Antioxidant, Hepatoprotective and Toxicological Assessment of Dillenia ovata in Mice

Yen D. H. Nguyen; Tran Chi Linh; Nguyen Trong Tuan; Dai Thi Xuan Trang

doi:10.26538/tjnpr/v9i10.32

Authors

Yen D. H. Nguyen NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, 700000, Vietnam
Tran Chi Linh Faculty of Medicine, Nam Can Tho University, Can Tho City 94000, Vietnam
Nguyen Trong Tuan College of Natural Sciences, Can Tho University, Can Tho City 94000, Vietnam
Dai Thi Xuan Trang College of Natural Sciences, Can Tho University, Can Tho City 94000, Vietnam

DOI:

https://doi.org/10.26538/tjnpr/v9i10.32

Keywords:

Dillenia ovata, Antioxidant Activity, Hepatoprotection, CCl₄–Induced Liver Injury, Medicinal Herbs

Abstract

Dillenia ovata Wall. (DOW), collected from An Giang province, Vietnam, has been used traditionally in folk medicine. This study investigated the antioxidant and hepatoprotective potential of the crude ethanolic extract of Dillenia ovata. The antioxidant potential of the extract was evaluated in vitro using DPPH radical scavenging, reducing power (RP), and total antioxidant capacity (TAC) tests. Hepatoprotective effects were assessed in a Carbon tetrachloride (CCl₄)–induced liver injury model, where mice received Dillenia ovata extract at doses of 100, 200, or 400 mg/kg for 28 days. Biochemical analyses revealed that Dillenia ovata, especially at 400 mg/kg, significantly attenuated CCl₄–induced elevations in serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA), while restoring glutathione (GSH) levels. Histopathological examination further confirmed the protective effect by showing improved hepatic architecture. In vivo safety evaluation showed no signs of toxicity in both acute and sub-chronic studies in mice. These findings suggest that Dillenia ovata extract exhibits potent antioxidant and hepatoprotective activities, highlighting its potential as a promising source of bioactive natural products.

Author Biography

Yen D. H. Nguyen, NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, 700000, Vietnam

^{Center for Hi-Tech Development, Nguyen Tat Thanh University, Saigon Hi-Tech Park, Ho Chi Minh City, 700000, Vietnam}

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