GC-MS and Heavy Metal Analysis of Gasca D Herbal Formulation
doi.org/10.26538/tjnpr/v2i8.5
Keywords:
GC-MS,, Heavy Metals,, Toxicity,, antidiabetic.Abstract
Gasca D herbal formulation is an antidiabetic medicine produced exclusively from natural ingredients. The aim of this study was to evaluate the levels of essential and toxic heavy metals as well as to identify bioactive compounds present in Gasca D herbal formulation. The level of some essential elements (Copper, chromium, Cobalt, Iron, Manganese, Nickel and zinc) and toxic
(Cadmium, Arsenic, Vanadium, Mercury, and Lead) heavy metals were determined using microwave plasma-atomic emission spectrometry (MP-AES). The result shows no traces of Lead, Mercury, Arsenic and Zinc. Iron was found to have highest concentration 67.16 + 7.5 µg/g and Cadmium lowest concentration 0.4 + 0.03µg/g. Vanadium, the derivatives of which have been reported to have insulin-mimetic and antidiabetic properties was found at a concentration of 2.6 μg/g. The Gas Chromatography-Mass Spectrometric (GC-MS) analysis of Gasca D herbal formulation revealed the presence of 16 compounds which include N-Formyl-beta-alanine,
Paromomycin, 3,4-Altrosan, Benzamide, 1,3,4-Thiadiazol-2-amine, Carbamodithioic acid, Carbonic acid, alpha-D-Glucopyranoside, Ethyl isocyanide, 2-Propanesulfinic acid, Propanamide, 2-Butenenitrile, Dicyclopropyl carbinol, Isoxazolidine, 1,5-Hexadiene, 10-Azido-1-decanethiol.
The result indicates that the mean levels of toxic metals in Gasca D herbal formulation were below WHO permissible levels. Gasca D herbal formulation contains various bioactive compounds which may serve various medicinal applications.
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