The Stimulatory Effect of Porcine Placenta Extract on in Vitro Biological Activities of Keratinocytes and Fibroblasts

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Published: 2025-09-30
DOI: https://doi.org/10.26538/tjnpr/v9i9.34
Keywords:
Wound healing, Keratinocyte and fibroblast activities, Porcine Placenta Extract

Main Article Content

Chatchai  Nensat 
Integrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand. 
Worawat  Songjang
Integrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand. 
Jeranan  Inpad
Clinical Research Unit, Faculty of Medicine, Naresuan University, Phitsanulok 65000, Thailand 
Nutthamon  Kijchalao
Department of Biochemistry, Faculty of Medical Sciences, Naresuan University, Phitsanulok 65000, Thailand 
Rutaiwan  Tohtong
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Tuangporn  Suthiphongchai
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand 
Suchada  Pimsen
Department of Biochemistry, Faculty of Medical Sciences, Naresuan University, Phitsanulok 65000, Thailand 
Panthip  Rattanasinganchan
Faculty of Medical Technology, Huachiew Chalermprakiet University, Bangkok 10540, Thailand 
Pornphimon  Metheenukul
Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand 
Tavan  Janvilisri
Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand 
Penpak  Moolthiya
Faculty of Medical Technology, Huachiew Chalermprakiet University, Bangkok 10540, Thailand 
Kittipat  Sopitthummakhun
Faculty of Medical Technology, Huachiew Chalermprakiet University, Bangkok 10540, Thailand 
Arunya  Jiraviriyakul
Integrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand. 

Abstract

Porcine placenta extract (PPE), a synergistic bioactive compound, enhances cellular functions such as proliferation, migration, and cellular signaling. Keratinocytes and fibroblasts play essential roles during wound healing, particularly in tissue regeneration. However, the direct impact of PPE on biological activities of keratinocytes and fibroblasts remains underexplored. This study aimed to elucidate the stimulatory impact of PPE on the proliferation, migration, and gene expression of matrix metalloproteinases (MMPs) and extracellular matrix (ECM)-related proteins in human keratinocytes and fibroblasts. Human keratinocyte (HaCaT) and fibroblast cell lines (Hs 895.Sk) were treated with specific concentrations of PPE (2.5, 5, 10, 12.5, 15, and 50 µg/mL). Cell proliferation and migration were assessed using MTT and scratch assays, respectively. Quantitative real-time PCR was performed to evaluate the expression of MMPs and ECM-related genes. Western blot was employed to investigate the activation of ERK1/2, AKT, JNK signaling pathways, and cyclin D1 expression.  PPE significantly promoted the keratinocyte/fibroblast proliferation and migration in a concentration-dependent manner via activation of ERK1/2, AKT, and JNK signaling pathway. PPE also markedly upregulated the expression of MMP-2, MMP-10, and MMP-14 both in keratinocytes and fibroblasts, as well as α-SMA, fibronectin, collagen I, and collagen III in fibroblasts. Porcine placenta extract (PPE) enhances keratinocyte and fibroblast proliferation/migration by activating JNK, ERK1/2, and PI3K/AKT signaling pathways. PPE also upregulates MMP- and ECM-related gene expression including myofibroblast differentiation.

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Article Details

Issue

Vol. 9 No. 9 (2025): Tropical Journal of Natural Product Research (TJNPR)

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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Chatchai  Nensat , Integrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand. 

Department of Cardio-Thoracic Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand 

Worawat  Songjang, Integrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand. 

Department of Medical Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand 

Arunya  Jiraviriyakul, Integrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand. 

Department of Medical Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand 

How to Cite

The Stimulatory Effect of Porcine Placenta Extract on in Vitro Biological Activities of Keratinocytes and Fibroblasts . (2025). Tropical Journal of Natural Product Research , 9(9), 4353 – 4360. https://doi.org/10.26538/tjnpr/v9i9.34

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