Co-administration Effects of Newbouldia laevis (P. Beauv.) Leaves and Garcinia kola (Heckel) Seed Extracts on Animal Models of Hepatotoxicity and Diabetes
doi.org/10.26538/tjnpr/v5i2.29
Keywords:
Diabetes, Hepatotoxicity, Drug interaction, Newbouldia laevis, Garcinia kolaAbstract
Newbouldia laevis (P. Beauv.) leaves and Garcinia kola (Heckel) seeds have been demonstrated to have therapeutic applications in the treatment of diabetes and hepatic disorders. The study investigated the effects of co-administration of both plants in the treatment of diabetes and hepatopathy. The effects of the methanol extracts on alloxan-induced diabetes were determined singly and in combination at 200 and 400 mg/kg for 10 days. Similarly, the extracts and their combinations at the same doses were tested on CCl4 induced liver damage. On day 5, co-administration at 200 and 400 mg/kg produced a significant (P < 0.05) reduction in blood glucose compared with the post-induction values. Synergistic interaction between the co-administration at both doses was recorded on day 5 with a combination index of 0.63 at 400mg/kg and 0.64 at 200 mg/kg. The co-administration produced near additive effect in the reduction of diabetes-induced elevation of ALT enzyme concentration with combination index of 1.43 at 400 mg/kg and 1.29 at 200 mg/kg. For the CCl4 induced liver oxidative damage, combination of both extracts produced a synergistic reduction in serum ALT concentration at both 200 and 400 mg/kg (Combination index of 0.65 and 0.89 respectively). At lower dose (200mg/kg) combination therapy, there was synergistic reduction of serum AST (combination index of 0.83) and additive reduction at 400 mg/kg (combination index of 1.14). The combination of both extracts produced positive interaction both in the reduction of blood glucose and protection against oxidative liver damage with higher synergism recorded at lower concentration.
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