Phenolics Isolation and Chemical Profiling of Livistona australis (R.Br.) Mart. Fruit Extracts; Potent Inhibitors of α-Amylase and Pancreatic Lipase doi.org/10.26538/tjnpr/v5i2.10
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Abstract
Great medicinal value and uses were reported for Palm family species. Livistona australis (R.Br.) Mart. is widely distributed as an ornamental plant in Egypt. The aqueous methanol extract of both pulps and seeds were subjected to chromatographic and spectral analysis as well as LC-ESI-MS technique for isolation and characterization of diagnostic constituents. Thirty-eight compounds were validated for the first time from the fruit parts (pulps and/or seeds) in which 14 compounds were purified and isolated from the pulps and 6 from the seeds. The isolated compounds were mainly phenolics (2 anthocyanins, 1 chlorogenic acid, 2 flavones and 9 flavonols isomers). In addition, some phenolic compounds were recognized putatively beside other minor compounds in both extracts. The phenolic rich extracts of fruit parts were in vitro evaluated for their inhibitory activities on alpha amylase and pancreatic lipase enzymes. The aqueous methanol extracts of pulps and seeds markedly inhibited alpha amylase enzyme with IC50 values of 153.42 ± 6.4 and 134.5 ± 6.1 µg/mL, respectively, higher than that of acarbose (230 ± 3.7 µg/mL). Also, both extracts exhibited significant pancreatic lipase hindrance with IC50 23.03 ± 5.8 and 23.5 ± 4.9 µg/mL, respectively, at 300 ug/mL. The effects were greater than that of orlistat (IC50 24.02 ± 2.1) at 100 ug/mL. Digestive enzymes inhibition activity play a major role in prevention and management of diabetes and obesity, these effects of fruit parts could be attributed to the presence of wide range of phenolic metabolites.
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