Evaluation of Antidiabetic Potential of Extract of Sonneratia caseolaris (L.) Engl. Leaves against Alloxan-Induced Diabetes in Mice

doi.org/10.26538/tjnpr/v5i1.9

Authors

  • Apurba K. Barman Department of Pharmacy, School of Life Science and Health, Ranada Prasad Saha University, Naryanganj-1400, Bangladesh
  • Tausif Ahmed Department of Pharmacy, School of Life Science and Health, Ranada Prasad Saha University, Naryanganj-1400, Bangladesh
  • Himu Das Department of Pharmacy, School of Life Science and Health, Ranada Prasad Saha University, Naryanganj-1400, Bangladesh
  • Biswajit Biswas Department of Pharmacy, Faculty of Biological Science & Technology, Jashore University of Science and Technology, Jashore 7408, Bangladesh
  • Md. Sihab Ali Department of Aerospace medicine, CMH, Dhaka 1206, Dhaka, Bangladesh
  • Rabindra N. Acharyya Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • Kishore K. Sarkar epartment of Pharmacy, Faculty of Biological Science & Technology, Jashore University of Science and Technology, Jashore 7408, Bangladesh
  • Shrabanti Dev Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh

Keywords:

Sonneratia caseolaris, Antioxidant, Alpha amylase, Antidiabetic

Abstract

Sonneratia caseolaris (L.), a mangrove plant of the Sundarbans have traditionally been used as an antidiabetic agent. The study investigated the potential antioxidant and antidiabetic activities of the ethanol extract of the plant leaves. The antioxidant activity was evaluated using the 1,1- diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and reducing power assay. The total secondary metabolites of the plant such as the total phenolic content (TPC), total tannin content (TTC) and total flavonoid content (TFC) were also evaluated. The antidiabetic potential was evaluated using the in vitro alpha-amylase enzyme inhibitory assay and the in vivo oral glucose tolerance test (OGTT). Furthermore, Swiss albino mice were used to evaluate the effect of extract on blood glucose level of alloxan-induced diabetic mice. The extract showed the presence of a significant amount of phenolics (219.53 mg GAE/g), and flavonoids (454.88 mg QE/g). In the DPPH assay, the IC50 value of S. caseolaris was 27.0 µg/mL, while that of ascorbic acid was 12.0 µg/mL. Reducing power assay also revealed the antioxidative potential of the extract with an RC50 value of 395.5 µg/mL. The extract showed notable alpha-amylase inhibitory activity with an IC50 value of 37.6 µg/mL. In the OGTT test, extract showed a significant (p<0.05) reduction of blood glucose levels compared to control. Moreover, extract of caseolaris inhibited the diabetogenic activity of alloxan and significantly (p<0.05) controlled the blood glucose level in treated mice. The result indicated the antidiabetic potential of ethanol extract of S. caseolaris leaves, and therefore, justifies their use in folklore medicine. 

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Published

2021-01-01

How to Cite

Barman, A. K., Ahmed, T., Das, H., Biswas, B., Ali, M. S., Acharyya, R. N., … Dev, S. (2021). Evaluation of Antidiabetic Potential of Extract of Sonneratia caseolaris (L.) Engl. Leaves against Alloxan-Induced Diabetes in Mice: doi.org/10.26538/tjnpr/v5i1.9. Tropical Journal of Natural Product Research (TJNPR), 5(1), 77–83. Retrieved from https://tjnpr.org/index.php/home/article/view/821

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