Chemical Properties of Black Rice Yeast Extracts as Pharmaceutical Ingredients for the Management of Type 2 Diabetes mellitus doi.org/10.26538/tjnpr/v5i3.13
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Abstract
Black rice yeast has the potential to be used as an antidiabetic preparation in type 2 Diabetes mellitus. The study aims to identify the chemical composition of black rice yeast extract obtained from different polar solvents as pharmaceutical ingredients for type 2 Diabetes mellitus (DM). The Black rice flour was macerated using polar solvents (distilled water, methanol, and ethanol). The extracts were investigated for the following parameters: amylum, protein and chromium (III) contents, antioxidant activity using the DDPH free radical scavenging assay method and phytochemical profiling using LC-MS. Phytochemical profiling of the different extracts, ethanol, methanol, and water extract, showed 109, 104, and 107 compounds, respectively. The highest concentration of Cr3 + (0.39%) was found in the freeze-dried water extract. This extract also exhibited the most significant antioxidant activity compared to others. Freeze drying may have protected the bioactive constituents in the extracts from the photo-oxidation process resulting from drying under the sun. Black rice yeast extract obtained by maceration using polar solvents could be used as pharmaceutical ingredients for type 2 diabetes mellitus.
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References
Guo H, Ling W, Wang Q, Liu C, Hu Y, Xia M, Feng X, Xia X. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. indica) on hyperlipidemia and insulin resistance in fructose-fed rats. Plant Food Hum Nutr. 2007; 62(1):1-6.
Gradinaru G, Biliaderis C, Kallithraka S, Kefalas P, Garcia-Viguera C. Thermal stability of Hibiscus sabdariffa L. anthocyanins in solution and in solid state: effects of copigmentation and glass transition. Food Chem. 2003; 83(3):423-436.
Yawadio R, Tanimori S, Morita N. Identification of phenolic compounds isolated from pigmented rices and their aldose reductase inhibitory activities. Food Chem. 2007; 101(4):1616-1625.
Sompong R, Siebenhandl-Ehn S, Linsberger-Martin G, Berghofer E. Physicochemical and antioxidative properties of red and black rice varieties from Thailand, China and Sri Lanka. Food Chem. 2011; 124(1):132-140.
Ojokoh AO and Yimin W. Effect of fermentation on chemical composition and nutritional quality of extruded and fermented soya products. Int J Food Eng. 2011;7(4):1-16.
Alebiosu OC, Familoni OB, Ogunsemi OO, Raimi T, Balogun WO, Odusan O, Oguntona SA, Olunuga T, Kolawole BA, Ikem RT. Community based diabetes risk assessment in Ogun state, Nigeria (World Diabetes Foundation project 08-321). Indian J Endocrinol Metab. 2013; 17(4):653-658.
Jach M, Serefko A, Sajnaga E, Kozak E, Poleszak E, Malm A. Dietary Supplements Based on The Yeast Biomass. Curr Top Nutraceutical Res. 2015; 13(2):83-88.
Cefalu WT and Hu FB. Role of chromium in human health and in diabetes. Diabetes Care. 2004; 27(11):2741-2751.
Brasford M. A rapid and sensitive method for quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54.
He F. Bradford protein assay. Bio-protocol. 2011:e45
Nurachman Z, Kono A, Radjasa OK, Natalia D. Identification a novel raw-starch-degrading-α-amylase from a tropical marine bacterium. Am J Biochem Biotechnol. 2010; 6(4):300-306.
Köksal E and Gülçin İ. Antioxidant activity of cauliflower (Brassica oleracea L.). Turk J Agric For. 2008; 32(1):65-78.
Suzuki M, Kimura T, Yamagishi K, Shinmoto H, Yamaki K. Comparison of mineral contents in 8 cultivars of pigmented brown rice. J Jpn Soc Food Sci. 2004; 424-427.
Carbone JW, McClung JP, Pasiakos SM. Recent advances in the characterisation of skeletal muscle and whole-body protein responses to dietary protein and exercise during negative energy balance. Adv Nutr. 2019; 10(1):70-79.
Santos-Sánchez NF, Salas-Coronado R, VillanuevaCañongo C, Hernández-Carlos B. Antioxidant compounds and their antioxidant mechanism. Antioxidants: IntechOpen; 2019; 1-28.
Poljsak B and Milisav I. The neglected significance of "antioxidative stress". Oxid Med Cell. 2012; 2012:1-12.
Çoklar H and Akbulut M. Effect of sun, oven and freezedrying on anthocyanins, phenolic compounds and antioxidant activity of black grape (Ekşikara)(Vitis vinifera L.). South African J Enol Vitic. 2017; 38(2):264-272.
Kereh BC, Mayulu N, Kawengian SE. Gambaran kandungan zat-Zat gizi pada beras hitam (Oryza sativa L.) Varietas Enrekang. eBiomedik. 2016; 4(1):1-7.
Bor T, Aljaloud S, Gyawali R, Ibrahim S. Chapter 26-Antimicrobials from herbs, spices, and plants A2-Watson, Ronald RosS. Fruits, Vegetables, and Herbs: Academic PresS; 2016.
Venugopala KN, Rashmi V, Odhav B. Review on natural coumarin lead compounds for their pharmacological activity. Biomed ResInt. 2013; 2013:1-15.
Pizzorno JE and Katzinger JJ. Glutathione: Physiological and clinical relevance. J Restor Med. 2012; 1(1):24-37.
Panche A, Diwan A, Chandra S. Flavonoids: an overview. J Nutr Sci. 2016; 5:1-15.