparticles using aqueous extract of saffron (Crocus sativus L.) wastages and its antibacterial activity against six bacteria. Asian Pac J Trop Biomed. 2017; 7(3):227-233.
Rathi SPR, Reka M, Poovazhagi R, Arul KM, Murugesan K. Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam. Spectrochim Acta Mol Biomol Spectrosc. 2015; 135:1137-1144.
Du Y, Huang Z, Wu S, Xiong K, Zhang X, Zheng B, Nadimicherla R, Fu R, Wu D. Preparation of versatile yolk-shell anoparticles with a precious metal yolk and a microporous polymer shell for high-performance catalysts and antibacterial agents. Polymer. 2018; 137:195-200.
Muthulakshmi K, Uma C, Sivagurunathan P, Kumar SS, Yoganathan K. Evaluation of antimicrobial activity of silver nanoparticles from silver resistant isolate Enterobacter cloacae (MK163462) against certain clinical. Int J Pharm
Bio Sci. 2018; 8(4):558-564.
Xu SY and Pan SY. The failure of animal models of neuroprotection in acute ischemic stroke to translate to clinical efficacy. Med Sci Monitor Basic Res. 2013; 19(37):3-110.
Karthiga P. Preparation of silver nanoparticles by Garcinia mangostana stem extract and investigation of the antimicrobial properties. Biotechnol Res Innova. 2018; 2(1):30-36.
Franci G, Falanga A, Galdiero S, Palomba L, Rai M, Morelli G, Galdiero M. Silver nanoparticles as potential antibacterial agents. Molecules. 2015; 20(5):8856-8874.
Ajitha B, Reddy YAK, Reddy PS. Biogenic nano-scale silver particles by Tephrosia purpurea leaf extract and their
inborn antimicrobial activity. Spectrochimica Acta Part A: Mol Biomol Spectroscop. 2014; 121:164-172.
Muhsin TM and Hachim AK. Mycosynthesis and characterization of silver nanoparticles and their activity against some human pathogenic bacteria. World J Microbiol Biotechnol. 2014; 30(7):2081-2090.
Umashankari J, Inbakandan D, Ajithkumar TT, Balasubramanian T. Mangrove plant, Rhizophora mucronata (Lamk, 1804) mediated one pot green synthesis of silver nanoparticles and its antibacterial activity against aquatic pathogens. Aquatic Biosystems. 2012; 8(1):1-7.
Hajipour MJ, Fromm KM, Ashkarran AA, de Aberasturi DJ, de Larramendi IR, Rojo T, Mahmoudi M. Antibacterial properties of nanoparticles. Trends in Biotechnol. 2012; 30(10):499-511.
Saleh TH, Hashim ST, Malik SN, Al-Rubaii BL. Downregulation of flil gene expression by Ag nanoparticles and TiO2 nanoparticles in pragmatic clinical isolates of Proteus mirabilis and Proteus vulgaris from urinary tract infection. Nano Biomed Eng, 2019; 11(4):321-332.
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