Determination of Antiradical Activity, Total Phenolic and Flavonoid Contents of Kamena-mena (Clerodendrum paniculatum. L) Leaves doi.org/10.26538/tjnpr/v5i2.12
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Abstract
Antioxidants are compounds or molecules that can inhibit or delay-free radical reactions (initiation or propagation reactions) in the body. Kamena-mena (Clerodendrum paniculatum. L) is a plant that has the potential as an antioxidant. This plant is raditionally used to treat rheumatism, ulcers, neuralgia, inflammation, wounds, and antipyretics. This research purpose to investigate the antiradical activity of Kamena-mena leaves using the DPPH radical and ABTS assay and their correlation with phenolics and flavonoids. Kamena-mena leaf powder was extracted using the maceration method with methanol as the solvent. The methanol extract was added with warm water and diffraction with n-hexane, chloroform, and ethyl acetate solvents to obtain fractions. Antioxidant activity was determined using the DPPH and ABTS methods. Kamena-mena leaf extract and fraction showed very strong antioxidant activity using both DPPH and ABTS radicals. The ethyl acetate fraction showed the strongest antioxidant activity with IC50 value of 6,338 ± 0.005 µg/mL sample (DPPH) and 6,231 ± 0.031 µg/mL sample (ABTS), respectively. Phenolic and flavonoids compounds contributed to DPPH radical scavenging by 98.2% and 44.34%, respectively. whereas to ABTS radical, the contribution of phenolic and flavonoids was 98.78% and 36.26%, respectively. Kamena-mena leaves have very strong antioxidant activity, contain high amounts of phenolics and flavonoids, and have the potential to be developed as antioxidants and nutraceuticals.
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