Inhibitory Effect of Leaf, Bark and Twig of Dipterocarpus alatus on the Inflammation Mediators, Nitric Oxide, PGE2, IL-1β and TNF-α in Macrophage RAW 264.7 doi.org/10.26538/tjnpr/v5i2.14
Main Article Content
Abstract
Dipterocarpus alatus has been used in traditional medicine to treat leprosy and gonorrhoea and
for relief of pain but there are limited studies investigating the mechanisms of this activity. In
this work, we screened D. alatus leaf, bark and twig extracts for anti-inflammatory activity using
lipopolysaccharide (LPS) stimulated RAW 264.7 cells and determined their effects on
inflammatory mediators and cytokines. D. alatus extracts were tested for cytotoxicity and effects
on NO, PGE2, IL-1β and TNF-α production in LPS-stimulated RAW 264.7 macrophages using
Griess reaction and ELISA test kits. All extracts were non-toxic to RAW 264.7 cells at a
concentration of 100 µg/mL. Extracts from all tested parts of D. alatus reduced levels of NO,
PGE2, and IL-1β but did not affect TNF-α in LPS-stimulated RAW 264.7 cells. Thus, D. alatus
extracts show specific anti-inflammatory activity with good potential as a treatment for
inflammatory diseases.
Keywords: Dipterocarpaceae, ELISA, Cytokines, Nitric oxide, Prostaglandin E2
Article Details
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