Antimicrobial Activity and Phytochemical Analysis of Some Selected Plants against Clinical Pathogens doi.org/10.26538/tjnpr/v5i4.22
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Abstract
Plants have been used since ancient times, both as food and medicine, with different plant parts having different antimicrobial potency. This study aimed at assessing the antimicrobial potentials of different metabolites contained in the leaves of Momordica charantia, Nicotiana tabacum, Ocimum gratissimum, and Calotropis procera against clnical isolates. The isolates tested were Escherichia coli, seudomonas aureginosa, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae, and Candida albicans. Selected extracts were analyzed fortheir phytochemical contents and further with as chromatography-mass spectrometry (GC-MS). The methanol extracts of M. charantia, N. tabacum, and O. gratissimum were effective against the clinical isolates. In contrast, the isolates were resistant to the methanol extract of C. procera and the water extracts of all plants under study. Methanol extracts of M. charantia, N. tabacum, and O. gratissimum contained anthocyanins. The GC-MS analysis revealed the presence of 1-methyl-2-phenylbenzylmidazole, 4-phenyl-pyridopyrimidine in M. charantia, tetradecamethyl cycloheptasiloxane in N. tabacum, and 3,4-dimethoxycinnamic acid in O. gratissimum. It was concluded that leaves of M. charantia, N. tabacum, and O. gratissimum could be explored for pharmaceutical applications.
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References
Kupussamy P, Yousuff M, Parine N, Govindan N. Evaluation of in-vitro antioxidant and antibacterial properties of Commelina nudiflora L. extracts prepared by different polar solvents. Saudi J
Biol Sci. 2015; 22(3):293-301.
Al-Lahham S, Sbieh R, Jaradat N, Almasri M, Mosa A, Hamayel
A, Hammad F. Antioxidant, antimicrobial and cytotoxic properties of four different extracts derived from the roots of Nicotiana tabacum L. Eur J Integr Med. 2020; 1(33):101039.
Ali M, Isah T, Mujib A. Climber plants: medicinal importance and conservation strategies. InBiotechnological strategies for the conservation of medicinal and ornamental climbers, Springer, Cham. 2016. 101-138p.
Chouhan S, Sharma K, Guleria S. Antimicrobial activity of some
essential oils-present status and future perspectives. Med. 2017;
(3):58.
Kuspradini H, Putri AS, Mitsunaga T. Chemical composition, antibacterial and antioxidant activities of essential oils of Dryobalanops lanceolata Burck. Leaf. Res J Med Plants. 2018;
(1):19-25.
Doughari JH, Ndakidemi PA, Human IS, Benade S. Antioxidant, antimicrobial and antiverotoxic potentials of extracts of Curtisia dentata. J Ethnopharmacol. 2012; 141(3):1041-1050.
Mahboubi M, Haghi G, Kazempour N, Hatemi AR. Total phenolic content, antioxidant and antimicrobial activities of Blepharis edulis extracts. Songklanakarin J Sci Technol. 2013; 35(1):11-16.
Su BL, Zeng R, Chen JY, Chen CY, Guo JH, Huang CG. Antioxidant and antimicrobial properties of various solvent extracts from Impatiens balsamina L. stems. J Food Sci. 2012; 77(6):614-619.
Sulaiman FA, Nafiu MO, Yusuf BO, Muritala HF, Adeyemi SB, Omar SA, Dosumu KA, Adeoti ZJ, Adegbesan OA, Busari BO, Otohinoyi DA. The GC-MS fingerprints of Nicotiana tabacum L. extract and propensity for renal impairment and modulation of serum triglycerides in Wistar rats. J Pharm Pharmacogn Res. 2020; 8(3):191-200.
Lombardi A, Rossi L, Onelli E, Moscatelli A, Baldi A. Evaluation of Nicotiana tabacum plants transformed for the expression of verocytotoxic Escherichia coli antigens. In Veterinary and Animal Science PhD Course Annual Meeting 2015; (1):1.
Popova V, Petkova Z, Ivanova T, Stoyanova M, Lazarov L, Stoyanova A, Hristeva T, Docheva M, Nikolova V, Nikolov N, Zheljazkov VD. Biologically active components in seeds of three
Nicotiana species. Ind Crops Prod. 2018; 117:375-381.
Weber SS, Kaminski KP, Perret JL, Leroy P, Mazurov A, Peitsch MC, Ivanov NV, Hoeng J. Antiparasitic properties of leaf extracts derived from selected Nicotiana species and Nicotiana tabacum varieties. Food Chem Toxicol. 2019; 132:110660.
Ahamad J, Amin S, Mir SR. Momordica charantia Linn.(Cucurbitaceae): Review on phytochemistry and pharmacology.
Phytochem. 2017; 11(2):53-65.
Zubair MF, Atolani O, Ibrahim SO, Oguntoye OS, Abdulrahim HA, Oyegoke RA, Olatunji GA. Chemical and biological evaluations of potent antiseptic cosmetic products obtained from Momordica charantia seed oil. Sust Chem Pharm. 2018; 9:35-41.
Chimnoi N, Reuk-Ngam N, Chuysinuan P, Khlaychan P, Khunnawutmanotham N, Chokchaichamnankit D, Thamniyom
W, Klayraung S, Mahidol C, Techasakul S. Characterization of essential oil from Ocimum gratissimum leaves: Antibacterial and
mode of action against selected gastroenteritis pathogens. Microb Pathog. 2018; 118:290-300.
Bhatia P, Sharma A, George AJ, Anvitha D, Kumar P, Dwivedi VP, Chandra NS. Antibacterial activity of medicinal plants against ESKAPE: An update. Heliyon. 2021; 7(2):e06310.
Vaghasiya Y, Dave R, Chanda S. Phytochemical analysis of some medicinal plants from western region of India. Res J Med Plant. 2011; 5(5):567-576.
Maher O, Mohammad S, Mohammad A, Enas AZ, Maisa AQ. Antimicrobial Activity of Crude Extracts of Some Plant Leaves. Res J Microbiol. 2012; 7(1):59-62.
Kubmarawa D, Ajoku G, Enwerem N, Okorie D. Preliminary phytochemical and antimicrobial screening of 50 medicinal plants from Nigeria. Afr J Biotechnol. 2007; 6(14):1690-1696.
Mahmood R, Arif M, Mahwish AZA, Qamre A, Marium A, Muhammad A. Evaluation of antimicrobial activity of ethanolic
extracts of Azadirachta indica and Psidium guajava against clinically important at varying pH and temperature. Biomed Res. 2017; (28):134-139.
Harbourne J. Phytochemical methods: A guide to modern techniques of plant analysis. London: Chapman Hall, 1973.
Sofowora A. Medicinal plants and traditional medicine in Africa. Ibadan: Spectrum Books Ltd, 1993.
Mebude O and Adeniyi B. GC-MS Analysis of Phyto Components from the Stem Bark of Cola nitida. J Plant Sci. 2017; 5(4):99-103.
Ameya G, Manilal A, Merdekios B. In-vitro Antibacterial Activity and Phytochemical Analysis of Nicotiana tabacum L. Extracted in Different Organic Solvents. The Open Microbiol J. 2017; 11:352-359.
Sharm Y, Srivastava N, Nagar A, Dua D. Antibacterial Activity, Phytochemical Screening and Antioxidant Activity of Stem of Nicotiana tabacum. Int J Pharm Sci Res. 2017; 7(3):1156-1167.
Nwachukwu I. Antifungal Activities and Phytochemical Constituents of Nicotiana tabacum Leaf Extracts on Selected
Dermatophytes. Nig J Microbiol. 2017; 31(2):3871-3875.
Sarker S and Lim T. Extract of Nicotiana tabacum as a potential control agent of Grapholita molesta (Lepidoptera: Tortricidae).
PLOS One. 2018; 22-60.
Svobodova B, Barros L, Calhelha R, Heleno S, Alves M, Walcott S, Ferreira I. Bioactive Properties and Phenolic Profiles of Momordica charantia L. Medicinal Plants Growing Wild in Trinidad and Tobago. Ind Crops Prod. 2017; 365-373.
Lu Y, Liu Y, Liang W, Cheng K, Liang H, Hou W. Antibacterial and Cytotoxic Activities of Different Wild Bitter Gourd Cultivars. J Bot Stud. 2011; 427-434.
Jagessar R and Mohammed AG. An evaluation of the Antimicrobial and the antifungal activity of leaf extracts of Momordica charantia against Candida albicans, Staphylococcus aureus and Escherichia coli. J Natl Sci. 2008; 6:1-14.
Leelakaprash G, Rose C, Manjunath G. In Vitro Antimicrobial and Antioxidant Activity of Momordica charantia Leaves. Pharmacophore. 2011; 2(4):207-215.
Aguiar JJ, Sousa CP, Araruna MK, Silva MK, Portelo AC, Lopes JC, Carvalho VR, Figueredo FG, Bitu VC, Coutinho HD, Miranda TAS. Antibacterial and modifying-antibiotic activities of the essential oils of Ocimum gratissimum L. and Plectranthus amboinicus L. European J Integr Med. 2015; 7(2):151-156.
Matias EF, Santos KK, Almeida TS, Costa JG, Coutinho HD. Phytochemical screening and modulation of antibiotic activity by
Ocimum gratissimum L. Biomedicine and Preventive Nutr. 2011; 1(1):57-60.
Popova V, Petkova Z, Ivanova T, Stoyanova M, Lazarov L, Stoyanova A, Hristeva T, Docheva M, Nikolova V, Nikolov N Zheljazkov VD. Biologically active components in seeds of three Nicotiana species. Ind Crops Prod. 2018; 117:375-381.
Ali-Esmail AS. Pharmacological and Theraupetic Effects of Jasinum sambac- A Review. Indo Am J Pharm Sci. 2018;
:1766-1778.
Muturi E, Ramirez J, Zilkowski B, Flor-Weiler L, Rooney A. Ovicidal and Larvicidal Effects of Garlic and Asafoetida Essential Oils Against West Nile Virus Vectors. J Insect Sci. 2018; 18(2):128-134.
Omoruyi B, Afolayan A, Bradley G. Chemical Composition Profiling and Antifungal Activity of the Essential Oil and Plant
Extracts of Mesembryanthemum edule (L.) bolus Leaves. Afr J Trad Compl Altern Med. 2014; 11(4):19-30.
Ertutk O, Melek C, Zehra C, Ülkü KKK. Antioxidant, Antimicrobial Activities and Phenolic and Chemical Contents of Physalis peruviana L. from Trazbon, Turkey. Indian J Pharm Edu Res. 2017; 51(3):213-216.
Saisha V. Comparison of phytochemical components in leaves and stems of Exacum bicolor roxb. by gcms. World J Pharm
Pharm Sci. 2017; 6(7):2134-2138.
Fang L, Wang X, Guo L, Liu Q. Antioxidant, Anti-microbial Properties and Chemical Composition of Cumin Essential Oils
Extracted by Three Methods. J Open Chem. 2018; 16:291-297.
Braca A, Siciliano T, D’Arrigo M, Germanò MP. Chemical composition and antimicrobial activity of Momordica charantia
seed essential oil. Fitoterapia. 2008; 79(2):123-125.
Ramalingam R, Palanisamy S, Mohanraj AK, Durisamy S, Rajasekaran N. Chemical Profiling of Momordica charantia L.
Seed Essential Oil and Its Antimicrobial Activity. J Essent Oil Bearing Plants. 2020; 23(2):390-396.
Saliu BK, Usman LA, Sani A, Muhammad NO, Akolade JO. Chemical composition and antibacterial (oral isolates) activity of
leaf essential oil of Ocimum gratissimum L. grown in North central Nigeria. Int J Curr Res. 2011; 33(3):022-028.
Joshi RK. GC-MS analysis of the essential oil of Ocimum gratissimum L. growing desolately in South India. Acta Chromatogr. 2017; 29(1):111-119.