Study of the Antioxidant and Antidiabetic Properties of the Alga Halopteris Scoparia: In Vitro and In Vivo Evaluation in Alloxan-Induced Diabetic Mice

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Published: 2025-09-30
DOI: https://doi.org/10.26538/tjnpr/v9i9.60
Keywords:
Halopteris scoparia, Alloxan-induced diabetic mice, Anti-diabetic, Antioxidant, Oxidative stress, Antioxidant enzymes

Main Article Content

Laabar Abdelmounaim
Laboratory of Pharmacology and Toxicology, Biopharmaceutical and Toxicological Analysis Research Team, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat, Morocco
Imad Kabach
Laboratory of Biochemistry and Molecular Genetics, Faculty of Sciences and Technologies of Tangier, BP 416, 90000 Tangier, Morocco
Soufiane Drioua
Laboratory of Analytical Chemistry, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco
Bitwell Chibuye
Department of Chemistry, Mukuba University, Kitwe, Zambia
Azzouz Krid
Laboratory for Research and Development in Engineering Sciences, Environmental Technologies, Biotechnology and Valorisation of Bio-Resources Team, FSTH, Abdelmalek Essaadi University, Tetouan, Morocco
Ayoub El Hamri
Department of Chemistry, Faculty of Sciences, Mohammed V University, Av. Ibn Battuta. P.O. Box 1014, Rabat, Morocco
Hicham Dahak
Laboratory of Biodiversity, Ecology, and Genome (BioEcoGen), Faculty of Sciences, B.P. 1014, Plant and Microbial Biotechnologies, Biodiversity and Environment (BioBio) Research Center, Mohammed V University in Rabat, Morocco
Hicham Bouchahta
Microbiology and Molecular Biology Team, Center of Plant and Microbial Biotechnology, Biodiversity and Environment, Faculty of Sciences, Mohammed V University, Rabat, Avenue Ibn Battouta, BP 1014, Rabat 10000, Morocco
Moulay E. Faouzi
Laboratory of Pharmacology and Toxicology, Biopharmaceutical and Toxicological Analysis Research Team, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat, Morocco

Abstract

Halopteris scoparia is an edible brown algae seaweed with significant biological activities. This study aimed to explore the biological and toxicological activities of the methanol extract of Halopteris scoparia. The study included a comprehensive evaluation of the phytochemical composition of the extract, as well as its antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, and Ferric reducing antioxidant power (FRAP) assays.  Antidiabetic activity was evaluated in vitro using α-amylase and α-glucosidase inhibitory assays, and in vivo in alloxan-induced diabetic mice. Toxicological effect was investigated in vivo by acute toxicity and sub-acute toxicity tests in mice. Phytochemical analysis revealed a rich phytochemical profile of Halopteris scoparia, with phenolic and flavonoid contents of 55.47 ± 0.70 mg GAE /g and 147.67 ± 1.53 mg QE/g, respectively. LC-MS analysis revealed various bioactive compounds. Halopteris scoparia extract showed remarkable antioxidant potential, with IC50 values of 1.213 ± 0.290 mg/mL and 2.671 ± 0.210 mg/mL in the DPPH and ABTS radical scavenging assays, respectively, and FRAP value of 82.215 ± 0.970 mg AAE/g. Halopteris scoparia extract exhibited promising antidiabetic activity by demonstrating significant α-amylase and α-glucosidase inhibitory activities in vitro, and potent hypoglycaemic effect in vivo comparable to that of glibenclamide. In addition, Halopteris scoparia extract significantly decreased malondialdehyde, and increased the levels of antioxidant enzymes in the pancreas, liver and kidneys of diabetic mice. These results highlight the potentials of H. scoparia extract as a relatively safe natural antioxidant and alternative source of antidiabetic agent(s).

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Article Details

Issue

Vol. 9 No. 9 (2025): Tropical Journal of Natural Product Research (TJNPR)

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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biography

Hicham Bouchahta, Microbiology and Molecular Biology Team, Center of Plant and Microbial Biotechnology, Biodiversity and Environment, Faculty of Sciences, Mohammed V University, Rabat, Avenue Ibn Battouta, BP 1014, Rabat 10000, Morocco

Department of Medical Genetics, National Institute of Health, Rabat, BP 769 Agdal, Rabat

How to Cite

Study of the Antioxidant and Antidiabetic Properties of the Alga Halopteris Scoparia: In Vitro and In Vivo Evaluation in Alloxan-Induced Diabetic Mice. (2025). Tropical Journal of Natural Product Research , 9(9), 4575 – 4585. https://doi.org/10.26538/tjnpr/v9i9.60

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