Thunbergia laurifolia Linn. Extract Protects Ethanol Addiction and Increases Dopamine Synthesis doi.org/10.26538/tjnpr/v5i1.5
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Abstract
Thunbergia laurifolia Linn. is a plant used in traditional medicine for the detoxification of several substances including ethanol. Previous studies showed that T. laurifolia increased neuronal activity and altered the dopaminergic system in various brain areas similar to the effect of amphetamine and cocaine, but did not produce addiction in a chronic study. In the present study, we investigated the protective effect of T. laurifolia on behavioural changes and alteration of the tyrosine hydroxylase expressions in the mesolimbic dopaminergic pathway in acute and chronic ethanol administration in adult male Wistar rats. The animals were divided into four groups i.e., control, ethanol, T. laurifolia (200 mg/kg, i.p.), and T. laurifolia (200 mg/kg, i.p.) with ethanol in both acute and chronic (30 days) administrations. The anxiety behaviour, exploratory behaviour, and addiction behaviour were tested and tyrosine hydroxylase expression alteration was determined. Chronic ethanol administration significantly (p < 0.01) induced addictive behaviour in the conditioned place preference (CPP) test and significantly (p < 0.01) decreased tyrosine hydroxylase expression in the nucleus accumbens. Chronic T. laurifolia administration alone did not cause any change in the behaviours and tyrosine hydroxylase expression. Interestingly, daily administration of T. laurifolia prior to ethanol administration prevented ethanol-induced addictive behaviour and significantly (p 0.01) increased tyrosine hydroxylase expression in the nucleus accumbens. Therefore, it can be concluded from our results that daily pre-administration of T. laurifolia can protect against ethanol-induced addiction and a decrease in dopamine synthesis in the reward system of the mesolimbic dopaminergic pathway.
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Sel E and Dinç K. [Neurobiology of alcohol dependence and implications on treatment]. Turk Psikiyatri Derg. 2017; 28(1):51-60.
Klenowski PM and Tapper AR. Molecular, neuronal, and behavioral effects of ethanol and nicotine interactions. Handb Exp Pharmacol. 2018; 248:187-212.
You C, Vandegrift B, Brodie MS. Ethanol actions on the ventral tegmental area: novel potential targets on reward pathway neurons. Psychopharmacol. 2018;235(6):1711-1726.
Aurelian L, Warnock KT, Balan I, Puche A, June H. TLR4 signaling in VTA dopaminergic neurons regulates impulsivity through tyrosine hydroxylase modulation. Transl Psychiatry. 2016; 6(5):e8-e15.
Deehan GA Jr, Knight CP, Waeiss RA, Engleman EA, Toalston JE, McBride WJ, Hauser SR, Rodd ZA. Peripheral administration of ethanol results in a correlated increase in dopamine and serotonin within the posterior ventral tegmental area. Alcohol 2016;51(5):535-540.
Abrahao KP, Salinas AG, Lovinger DM. Alcohol and the brain: neuronal molecular targets, synapses, and circuits. Neuron. 2017; 96(6):1223-1238.
Karkhanis AN, Huggins KN, Rose JH, Jones SR. Switch from excitatory to inhibitory actions of ethanol on dopamine levels after chronic exposure: role of kappa opioid receptors. Neuropharmacol. 2016;110(A):190-197.
Morel C, Montgomery S, Han M-H. Nicotine and alcohol: the role of midbrain dopaminergic neurons in
drug reinforcement. Eur J Neurosci. 2019; 50(3):2180-2200.
Small KM, Nunes E, Hughley S, Addy NA. Ventral tegmental area muscarinic receptors modulate depression and anxiety-related behaviors in rats. Neurosci Lett. 2016; 616:80-85.
Zeng K, Xie A, Zhang X, Zhong B, Liu X, Hao W. Chronic alcohol treatment-induced GABA-Aα5 histone H3K4 trimethylation upregulation leads to increased GABA-Aα5 expression and susceptibility to alcohol addiction in the offspring of Wistar rats. Front Psychiatry. 2018; 9:468-474.
Pati D, Pina MM, Kash TL. Ethanol-induced conditioned place preference and aversion differentially alter plasticity in the bed nucleus of stria terminalis. Neuropsychopharmacol. 2019;44(11):1843-1854.
Vengeliene V, Bilbao A, Molander A, Spanagel R. Neuropharmacology of alcohol addiction. Br J Pharmacol. 2008; 154(2):299-315.
Sena MC, Nunes FC, Salvadori MG, Carvalho CC, Morais LC, Braga VA. Chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice. Clinics (Sao Paulo). 2011; 66(5):873-878.
Hansen AW, Almeida FB, Bandiera S, Pulcinelli RR, Fragoso ALR, Schneider R Jr, Barros HMT, Gomez R. Taurine restores the exploratory behavior following alcohol withdrawal and decreases BDNF mRNA expression in the frontal cortex of chronic alcohol-treated rats. Pharmacol Biochem Behav. 2017; 161:6-12.
Ussanawarong S and Thesiri T. Effect of Thunbergia laurifolia Linn. on detoxication of parathion in rat. KKU Res J. 2001; 6:3-13.
Wonkchalee O, Boonmars T, Aromdee C, Laummaunwai P, Khunkitti W, Vaeteewoottacharn K, Sriraj P, Aukkanimart R, Loilome W, Chamgramol Y, Pairojkul C, Wu Z, Juasook A, Sudsarn P. Antiinflammatory, antioxidant and epatoprotective effects of Thunbergia laurifolia Linn. on experimental opisthorchiasis. Parasitol Res. 2012; 111(1):353-359.
Nanna U, Chiruntanat N, Jaijoy K, Rojsanga P, Sireeratawong S. Effect of Thunbergia laurifolia Lindl. extract on anti-inflammatory, analgesic and antipyretic activity. J Med Assoc Thai. 2017;100(Suppl. 5):S98-S106.
Oonsivilai R, Cheng C, Bomser J, Ferruzzi MG, Ningsanond S. Phytochemical profiling and phase II enzyme-inducing properties of Thunbergia laurifoliaLindl. (RC) extracts. J Ethnopharmacol. 2007;114(3):300-306.
Rojsanga P, Raksaskulwong G, Ruaysaptawee K,Chooluck K. Preliminary findings of the effect of infusion variables on marker contents and antioxidant activity of Thunbergia laurifolia tea. Pharm Sci Asia. 2018; 45:243-251.
Kanchanapoom T, Kasai R, Yamasaki K. Iridoid glucosides from Thunbergia laurifolia. Phytochem.2002; 60(8):769-771.
Thongsaard W, Marsden CA, Morris P, Prior M, Shah YB. Effect of Thunbergia laurifolia, a Thai natural product used to treat drug addiction, on cerebral activity detected by functional magnetic resonance imaging in the rat. Psychopharmacol (Berl). 2005; 180(4):752-760.
Thongsaard W and Marsden C. Effect of Thunbergia laurifolia extract on extracellular dopamine level in rat nucleus accumbens. J Med Assoc Thai. 2013;96(Suppl. 1):S85-S89.
Huang Z and Sjöholm A. Ethanol acutely stimulates islet blood flow, amplifies insulin secretion, and induces hypoglycemia via nitric oxide and vagally mediated mechanisms. Endocrinology 2008;149(1):232-236.
Deachapunya C and Thongsaard W. Behavioral effects of acute and chronic oral administration of barakol in rats. J Med Assoc Thai. 2009; 92 (Suppl. 3):S29-S37.
Thongsaard W, Sangpayap R, Marsden C. Chronic effect of Thunbergia laurifolia extract and cocaine in rats using behavior model of addiction. J Med Assoc Thai. 2015; 98(Suppl 9):S48-S52.
Leeboonngam T, Pramong R, Sae-Ung K, Govitrapong P, Phansuwan-Pujito P. Neuroprotective effects of melatonin on amphetamine-induced dopaminergic fiber degeneration in the hippocampus of postnatal rats. J Pineal Res. 2018; 64(3):e12456.
Paxinos G and Watson C. The rat brain in stereotaxic coordinates. (5th ed.). New York: Academic Press; 2005.94-110p.
Kaewsuk S, Sae-ung K, Phansuwan-Pujito P, Govitrapong P. Melatonin attenuates methamphetamine-induced reduction of tyrosine hydroxylase, synaptophysin and growth-associated protein-43 levels in the neonatal rat brain. Neurochem Int. 2009; 55(6):397-405.
Siciliano CA, Calipari ES, Yorgason JT, Lovinger DM, Mateo Y, Jimenez VA, Helms CM, Grant KA, Jones SR. Increased presynaptic regulation of dopamine neurotransmission in the nucleus accumbens core following chronic ethanol self-administration in female macaques. Psychopharmacol (Berl). 2016;233(8):1435-1443.
Lovinger DM and Roberto M. Synaptic effects induced by alcohol. Curr Top Behav Neurosci. 2013;13:31-86.
Melchior JR and Jones SR. Chronic ethanol exposure increases inhibition of optically targeted phasic dopamine release in the nucleus accumbens core and medial shell ex vivo. Mol Cell Neurosci. 2017; 85:93-104.
Trantham-Davidson H and Chandler LJ. Alcoholinduced alterations in dopamine modulation of prefrontal activity. Alcohol 2015; 49(8):773-779.
Wille-Bille A, de Olmos S, Marengo L, Chiner F, Pautassi RM. Long-term ethanol self-administration induces ΔFosB in male and female adolescent, but not in adult, Wistar rats. Prog Neuropsychopharmacol Biol Psychiatry. 2017; 74:15-30.
Rocejanasaroj A, Tencomnao T, Sangkitikomol W. Thunbergia laurifolia extract minimizes the adverse effects of toxicants by regulating P-glycoprotein activity, CYP450, and lipid metabolism gene expression in HepG2 cells. Genet Mol Res. 2014;13(1):205-219.
Atilano-Roque A, Roda G, Fogueri U, Kiser JJ, Joy MS. Effect of disease pathologies on transporter expression and function. J Clin Pharmacol. 2016;56(Suppl. 7):S205-S221.
Auvity S, Caillé F, Marie S, Wimberley C, Bauer M, Langer O, Buvat I, Goutal S, Tournier N. Pglycoprotein (ABCB1) inhibits the influx and increases the efflux of (11)c-metoclopramide across the blood-brain barrier: a PET study on nonhuman primates. J Nucl Med. 2018; 59(10):1609-1615.
Efferth T and Volm M. Multiple resistance to carcinogens and xenobiotics: P-glycoproteins as universal detoxifiers. Arch Toxicol. 2017; 91(7):2515-2538.
García-Suástegui WA, Ramos-Chávez LA, RubioOsornio M, Calvillo-Velasco M, Atzin-Méndez JA, Guevara J, Silva-Adaya D. The role of CYP2E1 in the drug metabolism or bioactivation in the brain. Oxid Med Cell Longev. 2017; 2017:4680732.
Peana AT, Sánchez-Catalán MJ, Hipólito L, Rosas M, Porru S, Bennardini F, Romualdi P, Caputi FF, Candeletti S, Polache A, Granero L, Acquas E. Mystic acetaldehyde: the never-ending story on alcoholism. Front Behav Neurosci. 2017; 11:81.
Brancato A, Lavanco G, Cavallaro A, Plescia F, Cannizzaro C. Acetaldehyde, motivation and stress: behavioral evidence of an addictive ménage à trois. Front Behav Neurosci. 2017; 11:23.
Tangpong J and Satarug S. Alleviation of lead poisoning in the brain with aqueous leaf extract of the Thunbergia laurifolia (Linn.). Toxicol Lett. 2010;198(1):83-88.
Pramyothin P, Chirdchupunsare H, Rungsipipat A, Chaichantipyuth C. Hepatoprotective activity of Thunbergia laurifolia Linn extract in rats treated with ethanol: in vitro and in vivo studies. J Ethnopharmacol. 2005; 102(3):408-411.
Ruangyuttikarn W, Chattaviriya P, Morkmek N, Chuncharunee S, Lertprasertsuke N. Thunbergia laurifolia leaf extract mitigates cadmium toxicity in rats. Sci Asia. 2013; 39(1):19-25.
Chivapat S, Chavalittumrong P, Attawish A, Bansiddhi J, Padungpat S. Chronic toxicity of Thunbergia laurifolia Lindl. extract. J Thai Trad Altern Med. 2010; 7:17-25.
Karlsson O and Roman E. Dose-dependent effects of alcohol administration on behavioral profiles in the MCSF test. Alcohol 2016; 50:51-56.
Peltier MR, Verplaetse TL, Mineur YS, Petrakis IL, Cosgrove KP, Picciotto MR, McKee SA. Sex differences in stress-related alcohol use. Neurobiol Stress. 2019; 10:100149.
Lister RG. The effects of ethanol on exploration in DBA/2 and C57Bl/6 mice. Alcohol 1987; 4(1):17-19.
Zhang ZB, Xu TY, You DY, Yi S, Liu Q, Li HJ, Gu JY. The interactive effects of ketamine and ethanol on dopamine expression in the ventral tegmental area of rats. Neuropsychiatr Dis Treat. 2018; 14:2105-2114.
Liu Y, Montgomery SE, Juarez B, Morel C, Zhang S, Kong Y, Calipari ES, Nestler EJ, Zhang L, Han MH. Different adaptations of dopamine release in nucleus accumbens shell and core of individual alcohol drinking groups of mice. Neuropharmacol. 2020;175:108176.
Oliva JM, Ortiz S, Perez-Rial S, Manzanares J. Time dependent alterations on tyrosine hydroxylase, opioid and cannabinoid CB1 receptor gene expressions after acute ethanol administration in the rat brain. Eur Neuropsychopharmacol. 2008; 18(5):373-382.
Bouarab C, Thompson B, Polter AM. VTA GABA neurons at the interface of stress and reward. Front Neural Circuits. 2019; 13:78.