Phytochemical and Pharmacological Evaluation of Ethanol Leaves Extract of Hydnocarpus kurzii (King) Warb doi.org/10.26538/tjnpr/v5i2.19

Abstract

Hydnocarpus kurzii, is a plant found in the Southeast Asian region. Various parts of the plant such as barks, fruits, leaves have many therapeutic uses in traditional medicinal system. After reviewing various journals and on the basis of traditional uses, our work was designed to conduct various phytochemical and pharmacological tests (antioxidant, antidiabetic, analgesic, laxative, diuretic, antihelminthic, blood anticoagulation) on the leaves of the plant. The extract showed DPPH free radical and Hydrogen peroxide scavenging activities (IC50 values were 58 µg/mL and 67 µg/mL, respectively). Reducing power assay was also performed (RC50 = 79µg/mL). Total phenolic, flavonoid, tannin content and total antioxidant capacities of the plant were found to be 242 mg GAE/g, 365 mg QE/g, 289 mg GAE/g and 274 mg AAE/g, respectively. The plant caused a significant reduction in blood glucose level in oral glucose tolerance test (OGTT) and alloxan-induced diabetic test, though it did not show any response in α-glucosidase enzyme inhibitory test. The extract exhibited significant analgesic, laxative, diuretic, anticoagulant and antihelminthic activities in a dose-dependent manner and these were compared with the respective standard drugs. All of these results signified the plants’ utilization in traditional medicinal system. We hope that these preliminary studies will be helpful for conducting further research on this plant in order to isolate promising drug molecule.