The Effect of Ethyl Acetate Extract of Sungkai Leaves (Peronema canescens Jack.) On Uric Acid Levels in Hyperuricemia Male Albino Mice (Mus musculus L.)
DOI:
https://doi.org/10.26538/tjnpr/v9i10.21Keywords:
Peronema canescens Jack, Sungkai leaf, extract, ethyl acetate, gout, hyperuricemiaAbstract
Hyperuricemia is a condition in which the level of uric acid in the blood increases. Uric acid is the end product of purine metabolism. Flavonoid compounds in Peronema canescens (Sungkai) leaves are considered to reduce uric acid levels. This study aims to determine the effect of varying the duration of administration and dose of the ethyl acetate extract of Sungkai leaves on the reduction of uric acid levels in hyperuricemic male albino mice induced by chicken liver juice (0.5 mL/20 g BW per oral) and potassium oxonate. The test animals, comprising as many as 54 mice, were divided into six groups: a negative control group that received only standard feed, a positive control induced with hyperuricemia and a standard control, allopurinol 13 mg/kg BW p.o once daily, and treatment groups I, II, and III were given Peronema canescens (Sungkai) leaf extract at doses of 125 mg/kg BW, 250 mg/kg BW, and 500 mg/kg BW with a duration of administration of 5, 10, and 15 days, respectively, following induction of hyperuricemia. Uric acid levels were measured post-administration of the extracts using an enzymatic method with a photometer at a wavelength of 546 nm from blood serum samples taken via the neck veins of mice. Data were analyzed using Analysis of Variance (ANOVA) and followed up with Duncan's test at a 95% confidence interval. The results showed a dose-dependent reduction in uric acid levels (p<0.05) compared to the negative control, with the optimal decrease among the three doses occurring at 500 mg/kg BW. The reduction in uric acid levels was concentration-dependent. This study showed that ethyl acetate extract from Sungkai leaves reduced uric acid levels in hyperuricemic male albino mice.
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