Thermal Stability and Kinetic Characterization of Insect-Derived Glycosidases Using the Arrhenius Model
DOI:
https://doi.org/10.26538/tjnpr/v9i10.42Keywords:
Arrhenius model, Thermal inactivation, β-galactosidase, β-glucosidaseAbstract
Enzymes derived from insects are increasingly recognized as promising alternatives to conventional microbial or plant-derived enzymes, owing to their adaptation to diverse ecological niches and their potential for unique biochemical properties. Among these, glycosidases play a central role in biomass conversion and related industrial processes, where stability under varying environmental conditions is essential. In this study, we investigated the thermal stability and inactivation kinetics of two insect-derived glycosidases, β-glucosidase (Rpbglu) and β-galactosidase (Rpbgal), isolated from the digestive tract of Rhynchophorus palmarum larvae. The primary objective was to evaluate their kinetic and thermodynamic behaviors during heat treatment using the Arrhenius model, to explore their potential for industrial and biotechnological applications. Experimental results demonstrated that both enzymes exhibited a progressive loss of activity in a temperature- and time-dependent manner, following first-order kinetics. Inactivation rate constants (k) increased with rising temperatures. The calculated activation energies (Ea) were 62.78 kJ·mol⁻¹ for β-glucosidase and 54.45 kJ·mol⁻¹ for β-galactosidase. Thermodynamic analyses revealed average activation enthalpies (ΔH#) of 60.01 and 51.64 kJ·mol⁻¹, respectively, and positive Gibbs free energies of activation (ΔG#), indicating that inactivation is a non-spontaneous process requiring external energy input. The negative activation entropies (ΔS#) further suggested a transition to a more ordered molecular state during inactivation. These findings highlight the notable thermal resilience of insect-derived glycosidases and provide fundamental insights into their kinetic properties. Overall, this study underscores their suitability as potential candidates for future biotechnological and industrial applications, particularly in sectors where catalytic efficiency and stability under heat stress are critical.
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