Evaluation of Antidiabetic Potential of Extract of Sonneratia caseolaris (L.) Engl. Leaves against Alloxan-Induced Diabetes in Mice doi.org/10.26538/tjnpr/v5i1.9
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Abstract
Sonneratia caseolaris (L.), a mangrove plant of the Sundarbans have traditionally been used as an antidiabetic agent. The study investigated the potential antioxidant and antidiabetic activities of the ethanol extract of the plant leaves. The antioxidant activity was evaluated using the 1,1- diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and reducing power assay. The total secondary metabolites of the plant such as the total phenolic content (TPC), total tannin content (TTC) and total flavonoid content (TFC) were also evaluated. The antidiabetic potential was evaluated using the in vitro alpha-amylase enzyme inhibitory assay and the in vivo oral glucose tolerance test (OGTT). Furthermore, Swiss albino mice were used to evaluate the effect of extract on blood glucose level of alloxan-induced diabetic mice. The extract showed the presence of a significant amount of phenolics (219.53 mg GAE/g), and flavonoids (454.88 mg QE/g). In the DPPH assay, the IC50 value of S. caseolaris was 27.0 µg/mL, while that of ascorbic acid was 12.0 µg/mL. Reducing power assay also revealed the antioxidative potential of the extract with an RC50 value of 395.5 µg/mL. The extract showed notable alpha-amylase inhibitory activity with an IC50 value of 37.6 µg/mL. In the OGTT test, extract showed a significant (p<0.05) reduction of blood glucose levels compared to control. Moreover, extract of caseolaris inhibited the diabetogenic activity of alloxan and significantly (p<0.05) controlled the blood glucose level in treated mice. The result indicated the antidiabetic potential of ethanol extract of S. caseolaris leaves, and therefore, justifies their use in folklore medicine.
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Styskal J, Remmen VH, Richardson A, Salmon AB. Oxidative stress and diabetes: What can we learn about insulin resistance from antioxidant mutant mouse models? Free Radic Biol Med. 2012; 52(1):46-58.
Finkel T and Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature 2000; 408(6809):239-247.
Johansen JS, Harris AK, Rychly DJ, Ergul A. Oxidative stress and the use of antioxidants in diabetes: Linking basic science to clinical practice. Cardiovasc Diabetol. 2005; 4:5.
Debnath SL, Kundu P, Golder M, Biswas B, Sadhu SK. Phytochemical characterization and evaluation of pharmacological activities of leaves of a mangrove plant species - Aegiceras corniculatum (L.). Trop J Nat Prod Res. 2020;
(9):516-522.
Golder M, Sadhu SK, Biswas B, Islam T. Comparative pharmacologic profiles of leaves and hypocotyls of a mangrove
plant: Bruguiera gymnorrhiza. Adv Tradit Med. 20(3):395-403.
Zimmet P, Alberti KG, Magliano DJ, Bennett PH. Diabetes mellitus statistics on prevalence and mortality: Facts and fallacies. Nat Rev Endocrinol. 2016; 12:616-622.
Mayfield JA. Diagnosis and classification of diabetes mellitus: New criteria. Am Fam Physician. 1998; 58(6):1355-1362.
Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010; 4(8):118-126.
Krentz AJ and Bailey CJ. Oral antidiabetic agents. Drugs 2005; 65(3):385-411.
Chaudhury RR and Rafe UM. Traditional medicine in Asia. New Delhi, India: World Health Organization Regional Office for South-East Asia; 2002.
Xu X, Shan B, Liao CH, Xie JH, Wen PW, Shi JY. Anti-diabetic properties of Momordica charantia L. polysaccharide in alloxaninduced diabetic mice. Int J Biol Macromol. 2015; 81:538-543.
Sadhu SK, Ahmed F, Ohtsuki T, Ishibashi M. Flavonoids from Sonnertia caseolaris. J Nat Med. 2006; 60(3):264-265.
Ghani A. Medicinal plants of Bangladesh: Chemical constituents and uses. Dhaka, Bangladesh: Asiatic society of Bangladesh; 1998.
Tian M, Dai H, Li X, Wang B. Chemical constituents of marine medicinal mangrove plant Sonneratia caseolaris. Chin J Oceanol Limnol. 2009; 27(2):288-296.
Wetwitayaklung P, Limmatvapirat C, Phaechamud T. Antioxidant and anticholinesterase activities in various parts of
Sonneratia caseolaris (L.). Indian J Pharm Sci. 2013; 75(6):649-656.
Bandaranayake W. Traditional and medicinal uses of mangroves. Mang Salt Marshes. 1998; 2:133-148.
Devi P, Solimabi W, D’souza L, Kamat SY. Toxic effects of coastal and marine plant extracts on mosquito larvae. Bot Mar. 1997; 40(1-6):533-536.
Tiwari AK, Viswanadh V, Gowri PM, Ali AZ, Radhakrishnan SVS, Agawane SB, Madhusudana K, Rao JM. Oleanolic acid -
an α-glucosidase inhibitory and antihyperglycemic active compound from the fruits of Sonneratia caseolaris. Open Access J Med Aromat Plants. 2010; 1(1):19-23.
Egharevba E, Chukwuemeke-Nwani P, Eboh U, Okoye E, Bolanle IO, Oseghale IO, Imieje VO, Erharuyi O, Falodun A.
Evaluation of the antioxidant and hypoglycaemic potentials of the leaf extracts of Stachytarphyta jamaicensis Verbenaceae). Trop J Nat Prod Res. 2019; 3(5):170-174.
Biswas B, Golder M, Islam T, Sadhu SK. Comparative antioxidative and antihyperglycemic profiles of neumatophores
of two mangrove species Avicennia alba and Sonneratia apetala. Dhaka Univ J Pharm Sci. 2018; 17(2):205-211.
Kumar S, Sandhir R, Ojha S. Evaluation of antioxidant activityand total phenol in different varieties of Lantana camara leaves. BMC Res Notes. 2014; 7:560.
Xiao Z, Storms R, Tsang A. A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities. Anal Biochem. 2006; 351(1):146-148.
Saleem U, Amin S, Ahmad B, Azeem H, Anwar F, Mary S. Acute oral toxicity evaluation of aqueous ethanolic extract of
Saccharum munja Roxb. roots in albino mice as per OECD 425 TG. Toxicol Rep. 2017; 4:580-585.
Ahmed MF, Kazim SM, Ghori SS, Mahjabeen SS, Ahmed SR, Ali SM, Ibrahim M. Antidiabetic activity of Vinca rosea extracts in alloxan-induced diabetic rats. Int J Endocrinol. 2010; 2010.
Sarkar KK, Rahman MM, Shahriar AAE, Mitra T, Golder M, Zilani MNH, Biswas B. Comparative neuropharmacological and cytotoxic profiles of Alstonia scholaris (L.) and Mimusops elengi(L.) leaves. Adv Trad Med. 2020. https://doi.org/10.1007/s13596-020-00463-5.
Rahmatullah M, Azam MNK, Pramanik S, Sania, Rahman S, Jahan R. Antihyperglycemic activity evaluation of rhizomes of Curcuma zedoaria (Christm.) Roscoe and fruits of Sonneratia caseolaris (L.) Engl. Int J Pharmtech Res. 2012; 4(1):125-129.
Razali N, Razab R, Junit SM, Aziz AA. Radical scavenging and reducing properties of extracts of cashew shoots (Anacardium occidentale). Food Chem. 2008; 111(1):38-44.
Talukder C, Saha S, Adhikari S, Mondal HK, Islam MK, Anisuzzman M. Evaluation of antioxidant, analgesic and
antidiarrhoeal activity of Flacourtia jangomas (Lour.) Raeusch. Leaves. Pharmacologyonline. 2012; 3:20-28.
Jayanthi P and Lalitha P. Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. Int J Pharm Pharm Sci. 2011; 3(3):126-128.
Dai J and Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15(10):7313-7352.
Haslam E. Natural polyphenols (vegetable tannins) as drugs: Possible modes of action. J Nat Prod. 1996; 59(2):205-215.
Gutteridge JM. Biological origin of free radicals, and mechanisms of antioxidant protection. Chem Biol Interact. 1994;
(2-3):133-140.
Kumar G, Arulselvan P, Kumar D, Subramanian S. Anti-diabetic activity of fruits of Terminalia chebula on streptozotocin induced diabetic rats. J Health Sci. 2006; 52(3):283-291.
Cheng AY and Fantus IG. Oral antihyperglycemic therapy for type 2 diabetes mellitus. CMAJ. 2005; 172(2):213-226.
Tiwari A. Wisdom of Ayurveda in perceiving diabetes: Enigma of therapeutic recognition. Curr Sci. 2005; 88(7):1043-1051.
Tadera K, Minami Y, Takamatsu K, Matsuoka T. Inhibition of alpha-glucosidase and alpha-amylase by flavonoids. J Nutr Sci Vitaminol. 2006; 52(2):149-153.
McDougall GJ, Kulkarni NN, Stewart D. Current developments on the inhibitory effects of berry polyphenols on digestive enzymes. Biofactors 2008; 34(1):73-80.
American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2009; 32(1):62-67.
Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001;
(6):537-546.
Olmedilla B, Granado F, Gil-Martinez E, Blanco I, RojasHidalgo E. Reference values for retinol, tocopherol, and main
carotenoids in serum of control and insulin-dependent diabetic Spanish subjects. Clin Chem. 1997; 43(6):1066-1071.
Kumari S, Kumar P, Wanjari M, Palani S. Antidiabetic activity of Pandanus fascicularis Lamk - aerial roots in alloxan-induced hyperglycemic rats. Int J Nutr Pharmacol Neurol Dis. 2012; 2(2):105-110.
Chakravarthy Bk, Gupta S, Gambhir SS, Gode KD. Pancreatic beta cell regeneration - a novel antidiabetic mechanism of Pterocarpus marsupium roxb. Indian J Pharmacol. 1980; 12(2):123-127.
Choi R, Kim BH, Naowaboot J, Lee MY, Hyun MR, Cho EJ, Lee ES, Lee EY, Yang YC, Chung CH. Effects of ferulic acid on
diabetic nephropathy in a rat model of type 2 diabetes. Exp Mol Med. 2011; 43(12):676-683.