Lycopene for Wound Infection: In-Vitro Susceptibility of Drug-Resistant Clinical Pathogens doi.org/10.26538/tjnpr/v5i1.4

Main Article Content

Chinyere C. Ezeanya-Bakpa
Charles O. Adetunji
Israel A. Enosabata
Eric Olori

Abstract

Wound infections caused by drug-resistant pathogens have limited treatment options,


consequently the need to screen new antimicrobials. Phytochemicals have been adopted as


sources for new antimicrobials. Consequently, the study aimed to estimate the antibacterial


activity of lycopene phytochemical against drug-resistant wound pathogens: Staphylococcus


aureus, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Klebsiella


pneumoniae. The antibiotic susceptibility profiles of all the resistant clinical isolates to


commonly administered antibiotics were determined using disc diffusion method. The drug


resistant Gram-negative and Gram-positive bacteria (both groups) isolated from infected wounds


were investigated for their in-vitro susceptibility to the lycopene extracted from tomato


(Lycopersicon esculentum) by agar-well diffusion assay and broth dilution method. Differences


of mean zone of inhibitions (ZOIs), minimum inhibitory concentrations (MICs) and minimum


bactericidal concentrations (MBCs) values among the drug-resistant wound isolates were


considered significant where P ˂ 0.05. All isolates were resistant to Augmentin, Gentamicin,


Ciprofloxacin and Streptomycin. In-vitro susceptibility to lycopene revealed the mean and


standard deviation of the ZOIs for all isolates as 7.6 ± 3.49 mm. The studied lycopene showed


bactericidal effects against all the drug-resistant bacteria tested. The MICs and MBCs of the


wound isolates ranged from 50 and 125 μg/mL. The difference between the mean ZOIs,


MICs/MBCs of both groups were not significant. This study demonstrated that lycopene has


antibacterial activity against some drug-resistant wound isolates thus, offers a good alternative to


existing treatment options for wound infections.


Keywords: Wound infection, Lycopene, Antibacterial activity, Drug-resistant pathogens, Agar


well diffusion assay.

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How to Cite
Ezeanya-Bakpa, C. C., Adetunji, C. O., Enosabata, I. A., & Olori, E. (2021). Lycopene for Wound Infection: In-Vitro Susceptibility of Drug-Resistant Clinical Pathogens: doi.org/10.26538/tjnpr/v5i1.4. Tropical Journal of Natural Product Research (TJNPR), 5(1), 49-52. https://tjnpr.org/index.php/home/article/view/815
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How to Cite

Ezeanya-Bakpa, C. C., Adetunji, C. O., Enosabata, I. A., & Olori, E. (2021). Lycopene for Wound Infection: In-Vitro Susceptibility of Drug-Resistant Clinical Pathogens: doi.org/10.26538/tjnpr/v5i1.4. Tropical Journal of Natural Product Research (TJNPR), 5(1), 49-52. https://tjnpr.org/index.php/home/article/view/815

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