Lycopene for Wound Infection: In-Vitro Susceptibility of Drug-Resistant Clinical Pathogens
doi.org/10.26538/tjnpr/v5i1.4
Abstract
Wound infections caused by drug-resistant pathogens have limited treatment options,
consequently the need to screen new antimicrobials. Phytochemicals have been adopted as
sources for new antimicrobials. Consequently, the study aimed to estimate the antibacterial
activity of lycopene phytochemical against drug-resistant wound pathogens: Staphylococcus
aureus, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Klebsiella
pneumoniae. The antibiotic susceptibility profiles of all the resistant clinical isolates to
commonly administered antibiotics were determined using disc diffusion method. The drug
resistant Gram-negative and Gram-positive bacteria (both groups) isolated from infected wounds
were investigated for their in-vitro susceptibility to the lycopene extracted from tomato
(Lycopersicon esculentum) by agar-well diffusion assay and broth dilution method. Differences
of mean zone of inhibitions (ZOIs), minimum inhibitory concentrations (MICs) and minimum
bactericidal concentrations (MBCs) values among the drug-resistant wound isolates were
considered significant where P ˂ 0.05. All isolates were resistant to Augmentin, Gentamicin,
Ciprofloxacin and Streptomycin. In-vitro susceptibility to lycopene revealed the mean and
standard deviation of the ZOIs for all isolates as 7.6 ± 3.49 mm. The studied lycopene showed
bactericidal effects against all the drug-resistant bacteria tested. The MICs and MBCs of the
wound isolates ranged from 50 and 125 μg/mL. The difference between the mean ZOIs,
MICs/MBCs of both groups were not significant. This study demonstrated that lycopene has
antibacterial activity against some drug-resistant wound isolates thus, offers a good alternative to
existing treatment options for wound infections.
Keywords: Wound infection, Lycopene, Antibacterial activity, Drug-resistant pathogens, Agar
well diffusion assay.
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