Lycopene for Wound Infection: In-Vitro Susceptibility of Drug-Resistant Clinical Pathogens


  • Chinyere C. Ezeanya-Bakpa Department of Medical Microbiology, Edo University Iyamho, Nigeria
  • Charles O. Adetunji Department of Microbiology, Applied Microbiology, Biotechnology and Nanotechnology Laboratory, Edo University Iyamho, Nigeria
  • Israel A. Enosabata Department of Microbiology, Edo University Iyamho, Nigeria
  • Eric Olori Department of Chemistry, Edo University Iyamho, Nigeria


Wound infections caused by drug-resistant pathogens have limited treatment options,

consequently the need to screen new antimicrobials. Phytochemicals have been adopted as

sources for new antimicrobials. Consequently, the study aimed to estimate the antibacterial

activity of lycopene phytochemical against drug-resistant wound pathogens: Staphylococcus

aureus, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Klebsiella

pneumoniae. The antibiotic susceptibility profiles of all the resistant clinical isolates to

commonly administered antibiotics were determined using disc diffusion method. The drug

resistant Gram-negative and Gram-positive bacteria (both groups) isolated from infected wounds

were investigated for their in-vitro susceptibility to the lycopene extracted from tomato

(Lycopersicon esculentum) by agar-well diffusion assay and broth dilution method. Differences

of mean zone of inhibitions (ZOIs), minimum inhibitory concentrations (MICs) and minimum

bactericidal concentrations (MBCs) values among the drug-resistant wound isolates were

considered significant where P ˂ 0.05. All isolates were resistant to Augmentin, Gentamicin,

Ciprofloxacin and Streptomycin. In-vitro susceptibility to lycopene revealed the mean and

standard deviation of the ZOIs for all isolates as 7.6 ± 3.49 mm. The studied lycopene showed

bactericidal effects against all the drug-resistant bacteria tested. The MICs and MBCs of the

wound isolates ranged from 50 and 125 μg/mL. The difference between the mean ZOIs,

MICs/MBCs of both groups were not significant. This study demonstrated that lycopene has

antibacterial activity against some drug-resistant wound isolates thus, offers a good alternative to

existing treatment options for wound infections.

Keywords: Wound infection, Lycopene, Antibacterial activity, Drug-resistant pathogens, Agar

well diffusion assay.


Kassam NA, Damian DJ, Kajeguka D, Nyombi, B, Kibik GS. Spectrum and antibiogram of bacteria isolated from patients

presenting with infected wounds in a Tertiary Hospital, northern Tanzania. BMC Res Notes. 2017; 10:757.

Silva V, Marcoleta A, Silva V, Flores D, Aparicio T, Aburto I, Latrach C, Febré N. Prevalence and susceptibility pattern of bacteria isolated from infected chronic wounds in adult patients. Revista Chilena de Infectologia. 2018; 35(2):155-162.

Adeyemi K, Pelumi O, Abiodun O, Oyeronke A, Abdul-Wasiu H, Atilola G. High prevalence of multiple drug-resistant enteric bacteria: Evidence from a teaching hospital in South west Nigeria. J Infect Pub Health. 2020; 13(4):651-656.

Akujobi CN, Ezeanya CC, Aghanya NI. Detection of Cefotaximase Genes of Beta Lactamase among Clinical Isolates of Escherichia coli in a University Teaching Hospital, Nigeria. J Med Sci. 2012; 12:244-247.

Sisay M, Worku T, Edessa D. Microbial epidemiology and antimicrobial resistance patterns of wound infection in Ethiopia: a meta-analysis of laboratory-based cross-sectional studies. BMC Pharmacol Toxicol. 2019; 20:35.

Barbieri R, Coppo E, Marchese A, Daglia M, Sobarzo-Sánchez E, Nabavi SF, Nabavi SM. Phytochemicals for human disease: An update on plant-derived compounds antibacterial activity.Microbiol Res. 2017; 196:44-68.

Maitra J and Sangeeta A. Antibacterial property of lycopene extracted from Lycopersicon esculentum against P. aeruginosa. European J Biomed Pharm Sci. 2016; 3(9):353-356.

Kushwaha K, Saini A, Saraswat P, Agarwal MK, Saxena I. Colourful world of microbes: Carotenoids and their applications. Adv. Biol 2014; article ID 837891: 13 pages

Simões M, Lemos M, Simões LC. Phytochemicals against Drug-Resistant Microbes. Dietary Phytochemicals and Microbes. Springer, Dordrecht, 2012. 185-205 p.

CLSI. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved Standard, 25th edition; Informational Supplement M100-S25. Wayne, PA, USA, 2015. 35 p.

Simran L and Vrinda N. Extraction and isolation of lycopene from various natural sources. J Biotech Biochem. 2015; 1(5):49-51.

Aghel N, Ramezani Z, Amirfakharian S. Isolation and quantification of lycopene from tomato cultivation in Dezfoul,

Iran. Jundishapur J Nat Pharm Prod. 2011; 6(1):9-15.

Kavitha G, Kanimozhi K, Panneerselvam A. Antimicrobial efficacy of lycopene compound against some pathogens. Int J Cur Res. 2017; 9(5):50184-50186.

Natividad LR and Rafael R. Carotenoid analyses and antibacterial assay of annato (Bixa orellana L.), carrot (Daucus

carota L.), corn (Zea mays L.) and tomato (Solanum lycopersicum L.) extracts. Res J Recent Sci. 2014; 3(3):40-45.

Unnisa N, Tabassum H, Naiman AM, Ponia K. Evaluation of antibacterial activity of five selected fruits on bacterial wound isolates. Int J Pharm Bio Sci. 2012; 3(4):531-546.

Azadeh RN, Elham R, Ranjbar A, Ranjbar EN. Antimicrobial property of Lycopene Oleoresin on some food pathogens. Iranian Food Sci Tech Res J. 2016; 12(3):382-387.

Khameneh B, Iranshahy M, Soheili V, Bazzaz BSF. Review on plant antimicrobials: a mechanistic viewpoint. Antimicrob Resist Infect Contr. 2019; 8:118.




How to Cite

Ezeanya-Bakpa, C. C., Adetunji, C. O., Enosabata, I. A., & Olori, E. (2021). Lycopene for Wound Infection: In-Vitro Susceptibility of Drug-Resistant Clinical Pathogens: Tropical Journal of Natural Product Research (TJNPR), 5(1), 49–52. Retrieved from