In Silico Docking Studies of Bioactive Compounds in Ocimum gratissimum Essential Oil againstCandidapepsin-1 Enzyme from Candida albicans doi.org/10.26538/tjnpr/v5i2.26
Main Article Content
Abstract
One of the most important issues of current medicine is bacterial drug resistance as well as
uropathogenic tract infections and the primary factor of these issues are extraintestinal
pathogenic E. coli strains. This study examined and compared the antibacterial activity of unripe
pawpaw peel aqueous extract (UPPA), biosynthesized silver nanoparticles (b-AgNPs) and
Ampicillin (Positive control) against E.coli. AgNPs was prepared by reducing 1mM aqueous
AgNO3 solution with UPPA. Primary characterization of synthesized AgNPs was carried out
using UV-visible spectroscopy. The total phenol content (TPC) and antibacterial activities of the
samples (UPPA and AgNPs) were carried out following standard analytical methods. Synthesis
of AgNPs was confirmed by a color change of the reaction mixture to brown after 24hrs of
incubation at room temperature, UV-visible spectroscopy also revealed an absorption maxima at
450 nm which is characteristic of AgNPs. The result of this study shows that the biosynthesized
AgNPs contains a higher amount of total phenolics and consequently showed a greater
antibacterial activity than the unripe pawpaw peel extract alone based on the zone of inhibition
exhibited by both samples on the test organism used. However , no zone of inhibition was
exhibited by the standard drug (Ampicillin) against the test organism. This study therefore
indicates that the antibacterial performance of aqueous extract of unripe pawpaw peels in
combating drug resistant pathogenic organisms could be improved to a great extent if
incorporated for the synthesis of AgNPs.
Keywords: Silver nanoparticles, Unripe pawpaw peels, Total Phenol content, Uropathogenic E.
coli.
Article Details
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