Cytotoxicity in Swiss Albino Mice by Extract of Aspergillus tubingensis (CRE4) Endophyte Isolated from Catharanthus roseus doi.org/10.26538/tjnpr/v5i3.11
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Abstract
Cancer represents a significant portion of deaths around the world. The increasing rate of death requires finding new sources of anticancer drugs. Some endophytic fungi represent an important source of bioactive compounds with anticancer activity. The current research aimed to study the in vivo cytotoxicity of extract from the endophytic fungus Aspergillus tubingensis strain CRE4 isolated from Catharanthus roseus. A. tubingensis strain CRE4 was identified using molecular techniques by sequencing of the internal transcribed spacer (ITS) region with accession number MG407714. Ethyl acetate extract was prepared from the fungus and was studied for its in vivo cytotoxicity in Swiss albino mice at doses of 50 and 100 mg/kg after 14 and 28 days from injection with Ehrlich ascites carcinoma (EAC). The body weight and the tumour growth of the Swiss albino mice decreased when treated with A. tubingensis extract. However, the mean survival time and the life span of the mice bearing tumour were increased as compared to positive control groups. Moreover,tubingensis extract improved the biochemical and haematological parameters of tumour bearing mice. The histopathological study showed that in the A. tubingensis extract treated groups, extensive apoptotic cells were noticed in the tumour. The highest improvement was detected in tumourous mice treated with 100 mg/kg of fungal extract after 28 days from EAC injection as compared to other treated groups. In conclusion, A. tubingensis strain CRE4 (MG407714) exhibited effective cytotoxicity on mice bearing Ehrlich Ascites Carcinoma in a dose-dependent manner.
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