Unveiling the Bioactive Power of Apis Mellifera (L); Isolates and Their Role In Anti Inflammatory Activity

Biju C. Ramankutty; Jyothisree Gangadharan; Ayswarya Karingali; Arunlal V. Balan; Anilasree B. Parambath; Shitha Gopalakrishnan

doi:10.26538/tjnpr/v9i10.18

Authors

Biju C. Ramankutty Professor, Devaki Amma Memorial College of Pharmacy, Chelembra, Malappuram, Kerala,
Jyothisree Gangadharan Professor, Jamia Salafiya Pharmacy College, Pulikkal, Malappuram, Kerala,
Ayswarya Karingali Associate Professor, Devaki Amma Memorial College of Pharmacy, Malappuram, Kerala,
Arunlal V. Balan Professor, National College of Pharmacy, Manassery, Kozhikode, Kerala,
Anilasree B. Parambath Professor, Devaki Amma Memorial College of Pharmacy, Chelembra, Malappuram, Kerala,
Shitha Gopalakrishnan Assistant Professor, Devaki Amma Memorial College of Pharmacy, Chelembra, Malappuram, Kerala, Shitha Gopalakrishna

DOI:

https://doi.org/10.26538/tjnpr/v9i10.18

Keywords:

Apis mellifera (L.), bioactive constituents, procyanidin, anti-inflammatory activity

Abstract

Apis mellifera (L.), a rich source of several phytochemical elements, is used to treat a wide variety of illnesses and infections. This study investigated the anti-inflammatory effects of the bioactive and metabolic products of Apis mellifera (L.). Seventy percent ethanol was used for continuous Soxhlet extraction of Apis mellifera. The different bioactive components included in the extract were isolated via a column chromatographic technique. Various spectroscopic techniques have been used to describe the structure of the isolated molecule. Studies on the effects of single substances on inflammation have been conducted both acutely and chronically. Using a variety of spectroscopic techniques, the molecular weight of the isolated compound was determined to be 866.74, and procyanidin, a trimer of catechine, was identified as the cause. Acute oral toxicity tests revealed that the extract was safe at concentrations up to 2000 mg/kg. Two hundred mg/kg and 400 mg/kg of isolate were selected for assessment of anti-inflammatory activity. Given that the isolate procyanidins had a significant anti-inflammatory effect at 400 mg/kg, these compounds may be responsible for the anti-inflammatory effect on Apis mellifera (L.).

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