Antioxidant Compound of Endophytic Fungi Penecillium oxalicum Isolated from Swietenia mahagoni L. Jacq doi.org/10.26538/tjnpr/v5i3.20
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Abstract
Endophytic fungi are microorganisms that are symbionts in plant tissues. Endophytic fungi isolated from medicinal plants such as Swietenia mahagoni may produce a plethora of bioactive compounds. In this study, endophytic fungi was isolated from the leaves of S. mahagoni and cultivated in potato-dextrose broth (PDB) for six weeks at static condition. The antioxidant compound was isolated from the ethyl acetate extract of endophytic fungi by chromatographic method, and the chemical structure was determined by spectroscopic analysis. The antioxidant activity was tested by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and the endophytic fungi was analyzed to identify its species by molecular method. Based on the spectroscopic analysis (1H-NMR, 13C-NMR, HMQC, and HMBC spectra), the antioxidant compound was identified as mono-o-methylcurvilinic acid with IC50 of 38.01 µg/mL. Phylogenetic analysis showed the endophytic fungi as Penicillium oxalicum.
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