Impact of Acidifier on Florfenicol Pharmacokinetics and their Tissue Residues in Escherichia coli O78-Infected Chickens doi.org/10.26538/tjnpr/v5i4.8
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Abstract
Florfenicol (FF) is a broad-spectrum antibiotic and has been associated with high therapeutic efficacy and low toxicity; therefore, it is widely used in poultry farms. Acidifier has been reported to limit multiplication of enteropathogenic Escherichia coli. The study was conducted to investigate the effect of acidifier on pharmacokinetics of FF and also examine their tissue residues in E. coli O78-infected broiler-chickens. A total of 136 healthy broiler-chickens were used for the study. The broiler-chickens were grouped for pharmacokinetic (A-F), FF tissue residual (G-L) and colony forming unit (CFU; M-N) studies. They were infected with E. coli O78 and FF was administered orally for 3 consecutive days at 30 and 60 mg/kg, with / without acidifier. Faecal CFU of E. coli O78 was determined. At intervals of 1st, 3rd, 5th, 7th and 9th day post FF treatment, chickens were slaughtered and tissue specimens collected for analysis. High performance liquid chromatography (HPLC) was used to measure plasma of FF levels. The results showed that FF serum level was significantly lower in infected broiler-chickens compared with the healthy control group at the different time intervals. The outcome of the CFU showed a significant decrease in infected broiler-chickens with acidifier only (2.77±0.015 CFU/g) in the 4th day after infection in relation to those treated with FF, FF supplemented with acidifier (2.62±0.033 and 2.58±0.036 CFU/g, respectively) while in non-treated infected group was (4.00±0.008 CFU/g). Our findings recommend feed supplementation of acidifier (30 mg/kg BW) with FF for the treatment of coli O78-infected broiler-chickens.
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References
Shen J, Wu X, Hu D, Jiang H. Pharmacokinetics of florfenicol in healthy and Escherichia coli infected broiler chickens. Res Vet Sci. 2002; 73:137-140.
Wei F, Chang K, Shien H, Kuo C, Chen Y, Chou C. Synergism between two amphenicol of antibiotics, florfenicol and thiamphenicol, against Staphylococcus aureus. Vet Record. 2016; 178(13):319.
Ekaterina P, Ricardo R, Aldo M, Betty M, Javiera C. Residue depletion of florfenicol and florfenicol mine in broiler chicken claws and a comparison of their concentrations in edible tissues using LC–MS/MS. Molecules. 2016; 23:2211.
Marta T, Błażej P, Andrzej S, Marcin Ś. Pharmacokinetics of florfenicol and thiamphenicol after single oral and intravenous, as well as multiple oral administrations to geese. J Br Poultry Sci. 2020; 62(1): 25-31.
Zhang J, Gao Y, Zhou Q, Wan Y, Tang M, Tang X, et al. Determination of chloramphenicol, thiamphenicol and florfenicol residues in chicken and egg by high performance liquid chromatography electrospray ionization-tandem mass spectrometry. J Food Safety Qual. 2017; 8(6):2211-2219.
Reuben O, Isaac O, Olufemi A. Food safety impacts of antimicrobial use and their residues in aquaculture. Pub Health Rev. 2018; 39:21.
Samah K, Eslam H, Ola H. Residue withdrawal of florfenicol from the serum and edible tissues of broiler chickens. [J Am Sci. 2012; 8(12): 514-524].
Abudabos M and Al-Mufarrej I. Effects of organic acid supplementation on antioxidant capacity and immune responses of broilers challenged orally with Salmonella enterica subsp. enterica Typhimurium. South Afr J Anim Sci. 2014; 44:342- 349.
Hassanin O, Abdallah F, Awad A. Effects of florfenicol on the immune responses and the interferon-inducible genes in broiler chickens under the impact of E. coli infection. Vet Res Comm. 2014; 38(1):51-58.
Abo-Sreea M. Effect of acidifiers on pharmacokinetic and tissue residues of lincomycin in broiler chickens, MD. 2014; http://www.eulc.edu.eg/eulc_v5/Libraries/Thesis .
Shiroma L, Queiroz N, Jonsson M, Grespan B. Extraction strategies for simultaneous determination of florfenicol and florfenicol amine in Tilapia (Oreochromis niloticus) muscle: Quantification by LC-MS/MS. Food Anal Meth. 2019; 1-12.
USP. United States pharmacopeia 40th ed. USP, physical tests/ (621) Chromatography Rockville. 2017; 508-520.
Clarke R, Peyton D, Healy G, Fenton O, Cummins E. A quantitative microbial risk assessment model for total coliforms and E. coli in surface runoff following application of biosolids to grassland. Environ Poll. 2017; 224:739-750.
Kim Y. Analysis of variance (ANOVA) comparing means of more than two groups, Restorative Dentistry Endodontics. 2014; 39(1):74-77.
Zhang Y, Huo M, Zhou J, Xie S, Solver PK. An add‐in program for pharmacokinetic and pharmacodynamics data analysis in Microsoft Excel. Computer Methods Programs Biomed 2010;99:306-314.
Watteyn A, Croubels S, Baere P, Backer M, Devreese D. Pharmacokinetics of florfenicol in turkey plasma, lung tissue, and pulmonary epithelial lining fluid after single oral bolus or continuous administration in the drinking water. Poult Sci. 2018; 97(4):1134-1140.
Switała M, Hrynyk R, Smutkiewicz A, Jaworski K, Pawlowski P, Okoniewski P, Grabowski T, Debowy J. Pharmacokinetics of florfenicol, thiamphenicol, and chloramphenicol in turkeys. J Vet Pharm Ther. 2007; 30:145-150.
El-Banna A and El-Zorba Y. Pharmacokinetic of florfenicol (water-soluble formulation) in healthy and Pasteurella infected broiler chickens. The J Am Sci. 2011; 7(5):26-32.
Lipi K, Ahmad A, Disha P, Manish K, Richa B. Pharmacokinetics of amoxicillin and oxacillin following single
dose intervenes and intramuscular administratin in sheep. Haryana Vet. 2019; 58(2):185-189.
Sayed E, Aboubakr M, Rabea S. Pharmacokinetics and tissue residues of cephradine in healthy and experimentally Salmonella entretidis infected broiler chickens. W J Pharm Pharm Sci. 2016; 6(6):61-74.
Chang K, Davis L, Cheng N, Shien H, Hsieh K, Koh W, Chou, C. Pharmacokinetics and tissue depletion of florfenicol in Leghorn and Taiwan Native chickens. J Vet Pharm Ther. 2010; 33:471- 479.
Saleh M. Influence of some acidifiers on disposition kinetics and tissue residues of amoxicillin in healthy and experimentally infected broiler chickens with pathogenic E. coli. 2013; http://www.eulc.edu.eg/eulc_v5/Libraries/Thesis
Fascina I, Pasquali I, Carvalho I, Muro I, Vercese I, Aoyagi I, Pezzato I, Gonzales I, Sartori I. Effects of phytogenic additives and organic acids, alone or in combination, on the performance, intestinal quality and immune responses of broiler chickens. Braz J Poult Sci. 2017; 19(3):497-508.
Yousaf S, Goodarzi F, Vahjen W, Männer K, Hafeez A, UrRehman H, Keller S, Peris S, Zentek J. Encapsulated benzoic acid supplementation in broiler diets influences gut bacterial composition and activity. J Br Poult Sci. 2017; 58(2):122-131.