Efficacy of White Honey in Attenuating Histological Changes Resulting from Multivitamin-induced Hepatotoxicity in Albino Rats
doi.org/10.26538/tjnpr/v5i1.10
Keywords:
Hepatotoxicity, Liver, Multivitamins, Treatment, White honeyAbstract
The body requires vitamins to keep it healthy and strengthen the immune system, but a serious health risk may result from its excessive intake. White honey is an important natural product used in traditional medicine as an alternative treatment for clinical conditions ranging from wound healing to removal of cytotoxicity. The present research was an attempt to evaluate the anti-toxicity effect of white honey against hepatotoxicity induced by high dose of multivitamins in albino rats. Sixty male albino rats were used in this study and were divided into four experimental groups: 10 rats served as normal control (Group 1); 10 rats were administered with white honey for one month (Group 2); 20 rats were treated with high dose of multivitamins for one month (Group 3); and 20 rats were treated with high dose of multivitamins for one month, followed by white honey for additional one month (Group 4). The results showed a significant increase in body weight of Group 3 rats (23.3 kg) compared to the control (18.7 kg), while a reduction in body weight was recorded in Group 4 (20.5 kg) in relation to Group 3. A similar observation was made for liver function test parameters. The histological analysis revealed that white honey was able to restore many pathological and harmful changes resulting from the high dose of multivitamins and emergence of a clear improvement of liver tissues. Our finding therefore indicated that white honey supplementation ameliorated the harmful effect induced by high dose of multivitamins.
References
Briefel RR and Johnson CL. Secular trends in dietary intake in the United States. Annu Rev Nutr. 2004; 24:401-431.
Radimer K, Bindewald B, Hughes J, Ervin B, Swanson C, Picciano MF. Dietary supplement use by US adults: data
from the National Health and Nutrition Examination Survey, 1999–2000. Am J Epidemiol. 2004; 160(4):339-349.
Arteh JI, Narra S, Nair S. Prevalence of vitamin D deficiency in chronic liver disease. Dig Dis Sci. 2010; 55(9):2624-2628.
Prosser DE and Jones G. Enzymes involved in the activation and inactivation of vitamin D. Trends Biochem Sci. 2004; 29(12):664-673.
Radimer KL. Methodological issues in assessing dietary supplement use in children. J Acad Nutr Dietetics. 2005; 105(5):703-708.
Butler MS and Buss AD. Natural products—the future scaffolds for novel antibiotics? Biochem Pharmacol. 2006; 71(7):919-929.
Othman NH. Does honey have the characteristics of natural cancer vaccine? J Trad Compl Med. 2012; 2(4):276-283.
Othman NH. Honey and cancer: sustainable inverse relationship particularly for developing nations: A review. Evid-Based Compl Altern Med. 2012(1):406-410.
Ahmed S and Othman NH. Honey as a potential natural anticancer agent: A review of its mechanisms. Evid-Based Compl Altern Med. 2013; 2013:829070
Scepankova H, Saraiva JA, Estevinho LM. Honey health benefits and uses in medicine. In Bee Products-Chemical and Biological Properties. Springer, Cham. 2017. 83-96 p.
Viuda-Martos M, Pérez-Alvarez JA, Fernández-López J. Royal jelly: Health benefits and uses in medicine. In Bee Products-Chemical and Biological Properties. Springer, Cham. 2017. 199-218 p.
Baltas N, Karaoglu SA, Tarakci C, Kolayli S. Effect of propolis in gastric disorders: Inhibition studies on the growth of Helicobacter pylori and production of its urease. Jenzyme inhibition and medicinal chemistry. 2016; 31(supl 2):46-50.
Macegoniuk K, Grela E, Palus J, Rudzinska-Szostak E, Grabowiecka A, Biernat M, Berlicki Ł. 1,2-Benzisoselenazol-3 (2 H)-one derivatives as a new class of bacterial urease inhibitors. J Med Chem. 2016; 59(17):8125-8133.
Denisow B and Denisow‐Pietrzyk M. Biological and therapeutic properties of bee pollen: a review. J Sci Food Agric. 2016; 96(13):4303-4309.
Hamza RZ, Diab AZ, Abd El-Aziz EA, Hendawy AA. Immunotoxic effect of organophosphorus insecticides chlorpyrifos, profenofos, and possible ameliorative role of propolis and ginseng. Biosci Biotech Res Asia. 2013; 10(2):645-651.
Al-Waili NS. Effects of daily consumption of honey solution on hematological indices and blood levels of minerals and enzymes in normal individuals. J Med Food. 2003; 6(2):135-140.
Rzepecka-Stojko A, Stojko J, Kurek-Górecka A, Górecki M, Kabała-Dzik A, Kubina R, Buszman E. Polyphenols from bee pollen: structure, absorption, metabolism and biological activity. Molecules. 2015; 20(12):21732-21749.
Nagai T, Sakai M, Inoue R, Inoue H, Suzuki N. Antioxidative activities of some commercially honeys, royal jelly, and propolis. Food Chem. 2001; 75(2):237-240.
Al Othman SI, Khalif Alanazi F, Sharahili GJ, Alzahrani AA, Shahrani RD, Alshehri WM, Suli A. The effectiveness of pectin in the reduction of histological changes caused by exposure to monosodium glutamate in female mice.Pakistan J Biol Sci. 2020; (23):197-182.
Wang Y, Li D, Cheng N, Gao H, Xue X, Cao W, Sun L. Antioxidant and hepatoprotective activity of Vitex honey against paracetamol induced liver damage in mice. Food Funct. 2015; 6(7):2339-2349.
Juszczak L, Florkiewicz A, Socha R, Gałkowska D. Effect of honey supplementation with bee products on quality parameters and mineral composition. Emirates J Food Agric. 2019; 30:990–997.
Siegel RL, Miller KD, Jemal A. 2016 Cancer statistics. Cancer J Clin. 2016; 66(1):7-30.
Yu R. Hypercalcemic crisis associated with anastrozole use in a patient with breast cancer and primary hyperparathyroidism. Endocrine. 2016; 53(3):868-869.
Syed I, Lee J, Moraes-Vieira PM, Donaldson CJ, Sontheimer A, Aryal P, Wellenstein K, Kolar MJ, Nelson AT, Siegel D, et al. Palmitic acid hydroxystearic acids activate GPR40, which is involved in their beneficial effects on glucose homeostasis. Cell Metab. 2018; 27:419–427.
Gabr M, El-Meligy M, Mohamed H, Mohamed O. The Effects of chronic aspartame administration on the kidney of albino rat. Egyptian J Anat. 2011; 34(2):85-102.
Russ M, Jauk S, Wintersteiger R, Andrä M, Brcic I, Ortner A. Investigation of antioxidative effects of a cardioprotective solution in heart tissue. Mol Cell Biochem. 2019; 461:73–80.
Predes FS, Diamante MAS, Foglio MA, Camargo CA, Aoyama H, Miranda SC, Cruz B, Cristina M, Marcondes CG, Dolder H. Hepatoprotective effect of Arctium lappa root extract on cadmium toxicity in adult wistar rats. Biol Trace Elem Res. 2014; 160:250–257.
Pavithra D, Praveen D, Chowdary PR, Aanandhi MV. A review on role of Vitamin E supplementation in type 2diabetes mellitus. Drug Invent Today. 2018; 10(2):236–240.
Miller ER, Pastor-Barriuso R, Dalal D, Riemersma RA, Appel LJ, Guallar E. Meta-analysis: high-dosage vitamin E supplementation may increase all-cause mortality. Ann Intern Med. 2005; 142(1):37–46.
Alqadhi YA, Waykar B, De S, Pal A. Biological properties and uses of honey: A concise scientific review. Indian J
Pharm Bio Res. 2016; 4(03):58-68.
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