In Vitro Antiviral Activity of Rooibos Tea (Aspalathus linearis) Leaves Aqueous Extract against Influenza Virus doi.org/10.26538/tjnpr/v5i4.16
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Abstract
Influenza virus infection is a major public health problem and its control continues to be a challenge. The emergence of highly resistant-influenza virus strains necessitates the need to search for novel medications, especially from natural sources. The aim of this study was to identify the anti-influenza virus activity of Rooibos tea (Aspalathus linearis) aqueous extract and possible mechanisms of action on different virus strains. Anti-influenza activity was performed using standard in vitro assays including cell-based neutralization by inhibition of virus-induced cytopathic effect (CPE), inhibition of viral plaques and hemagglutination assay. The CPE was determined visually and by dye uptake. The time-of-addition assay was performed to determine the effect on the virus life cycle. The results reveal that the extract showed a significant broad spectrum of anti-influenza activity against both influenza A and B with 50% inhibitory concentration (IC50) of 1.3 mg/mL, while the 50% cytotoxic concentration (CC50) was 40 mg/mL. The inhibitory effect of extract was identified against influenza A/PR/8/34 (H1N1), A/WSN/33 and A/HK/8/68 (H3N2) and influenza B virus strains. The virus-induced cytopathic effects were significantly reduced. Plaque assays indicated that the extract markedly reduced virus infectivity in a dose-dependent manner. Results of this study indicated that the extract directly affected virus particles and disrupts the function of virus adsorption to host cells. Rooibos tea extract strongly inhibits influenza virus replication with a dual mode of action and may contribute to the development of a new anti-influenza virus agent.
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References
Targonski PV and Poland, GA. Influenza. Int Encycl Pub Health (2 Ed.), 2017; (4):238-246.
Cianci R, Newton EE, Pagliari D. Efforts to Improve the Seasonal Influenza Vaccine. Vaccines (Basel). 2020; 8(4):645.
Li YT, Linster M, Mendenhall IH, Su YCF, Smith GJD. Avian influenza viruses in humans: lessons from past outbreaks. Br Med Bull. 2019; 132(1):81-95.
Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team, Dawood FS, Jain S, Finelli L, Shaw MW, Lindstrom S, Garten RJ, Gubareva LV, Xu X, Bridges CB, Uyeki TM. Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med. 2009; 360(25):2605-2615.
De Clercq E and Li G. Approved Antiviral Drugs over the Past 50 Years. Clin Microbiol Rev. 2016; 29(3):695-747.
Vicente D, Cilla G, Montes M, Mendiola J, Pérez-Trallero E. Rapid spread of drug-resistant influenza A viruses in the Basque Country, northern Spain, 2000-1 to 2008-9. Euro Surveill. 2009;14(20):19215.
Long JK, Mossad SB, Goldman MP. Antiviral agents for treating influenza. Cleve Clin J Med. 2000; 67(2):92-95.
Mukherjee PK. Antiviral Evaluation of Herbal Drugs. Quality Control and Evaluation of Herbal Drugs. 2019; 599-628.
Mousa HA. Prevention and Treatment of Influenza, InfluenzaLike Illness, and Common Cold by Herbal, Complementary, and Natural Therapies. J Evid-Based Compl Altern Med. 2017; 22(1):166-174.
Nakano M, Nakashima H, Itoh Y. Anti-human immunodeficiency virus activity of oligosaccharides from rooibos tea (Aspalathus linearis) extracts in vitro. Leukemia.1997; 11(3):128-130.
McGaw LJ, Bagla VP, Steenkamp PA, Fouche G, Olivier J, Eloff JN, Myer MS. Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC. BMC Compl Altern Med. 2013; 13:356.
Damiani E, Carloni P, Rocchetti G, Senizza B, Tiano L, Joubert E, de Beer D, Lucini L. Impact of Cold versus Hot Brewing on the Phenolic Profile and Antioxidant Capacity of Rooibos (Aspalathus linearis) Herbal Tea. Antioxidants (Basel). 2019; 8(10):499.
Sasaki M, Nishida N, Shimada M. A Beneficial Role of Rooibos
in Diabetes Mellitus: A Systematic Review and Meta-Analysis. Molecules. 2018; 23(4):839.
Hendricks R and Pool EJ. The in vitro effects of Rooibos and Black tea on immune pathways. J Immunoassay Immunochem.2010; 31(2):169-180.
Ajuwon OR, Katengua-Thamahane E, Van Rooyen J, Oguntibeju OO, Marnewick JL. Protective Effects of Rooibos (Aspalathus linearis) and/or Red Palm Oil (Elaeis guineensis) Supplementation on tert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats. Evid-Based Compl Altern Med.2013; 2013:984273.
McKay DL and Blumberg JB. A review of the bioactivity of South African herbal teas: rooibos (Aspalathus linearis) and honeybush (Cyclopia intermedia). Phytother Res. 2007; 21(1):1-16.
van der Merwe JD, Joubert E, Richards ES, Manley M, Snijman PW, Marnewick JL, Gelderblom WC. A comparative study on the antimutagenic properties of aqueous extracts of Aspalathus linearis (rooibos), different Cyclopia spp. (honeybush) and Camellia sinensis teas. Mutat Res. 2006; 611(1-2):42-53.
Marnewick JL, Batenburg W, Swart P, Joubert E, Swanevelder S, Gelderblom WC. Ex vivo modulation of chemical-induced mutagenesis by subcellular liver fractions of rats treated with rooibos (Aspalathus linearis) tea, honeybush (Cyclopia intermedia) tea, as well as green and black (Camellia sinensis) teas. Mutat Res. 2004; 558(1-2):145-154.
Huang SH, Kao YH, Muller CJF, Joubert E, Chuu CP. Aspalathin-rich green Aspalathus linearis extract suppresses migration and invasion of human castration-resistant prostate cancer cells via inhibition of YAP signaling. Phytomed. 2020;69:153210.
Santos JS, Deolindo CTP, Esmerino LA, Genovese MI, Fujita A, Marques MB, Rosso ND, Daguer H, Valese AC, Granato D. Effects of time and extraction temperature on phenolic composition and functional properties of red rooibos (Aspalathus linearis). Food Res Int. 2016; 89(1):476-487.
Habu T, Flath RA, Mon TR, Morton JF. Volatile components of rooibos tea (Aspalathus linearis). J Agric. Food Chem. 1985; 33:249-254.
Brauer R and Chen P. Influenza virus propagation in embryonated chicken eggs. J Vis Exp. 2015; (97):52421.
Chen M, Aoki-Utsubo C, Kameoka M, Deng L, Terada Y, Kamitani W, Sato K, Koyanagi Y, Hijikata M, Shindo K, Noda T, Kohara M, Hotta H. Broad-spectrum antiviral agents: secreted phospholipase A2 targets viral envelope lipid bilayers derived from the endoplasmic reticulum membrane. Sci Rep. 2017;7(1):15931.
Karakus U, Crameri M, Lanz C, Yángüez E. Propagation and Titration of Influenza Viruses. In: Yamauchi Y. (eds) Influenza Virus. Methods in Molecular Biology. Humana Press, New York, NY. 2018; vol. 1836.
Daelemans D, Pauwels R, De Clercq E, Pannecouque C. A timeof-drug addiction approach to target identification of antiviral compounds. Nat Protoc. 2011; 6(6):925-933.
Rabe C, Steenkamp JA, Joubert E, Burger JFW, Ferreira D.Phenolic metabolites from rooibos tea (Aspalathus linearis). Phytochem. 1994; 35:1559-1565.
Rahmasaria R, Haruyama T, Charyasriwong S, Nishida T, Kobayashi N. Antiviral Activity of Aspalathus linearis against Human Influenza Virus. Nat Prod Commun. 2017; 12(4):599-602.
Hacioglu M, Dosler S, Birteksoz Tan AS, Otuk G. Antimicrobial activities of widely consumed herbal teas, alone or in combination with antibiotics: an in vitro study. Peer J. 2017;5:e3467.