Effect of Different Types of Omega-3 Fish Oil on the Physiological, Biochemical and Immunological Parameters in Male Rabbits doi.org/10.26538/tjnpr/v5i3.3
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Abstract
Omega-3 oil has been associated with several health benefits. It is challenging to get the required dose from one source. Therefore, the present study was aimed at evaluating the effects of three different types of omega-3 fish oil on the physiological, biochemical, and immunological parameters of domestic male rabbits. Twenty male rabbits were divided into four groups and used for the study. The grouping included animals that were administered: water (control) (Group 1); 400 mg/kg/day commercial fish oil (Group 2); 400 mg/kg/day pure fish oil (Group 3); 400 mg/kg/day complex omega-3, 6, 9 oil (Group 4). The experiment was terminated after three weeks and blood samples were collected for biochemical and immunological assays. The results indicated that omega-3 oil treatment affected lipid profiles, biochemical parameters and serum cytokine levels differently. There was no significant difference in the levels of cholesterol and VLDL-C among all the treatment groups in relation to the control. A high level of serotonin was observed in Group 2 animals (47.18±12.00 ng/mL). Also, high amounts of glutathione were recorded for Groups 2 (26.32±7.09 µmol/L) and 3 (26.04±7.22 µmol/L) animals in relation to the other experimental groups. There was no significant difference in the cytokine levels of IL-10 and TNF-α among all the treatment groups. Furthermore, the amount of IL-6 was low in both Groups 2 and 3 compared with the other groups. The findings from this study revealed that the commercial omega-3 fish oil has a positive effect on health parameters compared to other omega-3 sources.
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References
Midtb LK. Replacing fish oil with vegetable oils in salmon feed increases hepatic lipid accumulation and reduces insulin sensitivity in mice. Ph.D Thesis, Department of Biology, Faculty of Science, University of Copenhagen. 2013. 232 p.
Gammone M, Riccioni G, Parrinello G, D’Orazio N. Omega-3 polyunsaturated fatty acids: benefits and
endpoints in sport. Nutr. 2018; 11(1):46-51.
Stepankova S and Komers K. Cholinesterases and cholinesterase inhibitors. Curr Enzy Inhib. 2008; 4(4):160-171.
Holas O, Musilek K, Pohanka M, Kuca K. The progress in the cholinesterase quantification methods. Expert Opin Drug Discov. 2012; 7(12):1207-1223.
Mück-Seler D and Pivac N. Serotonin. Periodicum Biologorum. 2011; 113(1):29-41.
Pizzorno J. Glutathione. Integr Med. 2014; 13(1):8-12.
Lutchmansingh FK, Hsu JW, Bennett FI, Badaloo AV, Mcfarlane-Anderson N, Gordon-Strachan GM, WrightPascoe RA, Jahoor F. Boyne MS. Glutathione metabolism in type 2 diabetes and its relationship with microvascular complications and glycemia. PLosOne. 2018; 13(6):e0198626.
Khajehnasiri F, Mortazavi SB, Allameh A, Akhondzadeh S, Hashemi H. Total antioxidant capacity and
malondialdehyde in depressive rotational shift workers. J Env Pub Health. 2013; 10:150693.
Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014; 2014:360438.
Luig M, Kluger MA, Goerke B, Meyer M, Nosko A, Yan I, Steinmetz OM. Inflammation-induced IL-6 functions as a natural brake on macrophages and limits GN. J Am Soc Nephrol. 2015; 26(7):1597-1607.
Toumpanakis D and Vassilakopoulos T. Molecular mechanisms of action of interleukin-6 (IL-6). Pneum. 2007; 20(2):154-167.
Schmidt-Arras D and Rose-John S. IL-6 pathway in the liver: From physiopathology to therapy. J Hepatol. 2016; 64(6):1403-1415.
Ma X, Yan W, Zheng H, Du Q, Zhang L, Ban Y, Wei F. Regulation of IL-10 and IL-12 production and function in macrophages and dendritic cells. F1000Res. 2015; 4:F1000.
Guo J and Yu H. Tumor necrosis factor-α (TNF-α) and its receptor gene polymorphisms association with the susceptibility to papillary thyroid cancer in Chinese Han population. Int J Clin Exp Med. 2018; 11(5):4967-4975.
Subedi L, Lee SE, Madiha S, Gaire BP, Jin M, Yumnam S, Kim SY. Phytochemicals against TNFα-mediated neuroinflammatory diseases. Int J Mol Sci. 2020; 21(3):E764.
Aukrust P. Serum levels of tumor necrosis factor-α (TNF-α) and soluble TNF receptors in human immunodeficiency virus type1infectioncorrelations to clinical immunologic and virologic parameters. J Inf Dis. 1994; 169:420-424.
Hirano T, Akira S, Taga T. Biological and clinical aspects of interleukin 6. Immunol Today. 1990; 11:443-449.
Sedlak J and Lindsay RH. Analytical Biochemistry. 1968; 192p.
Guidet B and Shah S. The level of malondialdehyde afte activation with H2O2 and CuSO4 and inhibition by deferoxamine and molsidomine in the serum of patients with acute myocardial infarction. Nat J Chem. 1989; 5:139-148.
Shahin Z, Leheta TM, Abdel Hay RM, Abdel Aal HM, Rashed LA. Detection of plasma and urinary monoamines and their metabolites in nonsegmental vitiligo. Acta Dermatovenerol Croat. 2012; 20(1):14-20.
Li G, Ma H, Yin Y, Wang J. CRP,IL-2 and TNF-a level in patients with uremia receiving hemodialysis. Mol Med Rep. 2018; 17:3350-3355.
Khalil AK and Kasim SA. Comparison between the Effects of Malathion and Deltamethrin to Cholinesterase Activity in Rabbits. J Nat Sci Res. 2017;7(6):93-114.
Beth Dawson and Robert G. Trapp. Basic and clinical biostatistics, 4
th ed.,Lange Medical Books /McGraw-Hill Medical Publishing Division ,New York. 2004. 83-154 p.
Movahedian A, Rahbani-Nobar M, Dolatkhah N, Mortazavi M, Dolatkhah H. Effect of omega 3 fatty acids on serum lipid profile, malondialdehyde and paraoxonase activity in patients with end stage renal disease under regular hemodialysis. Res Pharm Sci. 2012; 7(5):S914.
Kastelein JJ, Maki KC, Susekov A, Ezhov M, Nordestgaard BG, Machielse BN, Davidson MH. Omega-3 free fatty acids for the treatment of severe hypertriglyceridemia: The Epanova for lowering very high triglyceridEs (EVOLVE) trial. J Clin Lipidol. 2014; 8(1):94-106.
Khan SA and Makki A. Dietary changes with omega-3 fatty acids improves the blood lipid profile of Wistar albino rats with hypercholesterolaemia. Int J Med Res Health Sci. 2017; 6(3):34-40.
Maki KC, Dicklin MR, Lawless A, Reeves MS. Omega-3 fatty acids for the treatment of elevated triglycerides. Clin Lipidol. 2009; 4(4):425-437.
Kromhout D, Yasuda S, Geleijnse JM, Shimokawa H. Fish oil and omega-3 fatty acids in cardiovascular disease: Do they really work? Europ Heart J. 2011; 33(4):436-443.
Abd Allah EA, Gomaa A, Sayed M. The effect of omega-3 on cognition in hypothyroid adult male rats. Acta Physiol Hungarica. 2014; 101(3):362-376.
Barros MP, Marin DP, Bolin AP, Macedo RDCS, Campoio TR, Fineto C, Otton R. Combined astaxanthin and fish oil supplementation improves glutathione-based redox balance in rat plasma and neutrophils. Chem-Bio Interac. 2012; 197(1):58-67.
Firat O, Makay O, Gokce G, Sapaz N, Yenisey C, Coker A. Omega-3 fatty acids inhibit oxidative stress in a rat model of liver regeneration. Ann Surg Treatm Res. 2017; 93(1):1-10.
Shidfar F, Keshavarz A, Hosseyni S, Ameri A, Yarahmadi S. Effects of omega-3 fatty acid supplements on serum lipids, apolipoproteins and malondialdehyde in type 2 diabetes patients. Eastern Mediter ealth J. 2008; 14(2):305-313.
Abdel Aziz N and Yacoub SF. Omega-3 polyunsaturated fatty acids attenuate radiation-induced oxidative stress and organ dysfunctions in rats. Egypt J Rad Sci Appl. 2013; 26(1):41-54.
Thomas J, Thomas CJ, Radcliffe J, Itsiopoulos C. Omega-3 fatty acids in early prevention of inflammatory neurodegenerative disease: A focus on Alzheimer’s disease. BioMed Res Int. 2015; 2015; 172801:1-13.
Jicha G and Markesbery WR. Omega-3 fatty acids: Potential role in the management of early Alzheimer’s disease. Clin Interv Aging. 2010; 5:45-61.
Hamazaki K. Role of omega-3 polyunsaturated fatty acids in mental health studies from Japan. J Oleo Sci. 2019; 68(6):511-515.
Su KP. Biological mechanism of antidepressant effect of omega–3 fatty acids: How does fish oil act as a ‘Mind-Body Interface’? Neurosignals. 2009; 17(2):144-152.
Grosso G, Galvano F, Marventano S, Malaguarnera M, Bucolo C, Drago F, Caraci FX. Omega-3 fatty acids and depression: Scientific evidence and biological mechanisms. Oxid Med Cell Longevity. 2014; 313570:1-16.
Thornberg UB. Effects of omega-3 fatty acids on cognition and behaviour in childhood. Ph.D. Thesis. Department of Behavioural Sciences and Learning, Linköping University. Printed by: LiU-Tryck, Linköping, 2011; 75p.
Patrick RP and Ames BN. Vitamin D and the omega‐3 fatty acids control serotonin synthesis and action, part 2: relevance for ADHD, bipolar disorder, schizophrenia, and impulsive behaviour. FASEB J. 2015; 29(6):2207-2222.
Romieu I, Garcia-Esteban R, Sunyer J, Rios C, AlcarazZubeldia M, Velasco SR, Holguin F. The effect of supplementation with omega-3 polyunsaturated fatty acids on markers of oxidative stress in elderly exposed to PM 2.5. Env Health Perspec. 2008; 116(9):1237-1242.
Meital LT, Windsor MT, Perissiou M, Schulze K, Magee R, Kuballa A, Russell FD. Omega-3 fatty acids decrease oxidative stress and inflammation in macrophages from patients with small abdominal aortic aneurysm. Sci Reports. 2019; 9(1):12978.
Borges MC, Miranda FD, Santos MD, Telles RW, Andrade MV, Correia MITD, Lanna CCD. Omega-3 fatty acids, inflammatory status and biochemical markers of patients with systemic lupus erythematosus: a pilot study. Revista Brasileira de Reumatol (English Edition). 2017; 57(6):526-534.
Lu Y, Chen RG, Wei SZ, Hu HG, Sun F, Yu CH. Effect of omega-3 fatty acids on C-reactive protein and interleukin-6 in patients with advanced nonsmall cell lung cancer. Med(Baltimore). 2018; 97(37):e11971.
Stoodley I, Garg M, Scott H, Macdonald-Wicks L, Berthon B, Wood L. Higher omega-3 index is associated with better asthma control and lower medication dose: A crosssectional study. Nutr. 2019; 12(1):E74.
Luna JM, Moon YP, Liu KM, Spitalnik S, Paik MC, Cheung K, Elkind MS. High-sensitivity C-reactive protein and interleukin-6–dominant inflammation and ischemic stroke risk. Stroke. 2014; 45(4):979-987.
Calder PC. Omega-3 fatty acids and inflammatory processes. Nutr. 2010; 2(3):355-374.
Sundrarjun T, Komindr S, Archararit N, Dahlan W, Puchaiwatananon O, Angthararak S, Chuncharunee S. Effects of n-3 fatty acids on serum interleukin-6, tumour necrosis factor-α and soluble tumour necrosis factor receptor p55 in active rheumatoid arthritis. J Int Med Res. 2004; 32(5):443-454.
Hao W, Wong OY, Liu X, Lee P, Chen Y, Wong KKY. Omega-3 fatty acids suppress inflammatory cytokines production by macrophages and hepatocytes. J Pediatr Surg. 2010; 45(12):2412-2418.
Tan A, Sullenbarger B, Prakash R, Mcdaniel JC. Supplementation with eicosapentaenoic acid and
docosahexaenoic acid reduces high levels of circulating proinflammatory cytokines in aging adults: A randomized, controlled study. Prostaglandins, Leukotrienes and Essential Fatty Acids. 2018; 132:23-29.
Abdolahi M, Tafakhori A, Togha M, Okhovat AA, Siassi F, Eshraghian MR, Djalali M. The synergistic effects of ω-3 fatty acids and nano-curcumin supplementation on tumor necrosis factor (TNF)-α gene expression and serum level in migraine patients. Immunogen. 2017; 69(6):371-378.
Satoh-Asahara N, Shimatsu A, Sasaki Y, Nakaoka H, Himeno A, Tochiya M, Ogawa Y. Highly purified
eicosapentaenoic acid increases interleukin-10 levels of peripheral blood monocytes in obese patients with dyslipidemia. Diabetes Care. 2012; 35(12):2631-2639.