Comparative Antibacterial Activity of Biosynthesized Silver Nanoparticles and Aqueous Extract of Unripe Pawpaw Peels Against E.coli doi.org/10.26538/tjnpr/v5i2.25
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Abstract
One of the most important issues of current medicine is bacterial drug resistance as well as uropathogenic tract infections and the primary factor of these issues are extraintestinal pathogenic E. coli strains. This study examined and compared the antibacterial activity of unripe pawpaw peel aqueous extract (UPPA), biosynthesized silver nanoparticles (b-AgNPs) and Ampicillin (Positive control) against E.coli. AgNPs was prepared by reducing 1mM aqueous AgNO3 solution with UPPA. Primary characterization of synthesized AgNPs was carried out using UV-visible spectroscopy. The total phenol content (TPC) and antibacterial activities of the samples (UPPA and AgNPs) were carried out following standard analytical methods. Synthesis of AgNPs was confirmed by a color change of the reaction mixture to brown after 24hrs of incubation at room temperature, UV-visible spectroscopy also revealed an absorption maxima at 450 nm which is characteristic of AgNPs. The result of this study shows that the biosynthesized AgNPs contains a higher amount of total phenolics and consequently showed a greater antibacterial activity than the unripe pawpaw peel extract alone based on the zone of inhibition exhibited by both samples on the test organism used. However , no zone of inhibition was exhibited by the standard drug (Ampicillin) against the test organism. This study therefore indicates that the antibacterial performance of aqueous extract of unripe pawpaw peels in combating drug resistant pathogenic organisms could be improved to a great extent if incorporated for the synthesis of AgNPs.
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Ruta P, Frederic, S, Rita P, Vidmantas P, Thomas JW, Ruta P, Rasa S, Rasa B, Lilija K, Povilas K. Molecular characterization of uropathogenic Escherichia coli Reveals emergence of drug resistant O15, O22 and O25 serogroups. Med. 2019; 55:733.
Jan TP and Michael W. Extraintestinal pathogenic Escherichia coli, a common human pathogen: challenges for vaccine development and progress in the field. J Infect Dis.2016; 213(1):6-13.
Makrina TD, Gomes M, Adi I, Benjamin AR, Daniel JW, Minh-Duy P, David LP, Mark A S.Uropathogenic Escherichia coli mediated urinary tract infection. CurrDrug Targets. 2012;13(11):1386-1399.
Mediavilla JR, Patrawalla A, Chen L. Colistin- and Carbapenem-Resistant Escherichia coli Harboring Mcr-1 and Blandm-5, causing a complicated urinary tract infection in a patient from the United States. Mbio. 2016; 7(4):01191-01196
Kudinha T. The Pathogenesis of Escherichia Coli Urinary Tract Infection. In: Samie Amidou, editors. Escherichia coli—Recent Advances on Physiology, Pathogenesis and Biotechnological Applications. InTech. 2017. 45-61 p.
Khameneh B, Iranshahy M, Soheili V, Sedigheh B, Bazzaz F. Review on plant antimicrobials: a mechanistic viewpoint. Antimicrob. Resist InfectControl.2019; 8:118.
Rossiter SE, Fletcher MH, Wuest WM. Natural products as platforms to overcome antibiotic resistance. Chem Rev. 2017; 117(19):12415-12474.
Ana CA, Andrew M, Manuel S. Plants as sources of new antimicrobials and resistance-modifying agents. Nat Prod Rep. 2012; 29(9):1007-1021.
Takó M, Kerekes EB, Zambrano C, Kotogán A, Papp T, Krisch J, Vágvölgyi C. Plant Phenolics andPhenolic-Enriched Extracts as Antimicrobial Agents against Food-Contaminating Microorganisms. Antioxidants (Basel, Switzerland). 2020; 9(2):165.
Lynda B, Valérian F, Pierre L, Yohann C, Lucie L, Nadia O, Pascal D, Claire B.Antibacterial properties of polyphenols: characterization and QSAR (Quantitative Structure–Activity Relationship). Models Front. Microbiol. 2019; 10(2019):829.
Ouerghemmi I, Iness BR, Fatma ZR, Soumaya B,Luisa P, Riadh K, Brahim M, Moufida ST. Antioxidant and antimicrobial phenolic compounds from extracts of cultivated and wild-grown Tunisian Rutachalepensis. J Food Drug Anal. 2017; 25(2):350-359.
Rempe CS, Burris KP, Lenaghan SC, Stewart CN Jr. The Potential of Systems Biology to Discover Antibacterial Mechanisms of Plant Phenolics. FrontMicrobiol.2017; 8:422.
Abreu AC, McBain AJ, Simões M. Plants as sources of new antimicrobials and resistance-modifying agents. Nat Prod Rep.2012; 29:1007-1021.
Dada FA, Nzewuji FO, Esan AM, Oyeleye SI, Adegbola, V.B.Phytochemical and antioxidant analysis of aqueous extracts of unripe pawpaw (Carica papaya Linn.) fruit’s peel and seed. IJRRAS.2016; 27(3):68-71.
Fasoyinu BT, Oyetayo OV, Ajayi AT. Antibacterial Activity of Fermenting Unripe Pawpaw Parts (Caricapapaya) against Some Enteric Bacteria. Int J Pathogen Res. 2019; 3(2):1-10.
Martínez-Ballesta M, Gil-Izquierdo Á, García-Viguera C, Domínguez-Perles R. Nanoparticles and Controlled Delivery for Bioactive Compounds: Outlining Challenges for New "Smart-Foods" for Health. Foods (Basel, Switzerland). 2018; 7(5):72.
Gloria AO, Anthony JA, Emmanuel OA, Samuel WO. Characterization, antibacterial and antioxidant properties of silver nanoparticles synthesized from aqueous extracts of Allium sativum, Zingiberofficinale, and Capsicum frutescens. Pharmacogn. Mag 2017;13(50):201-208.
Okafor F, Janen A, Kukhtareva T, Edwards V, Curley M. Green synthesis of silver nanoparticles, their characterization, application and antibacterial activity. Int J Environ Res Public Health. 2013; 10:5221-5225.
Muhammad N, Bilal H, AbbasiM, Younas WA, Taimoor K.A review of the green syntheses and antimicrobial applications of gold nanoparticles. Green Chem Lett Rev. 2017;10(4):216-227.
Jaison J, Ahmed B, Yen, SC, Alain D, Michael KD.Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein J Nanotechnol. 2018; 9:1050-1074.
Khan I, Saeed K, Khan I. Nanoparticles: Properties, applications and toxicities. Arab J Chem. 2019; 12(7):908-931
Chen J, Qin G, Wang J, Yu J, Shen B, Li S, Ren Y, Zuo L, Shen W, Das B. One-step fabrication of sub-10-nm plasmonic nanogaps for reliable SERS sensing of microorganisms. Biosens Bioelectron.2013;44:191-197.
Beyene HD, Werkneh AA, Bezabh HK, Ambaye TG. Synthesis paradigm and applications of silver nanoparticles, a review. Sustain MaterTechnol.2017;13:18-23.
Ratan ZA, Haidere MF, Nurunnabi M, Shahriar SM, Ahammad AS, Shim YY, Reaney MJ, Cho JY. Green Chemistry Synthesis of Silver Nanoparticles and Their Potential Anticancer Effects. Cancers. 2020; 12:855.
Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv.2009; 27(1):76-83.
Ealia SM and Saravanakumar MP. A review on the classification, characterisation, synthesis of nanoparticles and their application. IOP Conf. Ser.: Mater Sci Eng.2017; 263:032019.
Sivakumar SR, Sridhar V, Abdul K. antioxidant, antimicrobial and sewage treatment of synthesised silver nanoparticles from leaf extract of Hygrophilaauriculata (schumsch) heine. IAJPS. 2017; 4(08):2350-2361.
Olugbemi TI. Biosynthesis of Silver Nanoparticles from aqueous extract of unripe plantain peel and its antibacterial assay: A novel biological approach.IJPSH.2019; 9(5):9-16.
Ana-Alexandra S, Alexandrina N, Rodica-mariana I, Ioana-Raluca S. Green synthesis of silver nanoparticles using plant extracts. Chem Sci. 2016;1:188-193.
Valli JS and Vaseeharan B. Biosynthesis of silver nanoparticles by Cissusquadrangularis extracts. Mater Lett.2012; 82:171-173.
Skiba MI and Vorobyova VI. Synthesis of silver nanoparticles using orange peel extract prepared by plasmochemical extraction method and degradation of methylene blue under solar irradiation. Ann Mater Sci Eng. 2019; 115(2019):1-8.
Borase HP, Salunke BK, Salunkhe RB. ―Plant extract: a promising biomatrix for ecofriendly, controlled synthesis of Silver
Nanoparticles. Appl BiochemBiotech.2014; 173(1):1-29.
Janakiraman V, Govindarajan K, Cr M. Biosynthesis of silver nanoparticles from endophytic fungi, and its cytotoxic activity. BioNanoSci.2019; 9(3):573-579.
Abhay T and Rupa S. Antimicrobial activities of silver nanoparticles synthesized from peel of fruits and vegetables. J Bio Innova Res and DevelopSociety.2016; 1:29-34.
Nooshin A, Gholamreza A, Zahra JA. Green synthesis of silver nanoparticles using Avena sativa L. extract. Nanomed Res J. 2017; 2(1):57-63.
Singleton VL and Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid Reagents. Am J EnolViticult.1965;16:144-158.
Chandra S, Khan S, Avula B, Lata H, Yang MH, ElSohly MA, Khan IA. Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: a comparative study.EvidBased ComplAltern Med. 2014; 2014:253875.
Yu HY, Alyza AA, Nor KM, Fu Siong JY, Su-Yin K, Mohd ZA, Poh WC. Green Synthesis of Silver Nanoparticle Using Water Extract of Onion Peel and Application in the Acetylation Reaction. Arab J Sci Eng.2020;45:4797-4807.
Olugbemi-Adesipe TI. Cost-effective green synthesis of silver nanoparticles from red onion peels aqueous and ethanol extracts and their antimicrobial activity, Proceedings of Nat Develop Conf of The School of Pure and ApplSci, The Federal Polytechnic Ilaro, Ogun State2019; 4:445-450.
Sadegh K, Atefeh Z, Ali Z. Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines. Biotechnol Rep. 2019; 24:e00393.
Kokila T, Ramesh PS, Geetha D. Biosynthesis of silver nanoparticles from Cavendish banana peel extract and its antibacterial and free radical scavenging assay: a novel biological approach. Appl Nanosci.2015;5:911-920.
Balavijayalakshmi J and Ramalakshmi V. Carica papaya peel mediated synthesis of silver nanoparticles and its antibacterial activity against human pathogens. J Appl Res Technol. 2017; 15(5):413-422.
Dada AO, Adekola FA, Dada FE, Adelani-Akande AT, Bello MO, Okonkwo CR, Inyinbor AA, Oluyori AP, Olayanju A, Ajanaku KO, Adetunji CO. Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing. Heliyon. 2019; 5:e02517.
Krithiga N, Rajalakshmi A, Jayachitra. Agreen synthesis of silver nanoparticles using leaf extracts of Clitoria ternatea and Solanum nigrum and study of its antibacterial effect against common nosocomial pathogens. J Nanosci.2015; 2015:8.
Banerjee P, Mantosh S, Aniruddha M, Papita D. Leaf extract mediated green synthesis of Silver Nanoparticles from widely available Indian Plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour Bioprocess.2014;1(3):1-10.
Dada AO, Adekola FA, Adeyemi OS, Bello MO, Adetunji CO, Awakan OJ, Grace FAA. Exploring the effect of operational factors and characterization imperative to the synthesis of silver nanoparticles. In:Silver Nanoparticles Fabrication, Characterization and Applications. London: IntechOpen; 2018. 165-184p.
Xi-Feng Z, Zhi-Guo L, Wei S, Sangiliyandi G.Silver Nanoparticles: Synthesis, Characterization, Properties,Applications, and Therapeutic Approaches. Int J Mol Sci.2016; 17(9):1534.
Das G, Patra JK, Debnath T, Ansari A, Shin HS. Investigation of antioxidant, antibacterial, antidiabetic, and cytotoxicity potential of silver nanoparticles synthesized using the outer peel extract of Ananascomosus (L.). PLoS ONE. 2019;14(8):e0220950.
Mousavi BF and Zaker BS. Green synthesis of silver nanoparticles using Artemisia turcomanica leaf extract and the study of anti-cancer effect and apoptosis induction on gastric cancer cell line (AGS). Artif CellsNanomed Biotechnol. 2018; 46(1):499-510.
Balashanmugam P and Kalaichelvan PT. Biosynthesis characterization of Silver Nanoparticles using Cassia Roxburghii DC. Aqueous Extract, and coated on Cotton Cloth for effective antibacterial activity. Int JNanomed. 2015; 10:87-97.
Hina S, Juan D, Priyanka S, Tae HY. Ecofriendly synthesis of silver and gold nanoparticles by Euphrasia officinalis leaf extract and its biomedical applications. Artif Cells Nanomed Biotechnol. 2018;46(6):1163-1170.
Ghodsieh B, Maryam MT, Mohmmad HN. Green synthesis of silver nanoparticles using aqueous extract of saffron (Crocus sativus L.) wastages and its antibacterial activity against six bacteria. Asian Pac J Trop Biomed. 2017;7(3):227-233.
Sepideh S, Sedigheh EB, Alireza S, Forough Y. Invitro evaluation of antioxidant and antibacterial potential of green synthesized silver nanoparticles using Prosopis farcta fruit extract. Iran J Pharm Res.2019; 18(1):430-455.
Abdel-Aziz MS, Shaheen MS, El-Nekeety AA, AbdelWahhab MA. Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using chenopodiummurale leaf extract. J Saudi ChemSoc.2014;18(4):356-363.
Sultana F, Barman J, Mousmi SB. Biological approach to synthesis of silver nanoparticles using aqueous leaf extract of Houttuynia cordata Thunb and comparative antioxidant study of plant extract and synthesized nanoparticles. J Mater Bio Appl.2015;5:10-16.
Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, Kim SH, Park YK, Park YH, Hwang CY, Kim YK, Lee YS, Jeong DH, Cho MH. Antimicrobial effects of silver nanoparticles. Nanomed. 2007; 3(1):95-101.
Muhammad A, Tariq K, Muhammad A, Ayaz A.K, Muhammad A. Evaluation of the antibacterial potential of silver nanoparticles synthesized through the interaction of antibiotic and aqueous callus extract of Fagonia indica. AMB Express.2019; (9):75.
Philip D. Mangifera indica leaf-assisted biosynthesis of well-dispersed silver nanoparticles. SpectrochimActa A Mol Biomol Spectrosc. 2011; 78(1):327-331.