Green Synthesis of Silver Nanoparticles using Aqueous Leaf Extract of Ocimum basilicum and Investigation of their Potential Antibacterial Activity doi.org/10.26538/tjnpr/v5i1.12
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Abstract
Bacterial resistance to antibiotics has emerged as a common medical problem that requires
urgent attention. Thus, investigation on seeking for a better alternative is a paramount issue.
Recently, silver nanoparticles (SNPs) have shown promising results as bactericidal agent against
both gram positive and gram negative bacteria without cytotoxicity. The objectives of this
study were to synthesize SNPs using Ocimum basilicum leaf extract, characterize the
nanoparticles and investigate their antibacterial activity. SNPs were prepared by mixing AgNO3
precursor solution with the leaf extract of O. basilicum. The biologically synthesized SNPs were
characterized using UV-Visible spectral, transmission electron microscopic and fourier
transform infrared spectroscopic analyses. Bactericidal efficiency of the biologically synthesized
SNPs was examined against certain bacteria known for their resistance against conventional
antibiotics. The results indicated that the synthesized SNPs were crystalline, spherical in nature
and their size ranged between 18.7-26.2 nm. Also, the antibacterial sensitivity testing showed a
great inhibitory effect of SNPs against the test bacterial pathogens which included Proteus
vulgaris, Escherichia coli, Klebsiella pneumonia and Bacillus sp. Our finding suggest that
biologically synthesized SNPs using aqueous leaf extract of O. bascilicum has a promising
antimicrobial activity against some pathogenic bacteria with proven resistance to conventional
antibiotics.
Keywords: Silver nanoparticles, Ocimum basilicum, Extract, Bacterial resistance.
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