HPLC Fingerprint Associated with Chemometrics of Guiera senegalensis Leaf Extracts doi.org/10.26538/tjnpr/v5i3.27
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Abstract
Guiera senegalensis is a shrub reported to have several medicinal properties due to its vast phytochemicals. However, the concentration of these phytochemicals is affected by several factors such as location and season of harvest. The aim of this research is to establish a chromatographic fingerprint of the phytochemicals in G. senegalensis leaf samples collected from two states in Northwest Nigeria at two different seasons. HPLC method was adopted and validated for the fingerprint analysis of samples collected. Chemometric techniques such as percent relative standard deviation (% RSD) of the relative retention time (RRT) and relative peak area (RPA), similarity and difference analysis were conducted. The adopted HPLC method was found to be suitable for the analysis as the % RSD of both intraday and interday precisions were less than 3%. Fingerprint analysis showed excellent similarity between the samples as indicated by % RSD of RRT (< 1.6 %), degree of difference (0.00-0.014) and similarity (0.995- 1) values. The high % RSD values of RPA (17-120%) indicated variations in concentrations of the phytochemicals present in the G. senegalensis leaf samples. This method can therefore, be used for the routine quality control of G. senegalensis leaf sample.
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References
Bucar F, Resch M, Bauer R, Burits M, Knauder E, Schubert-Zsilavecz M. 5-methylflavasperone and rhamnetin
from Guiera senegalensis and their antioxidative and 5-lipoxygenase inhibitory activity. Pharmazie. 1998; 53:875-
Ficarra R, Ficarra P, Tommasini S, Carulli M, Melardi S, Di Bella MR, Calabro ML, De Pasquale R, Germano MP, Sanogo R. Casuscelli F. Isolation and characterization of Guiera senegalensis J. F. Gmel., active principles. Boll Chim Farm. 1997; 136(5):454-459.
Males Z, Medic-Saric M, Bucar F. Flavonoids of Guiera senegalensis J.F. Gmel.-thin-layer chromatography and
numerical methods. Croat. Chem Acta. 1998; 71:69-79.
Fiot J, Sanon, S, Azas N, Mahiou V, Jansen O, Angenot L, Balansard G, Ollivier E. Anti-diarrhoeal and ulcerprotective effects of the aqueous root extract of Guiera senegalensis in rodents. J Ethnopharmacol. 2006; 106(2):173-178.
Somboro AA, Patel K, Diallo D. An ethnobotanical and phytochemical study of the African medicinal plant Guiera
senegalensis J. F. Gmel. J Med Plants Res. 2011; 5(9):1639-1651.
Kerharo J and Adam JG. La Pharmacopée Sénégalaise Traditionnelle. Plantes Médicinales et Toxiques. Editions
Vigot Frères, Paris; 1974; 353p.
Kerharo J, Bouquet A, Heintz R. Le Wilinwiga des Mossi (Guiera senegalensis Lam.), ses usages thérapeutiques
indigènes et son application au traitement des diarrhées cholériformes. Acta Tropical. 1948; 5(1):345-348.
Benoit F, Valentin A, Pelissier Y, Diafouka F, Marion C, Kone-Bamba D, Kone M, Mallie M, Yapo A, Bastide JM.
In vitro antimalarial activity of plant extracts used in West African traditional medicine. Am J Trop Hyg. 1996;
(1):67-71.
Ancolio C, Azas N, Mahiou V, Di Giorgio C, Keita A, Timon-David P, Balansard G. Antimalarial activity of
extracts and alkaloids isolated from six plants used in traditional medicine in Mali and Sao Tome. Phytother Res.
; 16(7):646-649.
Azas N, Laurencin N, Delmas F, Di Giorgio C, Gasquet M, Laget M, Timon-David P. Synergistic in vitro antimalarial
activity of plant extracts used as traditional herbal remedies in Mali. Parasitol Res. 2002; 88(2):165-171.
Aniagu SO, Binda LG, Nwinyi FC, Orisadipe A, Wambebe C, Gamaniel K. Anti-diarrhoeal and ulcer-protective effects
of the aqueous root extract of Guiera senegalensis in rodents. J Ethnopharmacol. 2005; 97(3):549-554.
Yi-Zeng L, Peishan X, Kelvin C. Quality control of herbal medicines. J Chromatogr B. 2004; 812:53-70.
Liang YZ, Xie P, Chan K. Quality control of herbal medicines. J Chromatogr B. 2004; 812:53-70.
Pan J, Kai G, Yuan C, Zhou B, Jin R, Yuan Y. Separation and determination of madecassic acid in extracts of Centella
asiatica using high performance liquid chromatography with β-cyclodextrin as mobile phase additive. Se Pu
Chinese. 2007; 25(3):316-318.
Junhua Z, Barbara W, Hongcai S, Xuemei L, Edzard E. Quality of herbal medicine: challenges and solution. Compl
Ther Med. 2012; 20(1):100-106.
Songlin L, Quanbin H, Chunfeng Q, Jingzheng S, Chuen LC, Hongxi X. Chemical markers for the quality control of
herbal medicines: an overview. Chin Med. 2008; 3(7):140-156.
Awwalu S, Musa A, Musa MA, Nasir I, Garba M. Development and validation of Rp-Hplc Method for identification and quantification of quercetin in chloroform extract of Guiera senegalensis Leaf. J Pharm Allied Sci. 2018; 15(1):2709-2717.
Xu Feng, Weijun Kong, Jianhe Wei, Zhen Ou-Yang, Meihua Yang. HPLC fingerprint analysis combined with chemometrics for pattern recognition of ginger. Pharm Biol. 2014; 52(3):362-367.
Liu W, Wang D, Liu J, Li D, Yin D. Quality evaluation of Potentilla fruticosa L. by high performance liquid chromatography fingerprinting associated with chemometric methods. PLoS one. 2016; 11(2):e0149197.
Saeb K, Gholamrezaee S. Variation of essential oil composition of Melissa officinalis L: leaves during different stages of plant growth. Asian Pac J Trop Biomed. 2012; 2(2):547-49.
Santos VM, Bizzo H, Deschamps C. Seasonal variation of vegetative growth, essential oil yield and composition of
menthol mint genotypes at Southern Brazil. J Biosci. 2012; 28(5):790-98.
Mittal K, Malik J, Gautam V. Effect of geographical and seasonal variation on the oil yield and geraniol content of
Pelargonium graveolens. Int J Adv Pharm Res. 2013; 3(3):45-50.